##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632894.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	391504
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266061138583513	33.0	33.0	33.0	27.0	33.0
2	30.88799859005272	33.0	33.0	33.0	27.0	33.0
3	31.323720830438514	33.0	33.0	33.0	27.0	33.0
4	31.490403674036536	33.0	33.0	33.0	27.0	33.0
5	31.598890432792512	33.0	33.0	33.0	27.0	33.0
6	34.338880317953326	37.0	33.0	37.0	27.0	37.0
7	34.552566512730394	37.0	37.0	37.0	27.0	37.0
8	34.72769371449589	37.0	37.0	37.0	27.0	37.0
9	34.808017287179695	37.0	37.0	37.0	27.0	37.0
10	34.847324676120806	37.0	37.0	37.0	27.0	37.0
11	34.88674700641628	37.0	37.0	37.0	27.0	37.0
12	34.826137153132535	37.0	37.0	37.0	27.0	37.0
13	34.87287230781806	37.0	37.0	37.0	27.0	37.0
14	34.85166179655891	37.0	37.0	37.0	27.0	37.0
15	34.858703870203115	37.0	37.0	37.0	27.0	37.0
16	34.86150333074502	37.0	37.0	37.0	27.0	37.0
17	34.809741407495196	37.0	37.0	37.0	27.0	37.0
18	34.828042625362706	37.0	37.0	37.0	27.0	37.0
19	34.839626159630555	37.0	37.0	37.0	27.0	37.0
20	34.84955709264784	37.0	37.0	37.0	27.0	37.0
21	34.831319731088314	37.0	37.0	37.0	27.0	37.0
22	34.81869661612653	37.0	37.0	37.0	27.0	37.0
23	34.81430074788508	37.0	37.0	37.0	27.0	37.0
24	34.795751767542605	37.0	37.0	37.0	27.0	37.0
25	34.79583861212146	37.0	37.0	37.0	27.0	37.0
26	34.730620887653764	37.0	37.0	37.0	27.0	37.0
27	34.66842228942744	37.0	37.0	37.0	27.0	37.0
28	34.661283154195104	37.0	37.0	37.0	27.0	37.0
29	34.68542850136908	37.0	37.0	37.0	27.0	37.0
30	34.654353467652946	37.0	37.0	37.0	27.0	37.0
31	34.64073419428665	37.0	37.0	37.0	27.0	37.0
32	34.66811322489681	37.0	37.0	37.0	27.0	37.0
33	34.642792921655975	37.0	37.0	37.0	27.0	37.0
34	34.5868880011443	37.0	37.0	37.0	27.0	37.0
35	34.57837212391189	37.0	37.0	37.0	27.0	37.0
36	34.55677592055254	37.0	37.0	37.0	27.0	37.0
37	34.57806050512894	37.0	37.0	37.0	27.0	37.0
38	34.43283593526503	37.0	37.0	37.0	27.0	37.0
39	34.52694225346357	37.0	37.0	37.0	27.0	37.0
40	34.56270944869018	37.0	37.0	37.0	27.0	37.0
41	34.59016510686992	37.0	37.0	37.0	27.0	37.0
42	34.48757611671911	37.0	37.0	37.0	27.0	37.0
43	34.22950978789489	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	13.0
16	19.0
17	21.0
18	17.0
19	21.0
20	62.0
21	176.0
22	413.0
23	892.0
24	1787.0
25	3106.0
26	4723.0
27	6788.0
28	9291.0
29	12010.0
30	14843.0
31	18211.0
32	22827.0
33	29129.0
34	39685.0
35	68787.0
36	158682.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.93572479463811	20.29532265315297	12.811618782949855	25.957333769259062
2	16.68667497650088	22.803087580203524	36.9825084801177	23.5277289631779
3	18.254730475295272	26.491683354448487	29.650527197678695	25.603058972577546
4	13.261933466835588	17.69994687155176	38.372532592259596	30.665587069353055
5	14.541613878785403	36.673954799950955	34.896450610977155	13.887980710286485
6	30.58155216804937	39.15694347950468	16.941078507499284	13.320425844946667
7	27.374432955985124	31.483969512444315	23.383924557603496	17.757672973967058
8	24.960409089051453	35.50359638726552	20.445001839061668	19.09099268462136
9	26.382105929952182	14.98120070293024	19.795200049041643	38.84149331807593
10	15.399842658057134	27.938922718541832	33.471944092525234	23.1892905308758
11	34.80143242470064	23.082778209162612	23.679706976173932	18.43608238996281
12	23.713933957252035	25.122859536556458	29.53916179655891	21.6240447096326
13	27.79716171482284	21.758142956393804	25.923362213412886	24.52133311537047
14	22.157372593894316	20.34717397523397	27.2528505455883	30.24260288528342
15	24.40204953206098	28.05463034860436	24.152499080469163	23.390821038865504
16	23.465660631819855	27.72921860313049	24.540490007764927	24.264630757284728
