FastQCFastQC Report
Fri 10 Feb 2017
ERR1632895.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632895.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369487
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8100.21922286846357245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5950.16103408238990818No Hit
CCTTATACCAGCGTTATGAGATCAAGATGACCAAGATGTATAA4340.11746015421381538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG200.001840319737.04
GGTTACA303.5947937E-430.8333322
TCGTCTA502.7123315E-729.629
CGTCTAC556.242426E-726.9090930
CTCTATA358.8579615E-426.428572
CGGTTCG652.6746711E-622.7692325
ATAGATC502.697296E-422.26
GGTATCA2500.022.1999991
GCTATCT656.8859394E-519.92307737
AGTTCTC656.8859394E-519.9230775
TCCGGTT759.237892E-619.73333423
TTATACC1355.4569682E-1119.1851863
TTAGGTT500.0070260718.54
GTATTAT701.2163581E-418.51
TAGGTAC609.2206534E-418.53
GGTCATG500.0070260718.51
TCGTGTA500.0070260718.513
GGGCGAC500.0070260718.531
GTGTATA500.0070260718.51
CGCAGTT500.0070260718.525
Produced by FastQC (version 0.11.5)