##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632895.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	369487
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60967503592819	33.0	33.0	33.0	27.0	33.0
2	31.139111795543553	33.0	33.0	33.0	27.0	33.0
3	31.5899666294078	33.0	33.0	33.0	27.0	33.0
4	31.72429882512781	33.0	33.0	33.0	27.0	33.0
5	31.835666748762474	33.0	33.0	33.0	33.0	33.0
6	34.67847853916376	37.0	37.0	37.0	27.0	37.0
7	34.86465829650299	37.0	37.0	37.0	27.0	37.0
8	35.052957208237366	37.0	37.0	37.0	33.0	37.0
9	35.128700062519115	37.0	37.0	37.0	33.0	37.0
10	35.19275373693797	37.0	37.0	37.0	33.0	37.0
11	35.18548961127185	37.0	37.0	37.0	33.0	37.0
12	35.16394622814876	37.0	37.0	37.0	33.0	37.0
13	35.178268788888374	37.0	37.0	37.0	33.0	37.0
14	35.14693074451875	37.0	37.0	37.0	33.0	37.0
15	35.163865034493774	37.0	37.0	37.0	33.0	37.0
16	35.15589723048443	37.0	37.0	37.0	33.0	37.0
17	35.132670432247956	37.0	37.0	37.0	33.0	37.0
18	35.14976169662261	37.0	37.0	37.0	33.0	37.0
19	35.14519861321237	37.0	37.0	37.0	33.0	37.0
20	35.14478452557194	37.0	37.0	37.0	33.0	37.0
21	35.15242214205101	37.0	37.0	37.0	33.0	37.0
22	35.148446359411835	37.0	37.0	37.0	33.0	37.0
23	35.16010306181273	37.0	37.0	37.0	33.0	37.0
24	35.141574669744806	37.0	37.0	37.0	33.0	37.0
25	35.133263145929355	37.0	37.0	37.0	33.0	37.0
26	35.07644923908013	37.0	37.0	37.0	33.0	37.0
27	35.02063672064241	37.0	37.0	37.0	27.0	37.0
28	35.001364053403776	37.0	37.0	37.0	27.0	37.0
29	35.049817720244555	37.0	37.0	37.0	27.0	37.0
30	35.01433068010512	37.0	37.0	37.0	27.0	37.0
31	35.00478501273387	37.0	37.0	37.0	27.0	37.0
32	35.0463290995353	37.0	37.0	37.0	27.0	37.0
33	35.016349695659116	37.0	37.0	37.0	27.0	37.0
34	34.96297839978132	37.0	37.0	37.0	27.0	37.0
35	34.95360594554071	37.0	37.0	37.0	27.0	37.0
36	34.93304771209812	37.0	37.0	37.0	27.0	37.0
37	34.95445847891807	37.0	37.0	37.0	27.0	37.0
38	34.83047576775367	37.0	37.0	37.0	27.0	37.0
39	34.900102033359765	37.0	37.0	37.0	27.0	37.0
40	34.954182420491115	37.0	37.0	37.0	27.0	37.0
41	34.97528735787727	37.0	37.0	37.0	27.0	37.0
42	34.877857678348626	37.0	37.0	37.0	27.0	37.0
43	34.6652087894838	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	12.0
16	27.0
17	26.0
18	22.0
19	13.0
20	39.0
21	74.0
22	272.0
23	600.0
24	1190.0
25	2043.0
26	3381.0
27	4919.0
28	6999.0
29	9339.0
30	12073.0
31	15041.0
32	19023.0
33	24441.0
34	34695.0
35	62130.0
36	173127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.37818109974099	19.746026247202202	13.05133874804797	25.824453905008838
2	16.549161404866748	22.33475061368871	37.046770251727395	24.06931772971715
3	18.6818480758457	25.58872166003134	29.42025023884467	26.309180025278287
4	13.38693918865887	17.230646815720174	38.05384221907672	31.328571776544234
5	14.909861510689145	36.24917791424326	34.61150189316539	14.229458681902205
6	31.290410758700578	38.16372429882513	17.05932820369864	13.486536738775653
7	27.69299055176501	31.506656526481308	22.744507925853956	18.05584499589972
8	25.189519523014344	35.38500677967019	20.230481721955034	19.19499197536043
9	26.00497446459551	15.282269741560597	19.680259386663128	39.032496407180766
10	15.369417597912783	27.7002979807138	33.27315981347111	23.657124607902308
11	34.833160571278555	22.77265505958261	23.719373076725297	18.674811292413533
12	23.366992614083852	24.558103532735927	30.255191657622593	21.819712195557624
