##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632889.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	338818
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.564969983885153	33.0	33.0	33.0	27.0	33.0
2	31.132900259136175	33.0	33.0	33.0	27.0	33.0
3	31.54815269554746	33.0	33.0	33.0	27.0	33.0
4	31.700204829731597	33.0	33.0	33.0	27.0	33.0
5	31.808918062204487	33.0	33.0	33.0	33.0	33.0
6	34.64212645136917	37.0	37.0	37.0	27.0	37.0
7	34.764062121847125	37.0	37.0	37.0	27.0	37.0
8	34.975320083348585	37.0	37.0	37.0	27.0	37.0
9	35.029434681746544	37.0	37.0	37.0	27.0	37.0
10	35.09024609082162	37.0	37.0	37.0	33.0	37.0
11	35.13391260204594	37.0	37.0	37.0	33.0	37.0
12	35.06176472324375	37.0	37.0	37.0	33.0	37.0
13	35.10207249909981	37.0	37.0	37.0	33.0	37.0
14	35.069789680595484	37.0	37.0	37.0	33.0	37.0
15	35.10356002337538	37.0	37.0	37.0	33.0	37.0
16	35.077599183042224	37.0	37.0	37.0	33.0	37.0
17	35.08717069341062	37.0	37.0	37.0	33.0	37.0
18	35.05794851513202	37.0	37.0	37.0	27.0	37.0
19	35.089168816296656	37.0	37.0	37.0	33.0	37.0
20	35.016687425107285	37.0	37.0	37.0	27.0	37.0
21	35.07058656860025	37.0	37.0	37.0	33.0	37.0
22	35.08588386685477	37.0	37.0	37.0	33.0	37.0
23	35.05685353198472	37.0	37.0	37.0	33.0	37.0
24	35.03102255488197	37.0	37.0	37.0	27.0	37.0
25	35.051850846177004	37.0	37.0	37.0	27.0	37.0
26	35.02016421795772	37.0	37.0	37.0	27.0	37.0
27	34.93129644824065	37.0	37.0	37.0	27.0	37.0
28	34.92950492594844	37.0	37.0	37.0	27.0	37.0
29	34.97900347679285	37.0	37.0	37.0	27.0	37.0
30	34.951805984333774	37.0	37.0	37.0	27.0	37.0
31	34.911350636624974	37.0	37.0	37.0	27.0	37.0
32	34.954810547255455	37.0	37.0	37.0	27.0	37.0
33	34.923714206447116	37.0	37.0	37.0	27.0	37.0
34	34.85748395893961	37.0	37.0	37.0	27.0	37.0
35	34.87288455749104	37.0	37.0	37.0	27.0	37.0
36	34.83433583811958	37.0	37.0	37.0	27.0	37.0
37	34.87032271012756	37.0	37.0	37.0	27.0	37.0
38	34.71674173154909	37.0	37.0	37.0	27.0	37.0
39	34.76561752917495	37.0	37.0	37.0	27.0	37.0
40	34.81079222473422	37.0	37.0	37.0	27.0	37.0
41	34.833816385197956	37.0	37.0	37.0	27.0	37.0
42	34.71392606059891	37.0	37.0	37.0	27.0	37.0
43	34.51992810299335	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	11.0
16	17.0
17	15.0
18	11.0
19	21.0
20	33.0
21	101.0
22	264.0
23	640.0
24	1224.0
25	2102.0
26	3269.0
27	4815.0
28	6791.0
29	8844.0
30	11430.0
31	14524.0
32	18011.0
33	23265.0
34	32465.0
35	58340.0
36	152622.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.76288744989935	21.22319357295067	13.664563275859015	24.34935570129096
2	15.617824318660755	23.78887780460306	38.60509181920677	21.98820605752941
3	17.75879675814154	27.260062924638007	31.205543979363554	23.775596337856904
4	12.641595192699326	17.99136999805205	39.96718001995171	29.39985478929691
5	13.328099451622997	37.71051124792662	35.843432167122174	13.117957133328218
6	28.72043397930452	40.680542356073175	17.506448889964524	13.09257477465778
7	25.325100791575416	33.013004031663016	24.179352926940126	17.482542249821435
8	24.081955504134964	36.47828627758856	21.216995555135796	18.22276266314068
9	25.687537261892817	15.666523030063336	20.809106954175988	37.83683275386786
10	13.968266148787844	29.20535508739205	34.87211423241977	21.954264531400337
11	32.86307102928416	24.483646087279897	24.626790784432938	18.026492099003004
12	22.03159218223353	26.371090083761782	31.72617747581297	19.871140258191712
13	27.596526748874027	22.864782862775883	27.064677791616738	22.47401259673335
14	21.594484354432172	21.830894462513797	28.682950728709812	27.89167045434422
