##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632892.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	450681
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56346506730925	33.0	33.0	33.0	27.0	33.0
2	31.10211657469474	33.0	33.0	33.0	27.0	33.0
3	31.545032961229783	33.0	33.0	33.0	27.0	33.0
4	31.69297574115616	33.0	33.0	33.0	27.0	33.0
5	31.81651988879052	33.0	33.0	33.0	33.0	33.0
6	34.644114129506235	37.0	37.0	37.0	27.0	37.0
7	34.822399879293776	37.0	37.0	37.0	27.0	37.0
8	35.01263199469248	37.0	37.0	37.0	27.0	37.0
9	35.10183256005911	37.0	37.0	37.0	33.0	37.0
10	35.12738278294403	37.0	37.0	37.0	33.0	37.0
11	35.167502069091	37.0	37.0	37.0	33.0	37.0
12	35.104111333737166	37.0	37.0	37.0	33.0	37.0
13	35.14618765823276	37.0	37.0	37.0	33.0	37.0
14	35.11203267943401	37.0	37.0	37.0	33.0	37.0
15	35.119528003177415	37.0	37.0	37.0	33.0	37.0
16	35.137891324462316	37.0	37.0	37.0	33.0	37.0
17	35.08234649341774	37.0	37.0	37.0	33.0	37.0
18	35.09632090103643	37.0	37.0	37.0	33.0	37.0
19	35.11192395508131	37.0	37.0	37.0	33.0	37.0
20	35.10406917531469	37.0	37.0	37.0	33.0	37.0
21	35.097792008094416	37.0	37.0	37.0	33.0	37.0
22	35.092091745602765	37.0	37.0	37.0	33.0	37.0
23	35.08352027265405	37.0	37.0	37.0	33.0	37.0
24	35.08741659843659	37.0	37.0	37.0	33.0	37.0
25	35.09350959991657	37.0	37.0	37.0	33.0	37.0
26	35.01966579465298	37.0	37.0	37.0	27.0	37.0
27	34.96683019696859	37.0	37.0	37.0	27.0	37.0
28	34.95062139295866	37.0	37.0	37.0	27.0	37.0
29	34.994994242047035	37.0	37.0	37.0	27.0	37.0
30	34.948582256629415	37.0	37.0	37.0	27.0	37.0
31	34.94930782526887	37.0	37.0	37.0	27.0	37.0
32	34.97188255107271	37.0	37.0	37.0	27.0	37.0
33	34.921978073182586	37.0	37.0	37.0	27.0	37.0
34	34.86470918454516	37.0	37.0	37.0	27.0	37.0
35	34.876768712237705	37.0	37.0	37.0	27.0	37.0
36	34.83117105003317	37.0	37.0	37.0	27.0	37.0
37	34.86229062241364	37.0	37.0	37.0	27.0	37.0
38	34.731395377218035	37.0	37.0	37.0	27.0	37.0
39	34.79622393666474	37.0	37.0	37.0	27.0	37.0
40	34.82936267559538	37.0	37.0	37.0	27.0	37.0
41	34.84255604296609	37.0	37.0	37.0	27.0	37.0
42	34.738899576418795	37.0	37.0	37.0	27.0	37.0
43	34.51019013448537	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	10.0
16	22.0
17	27.0
18	16.0
19	18.0
20	27.0
21	107.0
22	329.0
23	702.0
24	1451.0
25	2742.0
26	4168.0
27	6508.0
28	8797.0
29	11931.0
30	15137.0
31	19146.0
32	24275.0
33	30814.0
34	43119.0
35	76666.0
36	204668.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.19454780654166	20.343213936243153	13.29410381178705	25.16813444542814
2	16.067462351419294	23.267233364619322	37.378544913142555	23.286759370818828
3	17.93463669424715	26.781470707662404	30.352510977831326	24.931381620259117
4	12.727183972699093	17.882937155105274	39.01584491025803	30.374033961937602
5	14.19718159851425	36.70112562988012	35.18075978352759	13.920932988078041
6	29.817764671685737	39.8800925710203	17.19531109587491	13.106831661419053
7	26.57933216621069	32.24409282840857	23.336240045619853	17.840334959760895
8	24.562384480375254	35.58170856991975	20.726855580776647	19.129051368928355
9	26.30641185228576	14.89346122867394	20.30127740020103	38.498849518839265
10	14.960470931767702	27.972778972266415	33.96326891970152	23.103481176264363
11	34.37952787004555	23.517077489399377	23.934002099045667	18.169392541509406
12	22.990540981314943	25.604141288405767	30.38601582937821	21.01930190090108
13	28.20043445363794	22.338638637972313	25.87972423953972	23.58120266885003
14	22.03997949769349	20.735509151705976	27.815683376934018	29.40882797366652