17	22.960429523070005	26.94353058972578	25.94558420859046	24.150455678613756
18	23.250337161306142	26.887847889165883	27.168304793820752	22.693510155707223
19	25.15376598961952	25.79871470023295	26.36882381789203	22.678695492255507
20	24.474845723159916	25.97700151211737	26.692703011974334	22.855449752748374
21	23.8848134374106	26.740722955576445	26.44698393886142	22.927479668151538
22	23.357104908251255	26.54455637745719	26.49909068617434	23.59924802811721
23	23.529516939801383	26.42118599043688	26.826801258735543	23.222495811026196
24	23.92619232498263	26.78414524500388	25.549930524336915	23.73973190567657
25	23.717765335730924	26.466140831255874	26.903939678777228	22.91215415423597
26	23.887878540193714	26.679931750378028	26.634721484327105	22.79746822510115
27	24.036791450406636	26.348134374106014	25.897308839756427	23.717765335730924
28	23.08226735869876	26.280957538109444	27.204830601986185	23.431944501205606
29	24.57854836732192	26.210204748865912	26.2707405288324	22.94050635497977
30	24.01048265151825	26.037281866851934	26.866647594916017	23.085587886713803
31	23.54330990232539	26.32514610323266	26.702153745555602	23.429390248886346
32	22.705770566839675	26.145071314724756	26.90930360864768	24.239854509787897
33	23.22428378764968	26.211481875025544	27.06868895336957	23.495545383955207
34	23.75939964853488	26.14660386611631	27.03497282275532	23.059023662593486
35	23.77242633536311	26.42833789693081	26.557327639053497	23.24190812865258
36	24.337937798847523	26.498835260942418	25.911101802280434	23.252125137929625
37	23.414831010666557	25.979044913972782	26.50471004127672	24.10141403408394
38	23.92746945114226	26.0408578200989	26.403561649433975	23.62811107932486
39	23.039866770199026	26.29091912215456	26.85132208100045	23.81789202664596
40	23.27485798357105	26.622971923658508	27.22092239159753	22.881247701172914
41	23.158894928276595	25.412000899096814	27.186184560055583	24.242919612571008
42	22.845743593935183	26.704963423106786	26.829355511054803	23.619937471903224
43	23.77702398953778	25.108811148800527	27.091166373779068	24.022998487882628
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	11.5
2	18.0
3	67.0
4	116.0
5	116.0
6	149.5
7	183.0
8	158.0
9	133.0
10	185.0
11	237.0
12	237.0
13	402.0
14	567.0
15	958.0
16	1349.0
17	1534.0
18	1719.0
19	1719.0
20	1655.5
21	1592.0
22	1853.0
23	2114.0
24	2876.5
25	3639.0
26	3639.0
27	4902.0
28	6165.0
29	7805.0
30	9445.0
31	11735.0
32	14025.0
33	14025.0
34	16763.5
35	19502.0
36	21756.0
37	24010.0
38	26119.5
39	28229.0
40	28229.0
41	29582.0
42	30935.0
43	31656.5
44	32378.0
45	31627.0
46	30876.0
47	30876.0
48	29942.5
49	29009.0
50	29053.5
51	29098.0
52	27855.5
53	26613.0
54	26613.0
55	25929.5
56	25246.0
57	22339.0
58	19432.0
59	17412.5
60	15393.0
61	15393.0
62	14120.0
63	12847.0
64	10369.0
65	7891.0
66	6611.5
67	5332.0
68	5332.0
69	4517.0
70	3702.0
71	3317.0
72	2932.0
73	2879.0
74	2826.0
75	2826.0
76	2597.5
77	2369.0
78	1621.0
79	873.0
80	567.5
81	262.0
82	262.0
83	223.0
84	184.0
85	163.0
86	142.0
87	109.5
88	77.0
89	77.0
90	54.0
91	31.0
92	19.0
93	7.0
94	4.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	391504.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.38724551758729
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.94177851911643	55.488325732429885
2	6.00710443026961	7.495333978820328
3	1.7040908873249112	3.1894060971546727
4	0.8150077811743777	2.0338436217147984
5	0.47266503235323143	1.4744134710499694
6	0.3074707810259888	1.150935306321161
7	0.23296017424581794	1.0173620510545631
8	0.18482158562738674	0.9224407711588447
9	0.13923585308256148	0.7817887216001228
>10	0.9967857550458618	12.464060419797807
>50	0.13013103416204766	5.8044825809808
