FastQCFastQC Report
Fri 10 Feb 2017
ERR1632888.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632888.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119645
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12691.0606377199214343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11330.9469681140039283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6510.5441096577374733No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC3030.25324919553679637No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2810.23486146516778802No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC2760.23068243553846798No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT2560.21396631702118768No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC2300.19223536294872332No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG2240.18722052739353923No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG2170.18136988591249112No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2130.17802666220903507No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.17134021480212294No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA2040.17050440887625892No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC1980.16548957332107483No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA1970.16465376739521084No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG1820.1521166785072506No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG1820.1521166785072506No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG1780.14877345480379456No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1750.14626603702620253No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA1730.1445944251744745No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC1660.13874378369342638No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG1640.13707217184169834No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT1620.13540055998997033No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC1590.13289314221237827No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC1570.13122153036065026No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT1530.1278783066571942No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT1500.12537088887960215No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG1470.12286347110201012No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT1470.12286347110201012No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG1460.12202766517614609No Hit
ACCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGA1450.12119185925028209No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT1440.12035605332441808No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC1440.12035605332441808No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC1410.11784863554682604No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG1370.11450541184336996No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA1330.11116218813991391No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1320.1103263822140499No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT1300.10865477036232186No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1290.10781896443645786No Hit
GATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGAT1260.10531154665886582No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA1250.1044757407330018No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA1240.10363993480713779No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG1240.10363993480713779No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG1240.10363993480713779No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT1200.10029671110368174No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTGA200.001835351237.015
GGAGAGT200.001835351237.06
CGTGGTT250.00547691829.631
AACGTGG250.00547691829.629
GAGTGAT250.00547691829.69
CGAGTCG250.00547691829.621
AGAACGT250.00547691829.627
GTTACTA250.00547691829.635
TGGTTAC250.00547691829.633
GAGAACG250.00547691829.626
GAACGTG250.00547691829.628
CCTGGGC250.00547691829.637
CAGAGTT250.00547691829.622
CCTATAG405.89642E-527.7510
TTGTCCG556.1832543E-726.9090913
TGTCCGG556.1832543E-726.9090914
GGTATCA2650.026.5283031
CTGCTCG1051.8189894E-1224.6666669
CTATAGA451.3135537E-424.66666611
TGCTCGC451.3135537E-424.66666610
Produced by FastQC (version 0.11.5)