FastQCFastQC Report
Fri 10 Feb 2017
ERR1632894.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632894.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391504
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9680.24725162450447508No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC7190.18365074175487353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7160.18288446605909517No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5060.12924516735461195No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4860.12413666271608975No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4240.10830029833667087No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG3990.10191466753851812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT358.858908E-426.4285726
GGTATCA2650.025.8301891
CGATACA551.8985069E-523.54545436
CTAGACC603.7182133E-521.5833323
ATTAGAC450.00382137120.5555553
AAGGGAC450.00382137120.5555557
GTATCAA6500.020.2076931
GCGATAC656.887285E-519.92307735
TAGACCA701.2165932E-418.54
CGCGATA609.221994E-418.534
TTAACGG1103.832247E-818.535
CTTTATA701.2165932E-418.52
CACACGA500.00702680318.49999814
CGAACTA1351.1441443E-917.81481629
TAGACAG953.5941139E-617.5263165
ATATAGC953.5941139E-617.5263161
TTAGACA752.0628828E-417.2666664
GCGAACT1401.8553692E-917.17857228
CGCGCGA650.001577419917.07692332
ACCCGCA650.001577419917.07692326
Produced by FastQC (version 0.11.5)