FastQCFastQC Report
Fri 10 Feb 2017
ERR1632890.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632890.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131518
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC3020.22962636293130978No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC2980.22658495415076263No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT2690.2045347404917958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2580.19617086634529116No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA2480.18856734439392325No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA2430.18476558341823932No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.15815325658845178No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG2070.157392904393315No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT2060.1566325521981782No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT1970.14978938244194712No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC1930.14674797366139997No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA1900.1444669170759896No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA1870.14218586049057924No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC1860.14142550829544245No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG1840.13990480390516888No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT1770.13458233853921137No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG1690.12849952097811707No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG1670.1269788165878435No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA1510.11481318146565489No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC1420.1079700117094238No Hit
GGAAATAACTATATCACTATTCAAGATCATCTTCACAACATCA1380.10492860292887665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1360.10340789853860308No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA1350.1026475463434663No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA1350.1026475463434663No Hit
GTGGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAA1330.10112684195319273No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA1320.10036648975805594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA355.580896E-737.01
GTGTATA352.3723756E-531.7142871
TTGGCCG706.4792403E-926.42857231
GTATCAA1500.025.91
GGCCGCA751.3502358E-824.66666633
TGGCCGC751.3502358E-824.66666632
ACGGGAG451.314527E-424.66666616
GCCGCAG751.3502358E-824.66666634
AACTTGG851.9372237E-923.94117728
TTGCATG652.6529706E-622.7692325
TGTATAA502.6845097E-422.22
GCATTAC502.6845097E-422.212
GCCAATA705.045462E-621.14285931
GGCAACG450.003808087220.55555519
GCAGACA909.3192284E-820.55555537
CATTACA555.109882E-420.18181813
TGGTACA555.109882E-420.1818184
TAATCCA555.109882E-420.18181822
TGCCAAT656.842911E-519.92307730
AGGCCAC656.842911E-519.92307712
Produced by FastQC (version 0.11.5)