FastQCFastQC Report
Fri 10 Feb 2017
ERR1632891.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632891.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257690
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41841.6236563312507275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33211.2887578097714307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17940.6961853389731848No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8910.34576429042648144No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG8530.3310178897124452No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7190.27901742403663315No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7110.2759129186231518No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG6880.26698746555939307No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6150.23865885366137607No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6070.23555434824789478No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4920.19092708292910085No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4690.18200162986534207No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4650.1804493771586014No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4350.16880748185804648No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4090.15871783926423222No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT3690.14319531219682563No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3680.14280724902014047No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3420.1327176064263262No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3410.13232954324964102No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3380.13116535371958557No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3350.13000116418953006No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3300.12806084830610423No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3290.12767278512941907No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3260.1265085955993636No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3260.1265085955993636No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3170.1230160270091971No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3080.11952345841903063No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3050.11835926888897512No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3010.11680701618223446No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2980.11564282665217898No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2980.11564282665217898No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA2960.11486670029880865No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT2940.11409057394543831No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG2930.11370251076875315No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT2870.11137413170864217No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2870.11137413170864217No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2690.10438899452830921No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2670.10361286817493888No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2670.10361286817493888No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2610.1012844891148279No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC2580.10012029958477241No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2580.10012029958477241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG303.5917992E-430.83333423
TTAGCGG303.5917992E-430.83333424
CGCGAAA250.005488530329.632
GCATTAG250.005488530329.621
CACTAGT250.005488530329.64
GGTATCA6750.029.0518511
GCTGAAC358.850651E-426.4285723
CCGCGAA358.850651E-426.42857231
GCTCTCT1850.023.030
GTATCAA17400.022.2212661
GCTTCGC2200.021.86363622
GCCGCTC2050.021.65853727
GGACCGT603.7118614E-521.5833346
CCGCTCT2000.021.27500228
CGCTCTC2000.021.27500229
GAATCAA705.076614E-621.1428591
CCGGCAG2200.021.02272816
AGCTTCG2400.020.812521
CTTCGCC2250.020.55555523
TCGCACC450.003817877520.55555528
Produced by FastQC (version 0.11.5)