15	22.898429245199488	29.83637232968733	25.21589762055145	22.049300804561742
16	21.946885938763582	29.64069205296059	25.866984634818692	22.545437373457137
17	21.49767721903795	28.39990791516389	27.289282151479554	22.813132714318602
18	22.15319138888725	27.931219710877226	28.57994557550071	21.335643324734814
19	23.858236575388556	26.971412380688157	28.669078974552708	20.501272069370575
20	23.811308726218797	26.954884333181827	28.390168172883378	20.843638767716
21	22.34119792927176	28.025075409216747	27.805783636052396	21.827943025459096
22	21.751795949447786	27.99939790684084	28.096205042235063	22.152601101476307
23	21.855096246362354	27.503851625356386	28.590570748897637	22.050481379383623
24	22.355364827134334	27.950108908027318	27.85772892821515	21.8367973366232
25	22.271544014780797	27.60331505409984	28.35386549711054	21.771275434008817
26	22.11275670123783	27.66677095077593	28.19478303986211	22.025689308124125
27	22.052252241616443	27.88901416099499	27.822016539853255	22.236717057535312
28	21.661777119279378	27.746754894958354	28.59735905412345	21.994108931638813
29	22.512676422149944	27.806078779757865	28.0067764994776	21.674468298614595
30	22.17798346014675	27.911149938905254	28.183272435348773	21.727594165599232
31	22.26003341026746	27.665590375954054	28.42735627977262	21.64701993400587
32	21.260381679839917	27.80047104935393	28.378362424664573	22.560784846141583
33	21.751795949447786	27.605085916332662	29.029154295226345	21.613963838993204
34	21.873100012396034	27.183325561215753	28.492583038681534	22.450991387706672
35	22.026279595535065	28.12335826313832	28.207179075491855	21.643183065834755
36	22.275380882951907	27.770661535101443	28.160841513733036	21.793116068213614
37	22.342673647799113	27.41737451965362	27.780696421087427	22.45925541145984
38	22.30578068461534	27.76948096027956	28.074954695441214	21.849783659663892
39	22.081766612163463	27.55166490564256	28.380428430602862	21.986140051591118
40	21.945410220236234	27.6750349745291	28.5761087073296	21.80344609790507
41	21.839748773677904	27.18716242938687	28.68442644723716	22.28866234969807
42	22.03247761334994	27.317615947204693	28.307823079057194	22.342083360388173
43	21.93744134018854	26.941307722730194	28.6681935434363	22.453057393644965
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18.0
1	30.0
2	42.0
3	103.0
4	164.0
5	164.0
6	200.0
7	236.0
8	225.0
9	214.0
10	285.5
11	357.0
12	357.0
13	552.0
14	747.0
15	1420.0
16	2093.0
17	2481.0
18	2869.0
19	2869.0
20	3003.0
21	3137.0
22	3850.5
23	4564.0
24	5730.0
25	6896.0
26	6896.0
27	8443.0
28	9990.0
29	12547.5
30	15105.0
31	16540.0
32	17975.0
33	17975.0
34	18996.0
35	20017.0
36	21422.0
37	22827.0
38	23790.5
39	24754.0
40	24754.0
41	25338.0
42	25922.0
43	25364.0
44	24806.0
45	23990.5
46	23175.0
47	23175.0
48	22171.5
49	21168.0
50	21371.0
51	21574.0
52	21463.0
53	21352.0
54	21352.0
55	19593.0
56	17834.0
57	16192.5
58	14551.0
59	12697.5
60	10844.0
61	10844.0
62	9705.0
63	8566.0
64	7079.0
65	5592.0
66	4765.5
67	3939.0
68	3939.0
69	3221.0
70	2503.0
71	2261.5
72	2020.0
73	1617.5
74	1215.0
75	1215.0
76	922.5
77	630.0
78	536.0
79	442.0
80	308.0
81	174.0
82	174.0
83	169.5
84	165.0
85	158.0
86	151.0
87	129.5
88	108.0
89	108.0
90	84.0
91	60.0
92	38.5
93	17.0
94	11.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	338818.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.87995731528288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.00023597305058	67.0497327071188
2	4.423338461282372	6.447454364986166
3	1.1893572835988	2.6004092418390403