15	24.06424943585374	28.444731417565862	24.435465440078456	23.05555370650194
16	23.188685566953122	28.304055418355777	25.06051952489677	23.446739489794332
17	22.518588536015496	27.108531311504148	26.521863579782597	23.851016572697763
18	23.24415717547445	26.87044716773061	27.69298017888484	22.192415477910096
19	24.686197110594854	26.250496470896266	27.077910983600372	21.985395434908504
20	24.4982593009246	26.051908112389917	27.17975685684553	22.270075729839952
21	23.387495811893558	26.928359527026878	27.138264093671577	22.545880567407988
22	23.12123209099119	26.689609723951087	27.160008964211933	23.029149220845788
23	22.97744968170391	26.667864853410727	27.72426616609087	22.63041929879449
24	23.450955332041953	26.857133981685493	26.465060652656756	23.226850033615793
25	23.395483723520627	26.735096442938577	27.32198606109421	22.54743377244659
26	23.198670456486962	26.962086265007844	27.131163727780848	22.708079550724346
27	23.297853692523095	26.78790541425088	26.98671565919131	22.927525234034714
28	22.769098320097807	26.765716770842346	27.392324060699252	23.07286084836059
29	23.89472820021257	26.656770531706464	26.738202853015768	22.710298415065203
30	23.665519513802447	26.424677321653235	27.344396590936825	22.565406573607497
31	23.45162099134421	26.52275112551894	27.089005305304635	22.936622577832214
32	22.42428680152924	26.55603409063173	27.540544198668236	23.47913490917079
33	22.648613986389485	26.586432532101416	27.757549131203668	23.00740435030543
34	23.02715224293902	26.247390060819072	27.655037598656257	23.070420097585654
35	22.95992065341117	26.911052385168222	27.188188541340768	22.940838420079835
36	23.657531602175375	26.63280679682525	26.986271886323145	22.723389714676234
37	23.152074305329045	26.30263978290631	27.15712444056883	23.38816147119581
38	23.062210299524498	26.520310374744	27.12783543126957	23.289643894461936
39	22.79550280575396	26.321722016237647	27.6135448354823	23.269230342526086
40	22.823238610014624	26.927028208422364	27.559404545565492	22.690328635997524
41	23.16449994563782	26.078312598046065	27.448461328522832	23.30872612779327
42	22.535673791440065	26.795227666575695	27.57449282308329	23.09460571890095
43	23.076854804174125	26.00864025774328	27.530337422700313	23.384167515382277
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	16.0
2	27.0
3	88.0
4	149.0
5	149.0
6	214.0
7	279.0
8	279.5
9	280.0
10	374.0
11	468.0
12	468.0
13	687.5
14	907.0
15	1586.5
16	2266.0
17	2596.0
18	2926.0
19	2926.0
20	2730.5
21	2535.0
22	2902.0
23	3269.0
24	4424.5
25	5580.0
26	5580.0
27	7215.0
28	8850.0
29	10786.5
30	12723.0
31	15098.0
32	17473.0
33	17473.0
34	20264.0
35	23055.0
36	25292.0
37	27529.0
38	29705.5
39	31882.0
40	31882.0
41	33292.5
42	34703.0
43	35534.5
44	36366.0
45	35711.5
46	35057.0
47	35057.0
48	34368.5
49	33680.0
50	33522.0
51	33364.0
52	31830.0
53	30296.0
54	30296.0
55	28831.0
56	27366.0
57	24343.5
58	21321.0
59	19688.5
60	18056.0
61	18056.0
62	15831.0
63	13606.0
64	11362.5
65	9119.0
66	7897.0
67	6675.0
68	6675.0
69	5512.5
70	4350.0
71	3582.0
72	2814.0
73	2260.5
74	1707.0
75	1707.0
76	1271.0
77	835.0
78	655.5
79	476.0
80	375.0
81	274.0
82	274.0
83	234.5
84	195.0
85	164.0
86	133.0
87	94.0
88	55.0
89	55.0
90	41.5
91	28.0
92	15.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	450681.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.3410980691876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.05788578654047	60.86313706372469
2	6.3967986511117125	8.74328487888945
3	1.6464825998186157	3.375672864702449
4	0.7906552092136849	2.1613698076714587
