FastQCFastQC Report
Fri 10 Feb 2017
ERR1632893.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632893.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390560
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66851.7116448176976649No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51031.3065854158131913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24660.6314010651372388No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13500.3456575174108972No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12900.330294961081524No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG12130.3105796804588284No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11710.2998258910282671No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG11090.2839512494879148No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9760.24989758295780418No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9390.2404240065546907No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7930.20304178615321591No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7620.19510446538303974No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG7420.18998361327324867No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7390.18921548545678No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6930.17743752560426054No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6610.16924416222859484No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6490.16617165096272019No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6410.16412331011880377No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6090.15592994674313806No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5980.15311347808275297No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5930.1518332650553052No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5820.14901679639492013No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5700.14594428512904548No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5610.1436399016796395No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA5550.14210364604670217No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5530.14159156083572308No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5520.14133551823023352No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5480.14031134780827528No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5410.13851904956984842No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5310.1359586235149529No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5260.13467841048750512No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5230.13391028267103647No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5130.1313498566161409No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4930.12622900450634986No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4920.1259729619008603No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4910.12571691929537077No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4900.1254608766898812No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT4880.12494879147890209No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4810.12315649324047523No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4790.12264440802949611No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4740.12136419500204834No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4740.12136419500204834No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4700.12034002458009012No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4660.11931585415813192No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG4660.11931585415813192No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT4550.11649938549774681No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4500.11521917247029906No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4480.11470708725931995No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4480.11470708725931995No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC4470.1144510446538304No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4460.11419500204834085No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT4440.11368291683736173No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT4320.1106104055714871No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG4320.1106104055714871No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4250.10881810733306022No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4210.10779393691110202No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4190.1072818517001229No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4140.10600163867267513No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4110.10523351085620647No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4100.10497746825071691No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC4100.10497746825071691No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG4050.10369725522326916No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4020.10292912740680049No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3950.1011368291683736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTTT251.2321338E-436.99999616
TATCTTC303.5951662E-430.83333437
GCCTAGT358.8588696E-426.4285721
GGTATCA9300.025.4623641
GTATCAA26100.023.8869721
AGTGACA1250.023.6823
CGGGGAC400.001928916923.12500217
TTAACGG1300.022.7692335
CACATGT1350.021.92592628
AACGGCC1350.021.92592637
ATGTTTA1350.021.92592631
CTGGTAT603.718183E-521.58333433
TCAAGGG603.718183E-521.5833343
GACACAT1400.021.14285926
GTTTAAC1400.021.14285933
CATGTTT1400.021.14285930
CAGTATT806.935934E-720.812514
GCTGGTA450.003821353420.55555732
CAGTTGC450.003821353420.55555720
GGTCATT450.003821353420.55555719
Produced by FastQC (version 0.11.5)