FastQCFastQC Report
Fri 10 Feb 2017
ERR1632892.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632892.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450681
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16610.368553367015694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12050.2673731530727943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6000.1331318604511839No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCT400.001929375623.12533
GGTATCA3500.021.6714291
GTATCAA9650.021.6632121
TATACTG450.003822253320.5555575
GTATTAG1950.019.9230771
CGAGTCG656.890234E-519.92307721
TATTAGC951.6704689E-719.4736842
TTAGCTA500.007028408318.54
AGGACCG500.007028408318.55
GCCGTCC701.21711135E-418.527
GCGTAAT500.007028408318.51
TGTGCAA1258.536517E-917.7610
TTAACGG1156.379378E-817.6956535
GAGTCGC752.0637552E-417.26666622
TTTCGGG650.001577919317.07692322
CGCCGTC803.3768604E-416.187526
TCCGCCG700.002589215815.85714231
CGCGCGA700.002589215815.85714236
GTGTACA700.002589215815.8571421
TCGCCGT957.044463E-515.57894825
Produced by FastQC (version 0.11.5)