##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632897.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	281055
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.561320737933855	33.0	33.0	33.0	27.0	33.0
2	31.11543292238174	33.0	33.0	33.0	27.0	33.0
3	31.539620359004466	33.0	33.0	33.0	27.0	33.0
4	31.694871110636708	33.0	33.0	33.0	27.0	33.0
5	31.790496522033052	33.0	33.0	33.0	33.0	33.0
6	34.66852395438615	37.0	37.0	37.0	27.0	37.0
7	34.82477806834961	37.0	37.0	37.0	27.0	37.0
8	35.0233157211222	37.0	37.0	37.0	33.0	37.0
9	35.095091708028676	37.0	37.0	37.0	33.0	37.0
10	35.127049865684654	37.0	37.0	37.0	33.0	37.0
11	35.16367259077405	37.0	37.0	37.0	33.0	37.0
12	35.12030741313978	37.0	37.0	37.0	33.0	37.0
13	35.14790699329313	37.0	37.0	37.0	33.0	37.0
14	35.10975431854975	37.0	37.0	37.0	33.0	37.0
15	35.13833591289961	37.0	37.0	37.0	33.0	37.0
16	35.117204817562396	37.0	37.0	37.0	33.0	37.0
17	35.096468662717264	37.0	37.0	37.0	33.0	37.0
18	35.11447581434239	37.0	37.0	37.0	33.0	37.0
19	35.108213694828414	37.0	37.0	37.0	33.0	37.0
20	35.11936097916778	37.0	37.0	37.0	33.0	37.0
21	35.125025350909965	37.0	37.0	37.0	33.0	37.0
22	35.133664229421285	37.0	37.0	37.0	33.0	37.0
23	35.10638487128854	37.0	37.0	37.0	33.0	37.0
24	35.102036967853266	37.0	37.0	37.0	33.0	37.0
25	35.088712173773814	37.0	37.0	37.0	33.0	37.0
26	35.03325327782818	37.0	37.0	37.0	27.0	37.0
27	34.983110067424526	37.0	37.0	37.0	27.0	37.0
28	34.96731956378645	37.0	37.0	37.0	27.0	37.0
29	35.01735247549412	37.0	37.0	37.0	27.0	37.0
30	34.9573357527886	37.0	37.0	37.0	27.0	37.0
31	34.94261265588586	37.0	37.0	37.0	27.0	37.0
32	34.99819964063973	37.0	37.0	37.0	27.0	37.0
33	34.96276529504901	37.0	37.0	37.0	27.0	37.0
34	34.90804290975076	37.0	37.0	37.0	27.0	37.0
35	34.89006066428279	37.0	37.0	37.0	27.0	37.0
36	34.86061447047731	37.0	37.0	37.0	27.0	37.0
37	34.90852324278166	37.0	37.0	37.0	27.0	37.0
38	34.75695148636388	37.0	37.0	37.0	27.0	37.0
39	34.819547775346464	37.0	37.0	37.0	27.0	37.0
40	34.87567557951291	37.0	37.0	37.0	27.0	37.0
41	34.88806817171017	37.0	37.0	37.0	27.0	37.0
42	34.804006333279965	37.0	37.0	37.0	27.0	37.0
43	34.54779313657469	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	7.0
16	9.0
17	12.0
18	18.0
19	19.0
20	43.0
21	73.0
22	215.0
23	467.0
24	923.0
25	1692.0
26	2716.0
27	3899.0
28	5382.0
29	7317.0
30	9384.0
31	11748.0
32	14638.0
33	19134.0
34	26706.0
35	47632.0
36	129019.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.17790112255608	21.183398267243067	13.790539218302467	24.848161391898383
2	15.518670722812262	23.836971411289607	38.34409635124798	22.300261514650156
3	17.69404564942805	27.167280425539488	31.57211222002811	23.566561705004357
4	13.149739374855454	18.23771147995944	38.50918859298002	30.103360552205082
5	13.19136823753358	38.1605023927701	36.00149436942947	12.646635000266851
6	29.361868673391328	39.55417978687446	17.704008112291188	13.379943427443028
7	25.103627403888918	33.14867196812012	24.083186564907226	17.66451406308374
8	23.12963654800662	37.41972211844657	21.033605522050845	18.41703581149597
9	25.006493390973294	16.43201508601519	20.19035420113501	38.3711373218765
10	14.362669228442831	29.32273042642899	34.521357029762854	21.79324331536532
11	32.58259059614666	24.230844496628773	24.89405988151785	18.292505025706713
12	21.618188610770133	26.651367170126843	30.1535286687659	21.576915550337123
13	26.553521552720998	22.660689188948783	27.72019711444379	23.06559214388643
14	21.371973457152514	21.82419811069008	27.466154311433705	29.3376741207237
15	23.352724555691946	29.359378057675546	25.12035010940919	22.16754727722332
16	22.789133799434275	29.608439629254065	24.89405988151785	22.708366689793813
17	22.39597231858533	28.37700805892085	26.15324402696981	23.07377559552401
18	21.105833377808615	28.770169539769796	28.145024995107722	21.97897208731387
19	24.15968404760634	27.40495632527441	27.97103769724787	20.464321929871375
20	23.772571204924304	26.355695504438632	28.67018910889328	21.201544181743788
21	21.792175908629982	28.636387895607623	26.837095942075393	22.734340253687
22	21.466616854352353	28.236466172101547	26.9922257209443	23.304691252601803
