FastQCFastQC Report
Fri 10 Feb 2017
ERR1632889.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632889.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338818
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12680.3742422185362053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9380.2768447957310414No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA5150.15199900831714963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5020.1481621401460371No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGG250.00549094129.610
GGTATCA2400.028.5208341
CTTTCGA358.8564376E-426.4285711
CGAACTA659.351061E-825.61538529
GCGAACT701.9146137E-723.78571328
CCGGCTA400.001928392423.12525
GTATCAA7050.022.5673751
AGCGAAC753.7246718E-722.227
GTATTAT705.084419E-621.1428571
TTGTACT705.084419E-621.1428574
CAAAGCG806.9303496E-720.812524
GCCTTAA450.003820324320.5555551
GCGACTA450.003820324320.55555515
ATAGGGG450.003820324320.5555553
CACGCCC450.003820324320.55555520
TCAGCGG450.003820324320.55555526
ACCACGC450.003820324320.55555518
GACTATG555.1328103E-420.1818187
CGGCAAG500.007024895418.518
CCCGTCA500.007024895418.523
Produced by FastQC (version 0.11.5)