4	0.5540512717390076	1.615169321392683
5	0.343804990155133	1.2528246503643676
6	0.22396002920023814	0.979331842106572
7	0.1830343586012534	0.933767536946267
8	0.12767364628256622	0.7443879913087962
9	0.08617298456640078	0.5652255093236838
>10	0.7768899893519782	11.117303409365771
>50	0.06586632876367114	3.2529679099897963
>100	0.023988354302721043	2.486395858320203
>500	0.0012197468289519174	0.5792997143386959
>1k	4.065822763173058E-4	0.3757299425992155
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1268	0.3742422185362053	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	938	0.2768447957310414	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	515	0.15199900831714963	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	502	0.1481621401460371	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.9514370547019343E-4	0.0
5	0.0	0.0	2.9514370547019343E-4	2.9514370547019343E-4	0.0
6	0.0	0.0	5.902874109403869E-4	2.9514370547019343E-4	0.0
7	0.0	0.0	0.0011805748218807737	2.9514370547019343E-4	0.0
8	0.0	0.0	0.0014757185273509671	2.9514370547019343E-4	0.0
9	0.0	0.0	0.0017708622328211606	5.902874109403869E-4	0.0
10	2.9514370547019343E-4	0.0	0.0017708622328211606	5.902874109403869E-4	0.0
11	2.9514370547019343E-4	0.0	0.0029514370547019343	5.902874109403869E-4	0.0
12	2.9514370547019343E-4	0.0	0.0038368681711125146	5.902874109403869E-4	0.0
13	2.9514370547019343E-4	0.0	0.004722299287523095	5.902874109403869E-4	0.0
14	2.9514370547019343E-4	0.0	0.005312586698463482	8.854311164105803E-4	0.0
15	2.9514370547019343E-4	0.0	0.005607730403933676	8.854311164105803E-4	0.0
16	2.9514370547019343E-4	0.0	0.006493161520344255	8.854311164105803E-4	0.0
17	2.9514370547019343E-4	0.0	0.006493161520344255	8.854311164105803E-4	0.0
18	2.9514370547019343E-4	0.0	0.006493161520344255	8.854311164105803E-4	0.0
19	2.9514370547019343E-4	0.0	0.006493161520344255	8.854311164105803E-4	0.0
20	2.9514370547019343E-4	0.0	0.006493161520344255	0.0011805748218807737	0.0
21	2.9514370547019343E-4	0.0	0.006493161520344255	0.0014757185273509671	0.0
22	2.9514370547019343E-4	0.0	0.006788305225814449	0.0014757185273509671	0.0
23	2.9514370547019343E-4	0.0	0.006788305225814449	0.0017708622328211606	0.0
24	2.9514370547019343E-4	0.0	0.006788305225814449	0.0017708622328211606	0.0
25	2.9514370547019343E-4	0.0	0.006788305225814449	0.0017708622328211606	0.0
26	2.9514370547019343E-4	0.0	0.007083448931284642	0.002656293349231741	0.0
27	2.9514370547019343E-4	0.0	0.007083448931284642	0.004427155582052902	0.0
28	2.9514370547019343E-4	0.0	0.007083448931284642	0.01033002969145677	0.0
29	2.9514370547019343E-4	0.0	0.007083448931284642	0.020069771971973155	0.0
30	2.9514370547019343E-4	0.0	0.007083448931284642	0.03836868171112515	0.0
31	2.9514370547019343E-4	0.0	0.007083448931284642	0.09178969240123015	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACCGG	25	0.005490941	29.6	10
GGTATCA	240	0.0	28.520834	1
CTTTCGA	35	8.8564376E-4	26.42857	11
CGAACTA	65	9.351061E-8	25.615385	29
GCGAACT	70	1.9146137E-7	23.785713	28
CCGGCTA	40	0.0019283924	23.125	25
GTATCAA	705	0.0	22.567375	1
AGCGAAC	75	3.7246718E-7	22.2	27
GTATTAT	70	5.084419E-6	21.142857	1
TTGTACT	70	5.084419E-6	21.142857	4
CAAAGCG	80	6.9303496E-7	20.8125	24
GCCTTAA	45	0.0038203243	20.555555	1
GCGACTA	45	0.0038203243	20.555555	15
ATAGGGG	45	0.0038203243	20.555555	3
CACGCCC	45	0.0038203243	20.555555	20
TCAGCGG	45	0.0038203243	20.555555	26
ACCACGC	45	0.0038203243	20.555555	18
GACTATG	55	5.1328103E-4	20.181818	7
CGGCAAG	50	0.0070248954	18.5	18
CCCGTCA	50	0.0070248954	18.5	23
>>END_MODULE