23	21.580473572788243	28.557399797192723	27.529842913308784	22.33228371671025
24	23.509633345786412	28.469160840404907	26.306950596858265	21.714255216950416
25	23.18087207130277	27.255875184572414	27.423458042020243	22.13979470210457
26	22.856736226005587	27.55332586148619	27.09434096529149	22.495596947216736
27	22.26610449911939	27.40922595221576	26.98439807155183	23.34027147711302
28	21.236768604009892	27.995232249915496	28.180249417373826	22.587749728700786
29	23.130703954741954	28.096991692017575	26.75277080998381	22.01953354325666
30	21.971500240166517	28.425397164256104	27.303196883172333	22.299905712405046
31	22.860294248456707	27.71201366280621	27.38182917934212	22.04586290939496
32	21.584031595239367	27.861450605753323	27.577876216398927	22.976641582608387
33	22.069345857572362	27.161587589617692	27.931187845795307	22.837878707014642
34	21.754105068402982	27.751151909768552	27.831919019409014	22.662824002419455
35	22.72295458184341	28.12545587162655	26.966963761541336	22.184625784988704
36	22.614079094839088	28.280229848250343	26.572023269466833	22.53366778744374
37	23.32888580526943	26.851328031879877	26.851328031879877	22.968458130970806
38	23.290103360552205	27.51383181227873	27.14628809307787	22.049776734091193
39	22.445073028410807	27.60776360498835	27.57965522762449	22.36750813897636
40	22.51231965273701	27.145576488587643	27.65579690807849	22.686306950596858
41	21.943747665047766	27.529487111063673	28.039351728309402	22.487413495579155
42	22.83716710252442	27.335574887477538	27.221718169041647	22.605539840956396
43	22.355410862642543	26.77340734020032	27.67002899788298	23.201152799274162
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	9.0
2	12.0
3	39.0
4	66.0
5	66.0
6	88.0
7	110.0
8	110.5
9	111.0
10	174.5
11	238.0
12	238.0
13	331.0
14	424.0
15	859.5
16	1295.0
17	1472.5
18	1650.0
19	1650.0
20	1699.5
21	1749.0
22	2179.0
23	2609.0
24	3267.0
25	3925.0
26	3925.0
27	4950.5
28	5976.0
29	8380.5
30	10785.0
31	11518.0
32	12251.0
33	12251.0
34	13654.5
35	15058.0
36	16284.0
37	17510.0
38	19819.0
39	22128.0
40	22128.0
41	23899.5
42	25671.0
43	23552.5
44	21434.0
45	21589.0
46	21744.0
47	21744.0
48	21722.5
49	21701.0
50	22201.5
51	22702.0
52	22844.0
53	22986.0
54	22986.0
55	18789.0
56	14592.0
57	12424.0
58	10256.0
59	8887.5
60	7519.0
61	7519.0
62	6549.5
63	5580.0
64	4624.5
65	3669.0
66	3106.0
67	2543.0
68	2543.0
69	2115.0
70	1687.0
71	1468.0
72	1249.0
73	1029.0
74	809.0
75	809.0
76	616.5
77	424.0
78	330.5
79	237.0
80	194.0
81	151.0
82	151.0
83	117.5
84	84.0
85	72.5
86	61.0
87	48.5
88	36.0
89	36.0
90	25.5
91	15.0
92	8.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	281055.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.34838003333082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.55601393355958	46.357638716309154
2	5.649370691562281	5.914708078221264
3	1.6562449310685579	2.6010521723956397
4	0.8741775873302637	1.8304712223273951
5	0.496353178884814	1.2991642419507037
6	0.3688776134320366	1.1586087296237009
7	0.2564581486311439	0.9397618039031292
8	0.22514649659834163	0.9428843493682411
9	0.18307154057945774	0.8625148721586716
>10	1.3864807605926905	15.241415517486
>50	0.1939030481084049	7.205442769108536
>100	0.14780022548123167	13.549866714561407
>500	0.00610184417124351	2.0964708125861553
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	844	0.30029709487466866	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	735	0.26151465015744246	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	701	0.24941737382362883	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	698	0.24834996708829232	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	696	0.247638362598068	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	614	0.21846257849887032	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	580	0.20636530216505666	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	536	0.1907100033801213	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	503	0.17896852929141985	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	492	0.175054704595186	