13	29.019965519761183	21.140933239870417	25.30427322206194	24.534828018306463
14	22.41026071282616	20.13575579113744	27.066446180785793	30.387537315250608
15	24.330761298773705	28.010999033795507	23.647110723787303	24.011128943643484
16	24.016541853975916	27.658618571154058	24.407895271010887	23.916944303859133
17	23.273619910849366	27.12842400409216	25.980345722582932	23.617610362475542
18	24.134002008189732	26.39280948991439	26.537604841307	22.935583660588872
19	25.03768738818957	25.97845120396658	26.245307683355573	22.738553724488277
20	24.791941259097072	25.787375469231666	26.36060267343641	23.06008059823485
21	23.623023272807973	26.338680386590056	26.50052640552983	23.537769935072138
22	23.376194561648987	25.995772517030368	26.40011691886318	24.227916002457462
23	23.783245418648015	25.978721849483204	26.648028212088654	23.590004519780127
24	23.995431503679427	26.50566867034564	25.702663422529078	23.79623640344586
25	23.987853429214017	26.036910635556865	26.369804621001553	23.605431314227566
26	24.2308931031403	26.330290375574783	26.239082836473273	23.199733684811644
27	23.884466841864533	26.200109882079747	25.933794693724	23.98162858233172
28	23.462801126967932	26.337327159006946	26.59768814599702	23.602183568028103
29	24.4641895384682	26.178458240750015	25.83609166222357	23.521260558558218
30	23.776749926249096	26.292670648764364	26.14787529737176	23.782704127614775
31	24.171621735000148	26.027438042475108	26.13434302154068	23.666597200984064
32	23.539393808171873	25.758687044469763	26.491324457964694	24.210594689393673
33	23.272807974299504	26.182247277982718	26.815287141360862	23.72965760635692
34	23.859296808818716	25.65692433022001	26.41797952296021	24.065799338001067
35	23.9564585492859	26.480498637299828	26.19848600898002	23.364556804434255
36	24.23549407692287	26.287257738431936	26.026084814892	23.451163369753196
37	23.95970629548536	25.763288018252332	26.040699672789568	24.236306013472735
38	24.040899950471868	26.170338875251364	26.12541171949216	23.66334945478461
39	23.342093226554656	26.01769480387673	26.63503722729081	24.005174742277806
40	23.608408414910404	26.459388287003332	26.38604335199885	23.546159946087414
41	23.680670767848394	25.708076332861506	26.723267665709482	23.887985233580615
42	23.610844224559997	26.324336174209108	26.531379994424704	23.533439606806194
43	24.08203806899837	25.450151155521034	26.48320509246604	23.98460568301456
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	10.0
2	13.0
3	48.5
4	84.0
5	84.0
6	94.0
7	104.0
8	105.0
9	106.0
10	149.5
11	193.0
12	193.0
13	298.0
14	403.0
15	779.5
16	1156.0
17	1313.5
18	1471.0
19	1471.0
20	1511.0
21	1551.0
22	1804.5
23	2058.0
24	2896.5
25	3735.0
26	3735.0
27	4947.5
28	6160.0
29	7744.0
30	9328.0
31	11092.5
32	12857.0
33	12857.0
34	15005.0
35	17153.0
36	19307.0
37	21461.0
38	22942.0
39	24423.0
40	24423.0
41	25521.5
42	26620.0
43	27563.0
44	28506.0
45	28838.5
46	29171.0
47	29171.0
48	28366.0
49	27561.0
50	27342.5
51	27124.0
52	26184.5
53	25245.0
54	25245.0
55	24306.5
56	23368.0
57	21895.5
58	20423.0
59	18943.0
60	17463.0
61	17463.0
62	15813.0
63	14163.0
64	11783.0
65	9403.0
66	7891.5
67	6380.0
68	6380.0
69	5631.5
70	4883.0
71	3951.5
72	3020.0
73	2376.0
74	1732.0
75	1732.0
76	1319.5
77	907.0
78	714.5
79	522.0
80	411.5
81	301.0
82	301.0
83	236.5
84	172.0
