FastQCFastQC Report
Fri 10 Feb 2017
ERR1632896.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632896.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354502
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8700.24541469441639258No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6140.17320071536972992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3860.108885140281296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAC250.0054912829.631
TACTAGC250.0054912829.67
GGTATCA2100.026.428571
TAGGACC652.6741636E-622.769234
GTATCAA5400.022.2685181
TTTAGGA705.085514E-621.1428592
TATTCCG450.003820669120.55555527
GTTCTAG1001.2822056E-820.351
GTTTAGG656.884935E-519.9230771
TAGCCCT701.21618126E-418.54
ACTAGCA500.007025521718.58
CTACACT801.6115366E-518.54
TACACTA500.007025521718.55
GCGAACT852.7145223E-517.41176623
GACATGT852.7145223E-517.4117667
TTCTATA1005.8547557E-616.6500022
TAGAACT1005.8547557E-616.6500024
CTAGAAC904.4320186E-516.4444453
CGAACTA904.4320186E-516.44444524
GCAATTA803.3743173E-416.187517
Produced by FastQC (version 0.11.5)