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	468	0.166515450712494	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	466	0.16580384622226968	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	446	0.15868780132002633	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	438	0.155841383359129	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	436	0.15512977886890467	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	425	0.15121595417267084	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	415	0.14765793172154917	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	401	0.14267670028997884	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	380	0.13520485314262334	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	379	0.13484905089751115	No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG	377	0.13413744640728684	No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT	356	0.12666559925993132	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	348	0.123819181299034	No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG	341	0.12132856558324882	No Hit
ATGTTATTCCTGTTCCTCTTGGTATTCATCAACCACTGCACAA	338	0.12026115884791232	No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG	329	0.11705893864190284	No Hit
TGTTATTCCTGTTCCTCTTGGTATTCATCAACCACTGCACAAA	328	0.11670313639679065	No Hit
CTGTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGC	317	0.11278931170055682	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	315	0.1120777072103325	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	304	0.10816388251409866	No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA	300	0.10674067353365	No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC	300	0.10674067353365	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	284	0.10104783761185535	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.558022451121667E-4	0.0
5	0.0	0.0	0.0	3.558022451121667E-4	0.0
6	0.0	0.0	3.558022451121667E-4	3.558022451121667E-4	0.0
7	0.0	0.0	0.0024906157157851667	3.558022451121667E-4	0.0
8	0.0	0.0	0.0032022202060095	3.558022451121667E-4	0.0
9	0.0	0.0	0.0035580224511216666	3.558022451121667E-4	0.0
10	0.0	0.0	0.003913824696233833	3.558022451121667E-4	0.0
11	0.0	0.0	0.0074718471473554995	7.116044902243334E-4	0.0
12	0.0	0.0	0.008539253882692	7.116044902243334E-4	0.0
13	0.0	0.0	0.009250858372916334	7.116044902243334E-4	0.0
14	0.0	0.0	0.0096066606180285	7.116044902243334E-4	0.0
15	0.0	0.0	0.010318265108252834	0.0010674067353365	0.0
16	0.0	0.0	0.010318265108252834	0.0010674067353365	0.0
17	0.0	0.0	0.011029869598477167	0.0010674067353365	0.0
18	0.0	0.0	0.011385671843589334	0.0010674067353365	0.0
19	0.0	0.0	0.0117414740887015	0.0010674067353365	0.0
20	0.0	0.0	0.012453078578925834	0.0010674067353365	0.0
21	0.0	0.0	0.012808880824038	0.0014232089804486667	0.0
22	0.0	0.0	0.012808880824038	0.0017790112255608333	0.0
23	0.0	0.0	0.012808880824038	0.002134813470673	0.0
24	0.0	0.0	0.012808880824038	0.0028464179608973334	0.0
25	0.0	0.0	0.012808880824038	0.0032022202060095	0.0
26	0.0	0.0	0.012808880824038	0.0032022202060095	0.0
27	0.0	0.0	0.012808880824038	0.004269626941346	0.0
28	0.0	0.0	0.012808880824038	0.010318265108252834	0.0
29	0.0	0.0	0.012808880824038	0.019924925726281333	0.0
30	0.0	0.0	0.012808880824038	0.035936026756328836	0.0
31	0.0	0.0	0.012808880824038	0.09144117699382683	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATAACG	20	0.0018395709	37.0	34
ATAACGA	20	0.0018395709	37.0	35
TAACGAC	25	0.005489367	29.6	36
AACGACA	25	0.005489367	29.6	37
GTATACT	25	0.005489367	29.6	1
CCCATAG	50	2.7084207E-7	29.6	1
GGTATCA	140	0.0	26.42857	1
CCGTTAA	35	8.852664E-4	26.42857	16
TAACGGC	60	1.3314093E-6	24.666666	36
AACGGCC	60	1.3314093E-6	24.666666	37
GTATCAA	375	0.0	23.186665	1
GTCGCCC	40	0.0019275782	23.125002	37
CGCGGCC	40	0.0019275782	23.125002	11
GGCCGTT	40	0.0019275782	23.125002	14
ATTAATC	40	0.0019275782	23.125002	3
CCATAGT	65	2.6708894E-6	22.769228	2
CGTTAAA	50	2.6950694E-4	22.2	17
TCGCCAT	135	1.8189894E-12	20.555555	13
CCTTATA	45	0.0038187278	20.555555	2
TGATAAC	45	0.0038187278	20.555555	33
>>END_MODULE