5	0.4446565499900506	1.5194158444988328
6	0.3054007481998022	1.2522853489875565
7	0.21649456310595364	1.0356833318068928
8	0.15633047497670463	0.8547037057268518
9	0.11735397382589961	0.7218089490640212
>10	0.7448208834376973	9.791404686562831
>50	0.07739939707791445	3.6895237454288488
>100	0.04474142350076019	5.218139554914996
>500	3.265797335821912E-4	0.1339129056008981
>1k	6.531594671643824E-4	0.63965731242029
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1661	0.368553367015694	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1205	0.2673731530727943	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	600	0.1331318604511839	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.656593022559194E-4	0.0	0.0
2	6.656593022559194E-4	0.0	8.875457363412258E-4	0.0	0.0
3	6.656593022559194E-4	0.0	0.0015532050385971452	0.0	0.0
4	6.656593022559194E-4	0.0	0.0017750914726824517	0.0	0.0
5	6.656593022559194E-4	0.0	0.0031064100771942904	0.0	0.0
6	6.656593022559194E-4	0.0	0.004215842247620822	0.0	0.0
7	6.656593022559194E-4	0.0	0.0068784794566445	0.0	0.0
8	6.656593022559194E-4	0.0	0.007766025192985726	0.0	0.0
9	6.656593022559194E-4	0.0	0.009763003099753484	0.0	0.0
10	8.875457363412258E-4	0.0	0.011981867440606548	0.0	0.0
11	8.875457363412258E-4	0.0	0.01686336899048329	2.2188643408530646E-4	0.0
12	8.875457363412258E-4	0.0	0.01752902829273921	2.2188643408530646E-4	0.0
13	8.875457363412258E-4	0.0	0.018860346897251048	2.2188643408530646E-4	0.0
14	8.875457363412258E-4	0.0	0.01952600619950697	2.2188643408530646E-4	0.0
15	8.875457363412258E-4	0.0	0.021744870540360034	2.2188643408530646E-4	0.0
16	8.875457363412258E-4	0.0	0.022854302710786565	2.2188643408530646E-4	0.0
17	8.875457363412258E-4	0.0	0.022854302710786565	4.437728681706129E-4	0.0
18	8.875457363412258E-4	0.0	0.02329807557895718	4.437728681706129E-4	0.0
19	8.875457363412258E-4	0.0	0.02440750774938371	6.656593022559194E-4	0.0
20	8.875457363412258E-4	0.0	0.02507316705163963	6.656593022559194E-4	0.0
21	8.875457363412258E-4	0.0	0.025960712787980855	0.0013313186045118388	0.0
22	8.875457363412258E-4	0.0	0.02640448565615147	0.0015532050385971452	0.0
23	8.875457363412258E-4	0.0	0.026626372090236775	0.0022188643408530646	0.0
24	8.875457363412258E-4	0.0	0.026626372090236775	0.002884523643108984	0.0
25	8.875457363412258E-4	0.0	0.026626372090236775	0.0031064100771942904	0.0
26	8.875457363412258E-4	0.0	0.026626372090236775	0.004215842247620822	0.0
27	8.875457363412258E-4	0.0	0.026626372090236775	0.005990933720303274	0.0
28	8.875457363412258E-4	0.0	0.026626372090236775	0.016641482556397986	0.0
29	8.875457363412258E-4	0.0	0.026626372090236775	0.033948624415051885	0.0
30	8.875457363412258E-4	0.0	0.026626372090236775	0.06101876937345928	0.0
31	8.875457363412258E-4	0.0	0.026626372090236775	0.1477763651008141	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACCT	40	0.0019293756	23.125	33
GGTATCA	350	0.0	21.671429	1
GTATCAA	965	0.0	21.663212	1
TATACTG	45	0.0038222533	20.555557	5
GTATTAG	195	0.0	19.923077	1
CGAGTCG	65	6.890234E-5	19.923077	21
TATTAGC	95	1.6704689E-7	19.473684	2
TTAGCTA	50	0.0070284083	18.5	4
AGGACCG	50	0.0070284083	18.5	5
GCCGTCC	70	1.21711135E-4	18.5	27
GCGTAAT	50	0.0070284083	18.5	1
TGTGCAA	125	8.536517E-9	17.76	10
TTAACGG	115	6.379378E-8	17.69565	35
GAGTCGC	75	2.0637552E-4	17.266666	22
TTTCGGG	65	0.0015779193	17.076923	22
CGCCGTC	80	3.3768604E-4	16.1875	26
TCCGCCG	70	0.0025892158	15.857142	31
CGCGCGA	70	0.0025892158	15.857142	36
GTGTACA	70	0.0025892158	15.857142	1
TCGCCGT	95	7.044463E-5	15.578948	25
>>END_MODULE