85	164.5
86	157.0
87	110.5
88	64.0
89	64.0
90	48.0
91	32.0
92	19.5
93	7.0
94	3.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	369487.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.31078880758501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.37551770944168	63.021278791389854
2	7.001350465431791	9.98543648816587
3	1.8485194451490234	3.954581392792088
4	0.8240016548172555	2.3504083193509553
5	0.4570742700026927	1.6297163368771554
6	0.2839191374399707	1.2147898589048072
7	0.1878139301973095	0.9375211657995998
8	0.15266098549938661	0.8709100236883646
9	0.10236244241570394	0.6569591861641362
>10	0.6801021774409486	9.302170391979931
>50	0.06147631246118255	2.9968615668207135
>100	0.02443778880297116	2.696794199201583
>500	7.636809000928488E-4	0.38257227886492573
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	810	0.21922286846357245	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	595	0.16103408238990818	No Hit
CCTTATACCAGCGTTATGAGATCAAGATGACCAAGATGTATAA	434	0.11746015421381538	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.706455166216944E-4	0.0	0.0
2	0.0	0.0	2.706455166216944E-4	0.0	0.0
3	0.0	0.0	2.706455166216944E-4	0.0	0.0
4	0.0	0.0	5.412910332433888E-4	0.0	0.0
5	0.0	0.0	5.412910332433888E-4	0.0	0.0
6	0.0	0.0	0.0010825820664867776	0.0	0.0
7	0.0	0.0	0.0024358096495952497	0.0	0.0
8	0.0	0.0	0.0029771006828386384	0.0	0.0
9	0.0	0.0	0.003518391716082027	0.0	0.0
10	0.0	0.0	0.0037890372327037216	0.0	0.0
11	0.0	0.0	0.004871619299190499	0.0	0.0
12	0.0	0.0	0.0051422648158121935	0.0	0.0
13	0.0	0.0	0.005954201365677277	0.0	0.0
14	0.0	0.0	0.005954201365677277	0.0	0.0
15	0.0	0.0	0.006495492398920666	0.0	0.0
16	0.0	0.0	0.006495492398920666	0.0	0.0
17	0.0	0.0	0.00676613791554236	0.0	0.0
18	0.0	0.0	0.00676613791554236	0.0	0.0
19	0.0	0.0	0.007307428948785749	0.0	0.0
20	0.0	0.0	0.007307428948785749	0.0	0.0
21	0.0	0.0	0.007578074465407443	0.0	0.0
22	0.0	0.0	0.007578074465407443	2.706455166216944E-4	0.0
23	2.706455166216944E-4	0.0	0.007578074465407443	8.119365498650832E-4	0.0
24	2.706455166216944E-4	0.0	0.007578074465407443	8.119365498650832E-4	0.0
25	2.706455166216944E-4	0.0	0.007848719982029137	0.0010825820664867776	0.0
26	2.706455166216944E-4	0.0	0.008119365498650832	0.0010825820664867776	0.0
27	2.706455166216944E-4	0.0	0.008119365498650832	0.0016238730997301665	0.0
28	2.706455166216944E-4	0.0	0.008119365498650832	0.007848719982029137	0.0
29	2.706455166216944E-4	0.0	0.008119365498650832	0.01732131306378844	0.0
30	2.706455166216944E-4	0.0	0.008119365498650832	0.0338306895777118	0.0
31	2.706455166216944E-4	0.0	0.008119365498650832	0.09256076668461949	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	20	0.0018403197	37.0	4
GGTTACA	30	3.5947937E-4	30.833332	2
TCGTCTA	50	2.7123315E-7	29.6	29
CGTCTAC	55	6.242426E-7	26.90909	30
CTCTATA	35	8.8579615E-4	26.42857	2
CGGTTCG	65	2.6746711E-6	22.76923	25
ATAGATC	50	2.697296E-4	22.2	6
GGTATCA	250	0.0	22.199999	1
GCTATCT	65	6.8859394E-5	19.923077	37
AGTTCTC	65	6.8859394E-5	19.923077	5
TCCGGTT	75	9.237892E-6	19.733334	23
TTATACC	135	5.4569682E-11	19.185186	3
TTAGGTT	50	0.00702607	18.5	4
GTATTAT	70	1.2163581E-4	18.5	1
TAGGTAC	60	9.2206534E-4	18.5	3
GGTCATG	50	0.00702607	18.5	1
TCGTGTA	50	0.00702607	18.5	13
GGGCGAC	50	0.00702607	18.5	31
GTGTATA	50	0.00702607	18.5	1
CGCAGTT	50	0.00702607	18.5	25
>>END_MODULE