>100	0.06630093829121424	7.430241257210485
>500	0.0016472282805270618	0.7473659907065905
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	968	0.24725162450447508	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	719	0.18365074175487353	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	716	0.18288446605909517	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	506	0.12924516735461195	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	486	0.12413666271608975	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	424	0.10830029833667087	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	399	0.10191466753851812	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.108504638522211E-4	0.0	0.0
2	0.0	0.0	5.108504638522211E-4	0.0	0.0
3	0.0	0.0	5.108504638522211E-4	0.0	0.0
4	0.0	0.0	5.108504638522211E-4	0.0	0.0
5	0.0	0.0	0.0010217009277044423	0.0	0.0
6	0.0	0.0	0.0020434018554088846	0.0	0.0
7	0.0	0.0	0.003575953246965548	0.0	0.0
8	0.0	0.0	0.00434222894274388	2.554252319261106E-4	0.0
9	0.0	0.0	0.0051085046385222115	5.108504638522211E-4	0.0
10	0.0	0.0	0.005619355102374433	5.108504638522211E-4	0.0
11	2.554252319261106E-4	0.0	0.007918182189709428	5.108504638522211E-4	0.0
12	2.554252319261106E-4	0.0	0.008939883117413871	5.108504638522211E-4	0.0
13	2.554252319261106E-4	0.0	0.010217009277044423	5.108504638522211E-4	0.0
14	2.554252319261106E-4	0.0	0.010472434508970533	5.108504638522211E-4	0.0
15	2.554252319261106E-4	0.0	0.011238710204748866	5.108504638522211E-4	0.0
16	2.554252319261106E-4	0.0	0.012004985900527197	5.108504638522211E-4	5.108504638522211E-4
17	2.554252319261106E-4	0.0	0.012004985900527197	5.108504638522211E-4	5.108504638522211E-4
18	2.554252319261106E-4	0.0	0.012004985900527197	7.662756957783317E-4	5.108504638522211E-4
19	5.108504638522211E-4	0.0	0.01251583636437942	7.662756957783317E-4	5.108504638522211E-4
20	5.108504638522211E-4	0.0	0.01251583636437942	7.662756957783317E-4	5.108504638522211E-4
21	5.108504638522211E-4	0.0	0.01251583636437942	0.0010217009277044423	5.108504638522211E-4
22	5.108504638522211E-4	0.0	0.01251583636437942	0.0012771261596305529	5.108504638522211E-4
23	5.108504638522211E-4	0.0	0.01251583636437942	0.0012771261596305529	5.108504638522211E-4
24	5.108504638522211E-4	0.0	0.01251583636437942	0.0015325513915566634	5.108504638522211E-4
25	5.108504638522211E-4	0.0	0.01251583636437942	0.0015325513915566634	5.108504638522211E-4
26	7.662756957783317E-4	0.0	0.01251583636437942	0.0015325513915566634	5.108504638522211E-4
27	7.662756957783317E-4	0.0	0.01251583636437942	0.0033205280150394377	5.108504638522211E-4
28	7.662756957783317E-4	0.0	0.01251583636437942	0.010472434508970533	5.108504638522211E-4
29	7.662756957783317E-4	0.0	0.01251583636437942	0.022477420409497732	5.108504638522211E-4
30	7.662756957783317E-4	0.0	0.01251583636437942	0.04418856512321713	5.108504638522211E-4
31	7.662756957783317E-4	0.0	0.01251583636437942	0.11136540111978421	5.108504638522211E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	35	8.858908E-4	26.428572	6
GGTATCA	265	0.0	25.830189	1
CGATACA	55	1.8985069E-5	23.545454	36
CTAGACC	60	3.7182133E-5	21.583332	3
ATTAGAC	45	0.003821371	20.555555	3
AAGGGAC	45	0.003821371	20.555555	7
GTATCAA	650	0.0	20.207693	1
GCGATAC	65	6.887285E-5	19.923077	35
TAGACCA	70	1.2165932E-4	18.5	4
CGCGATA	60	9.221994E-4	18.5	34
TTAACGG	110	3.832247E-8	18.5	35
CTTTATA	70	1.2165932E-4	18.5	2
CACACGA	50	0.007026803	18.499998	14
CGAACTA	135	1.1441443E-9	17.814816	29
TAGACAG	95	3.5941139E-6	17.526316	5
ATATAGC	95	3.5941139E-6	17.526316	1
TTAGACA	75	2.0628828E-4	17.266666	4
GCGAACT	140	1.8553692E-9	17.178572	28
CGCGCGA	65	0.0015774199	17.076923	32
ACCCGCA	65	0.0015774199	17.076923	26
>>END_MODULE