##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632888.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	119645
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.91094487859919	33.0	33.0	33.0	14.0	33.0
2	29.882895231727193	33.0	33.0	33.0	14.0	33.0
3	30.35673868527728	33.0	33.0	33.0	14.0	33.0
4	30.509172970036357	33.0	33.0	33.0	27.0	33.0
5	30.677303690083164	33.0	33.0	33.0	27.0	33.0
6	32.16983576413557	37.0	33.0	37.0	14.0	37.0
7	32.5260980400351	37.0	33.0	37.0	14.0	37.0
8	32.74024823435998	37.0	33.0	37.0	14.0	37.0
9	32.51382840904342	37.0	33.0	37.0	14.0	37.0
10	32.471001713402146	37.0	33.0	37.0	14.0	37.0
11	32.57582849262401	37.0	33.0	37.0	14.0	37.0
12	32.65606586150696	37.0	33.0	37.0	14.0	37.0
13	32.74405950938192	37.0	33.0	37.0	14.0	37.0
14	32.70459275356262	37.0	33.0	37.0	14.0	37.0
15	32.80356053324418	37.0	33.0	37.0	14.0	37.0
16	32.825433574324045	37.0	33.0	37.0	14.0	37.0
17	32.89072673325254	37.0	33.0	37.0	14.0	37.0
18	32.87873291821639	37.0	33.0	37.0	14.0	37.0
19	32.83367462075306	37.0	33.0	37.0	14.0	37.0
20	32.84487442015964	37.0	33.0	37.0	14.0	37.0
21	32.9143382506582	37.0	33.0	37.0	14.0	37.0
22	32.87054202014292	37.0	33.0	37.0	14.0	37.0
23	32.844222491537465	37.0	33.0	37.0	14.0	37.0
24	32.864331982113754	37.0	33.0	37.0	14.0	37.0
25	32.7808015378829	37.0	33.0	37.0	14.0	37.0
26	32.76571524092106	37.0	33.0	37.0	14.0	37.0
27	32.65139370638138	37.0	33.0	37.0	14.0	37.0
28	32.61156755401396	37.0	33.0	37.0	14.0	37.0
29	32.63326507584939	37.0	33.0	37.0	14.0	37.0
30	32.57533536712775	37.0	33.0	37.0	14.0	37.0
31	32.52359898031677	37.0	33.0	37.0	14.0	37.0
32	32.53699694930837	37.0	33.0	37.0	14.0	37.0
33	32.4598353462326	37.0	33.0	37.0	14.0	37.0
34	32.3444690542856	37.0	33.0	37.0	14.0	37.0
35	32.3149233148063	37.0	33.0	37.0	14.0	37.0
36	32.28916377617117	37.0	33.0	37.0	14.0	37.0
37	32.31835011910234	37.0	33.0	37.0	14.0	37.0
38	32.166183292239545	37.0	27.0	37.0	14.0	37.0
39	32.2351623553011	37.0	33.0	37.0	14.0	37.0
40	32.169727109365205	37.0	27.0	37.0	14.0	37.0
41	32.23542981319738	37.0	33.0	37.0	14.0	37.0
42	32.014024823435996	37.0	27.0	37.0	14.0	37.0
43	31.644665468678173	37.0	27.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	9.0
16	22.0
17	19.0
18	14.0
19	64.0
20	165.0
21	373.0
22	758.0
23	1230.0
24	1940.0
25	2895.0
26	3819.0
27	4898.0
28	6034.0
29	7186.0
30	8260.0
31	9749.0
32	11130.0
33	12648.0
34	15161.0
35	17954.0
36	15316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.85716076726984	22.851769819048016	12.503656650925654	22.78741276275649
2	17.938902586819342	25.854820510677424	34.159388190062266	22.04688871244097
3	19.08562831710477	29.340967027456227	29.77140707927619	21.801997576162815
4	13.317731622717204	22.21739312131723	37.71156337498433	26.753311880981233
5	14.973463161853818	38.325044924568516	33.904467382673744	12.797024530903926
6	26.541017175811778	42.814158552384136	16.45869029211417	14.186133979689917
7	26.24848510175937	33.52584729825735	23.024781645701868	17.200885954281418
8	24.72564670483514	35.33954615738226	19.45672614818839	20.478080989594215
9	27.04166492540432	14.112583058213882	20.313427222199003	38.53232479418279
10	16.646746625433575	27.88164988089766	32.66496719461741	22.806636299051362
11	34.16022399598813	24.489949433741483	22.977140707927617	18.372685862342763
12	23.409252371599315	28.42743115048686	27.804755735718167	20.35856074219566
13	28.86205023193614	23.165197041247023	25.54473651218187	22.42801621463496
14	22.204020226503406	21.909816540599273	28.826946383049858	27.059216849847466
15	25.27644280997952	28.13072004680513	26.116427765472856	20.47640937774249
16	21.791132099126585	28.37393957123156	26.571106189142878	23.263822140498977
17	22.17309540724644	27.454553052781144	26.172426762505747	24.199924777466673
18	22.36533077019516	26.466630448409877	29.27744577709056	21.8905930043044
19	25.64921225291487	25.187847381837937	29.582514940030926	19.580425425216262
20	23.971749759705794	25.214593171465587	30.403276359229388	20.41038070959923
21	21.79614693468177	27.666847757950602	29.595887834844746	20.94111747252288
22	21.993397133185674	27.066739103180243	29.05512140081073	21.884742362823353
23	21.820385306531822	26.91044339504367	31.00338501399975	20.265786284424756
24	21.77358017468344	28.03710978310836	27.80559154164403	22.38371850056417
25	22.38789753019349	26.85444439801078	30.405783777006977	20.35187429478875
26	21.85465334949225	27.77466672238706	29.91015086296962	20.46052906515107
27	22.626102219064734	26.931338543190268	29.040076894145177	21.402482343599814
28	21.227798905094236	27.043336537256053	30.617242676250573	21.111621881399138
29	23.542145513811693	27.091813280956163	28.281165113460656	21.08487609177149
30	22.830038864975553	26.144845166952237	30.07647624221656	20.948639725855656
31	22.48150779389026	27.058381043921603	29.521501107442848	20.93861005474529
32	20.53742321033056	26.98483012244557	29.880061849638512	22.597684817585357
33	20.62267541476869	27.024113000961176	30.517781771072755	21.835429813197376
34	21.910652346525136	26.653850975803415	30.041372393330267	21.394124284341174
35	21.496092607296585	27.846546032011364	29.60257428225166	21.054787078440388
36	22.657862844247564	27.76881608090601	28.183375820134565	21.389945254711858
37	21.771072756905845	27.151991307618374	29.16711939487651	21.909816540599273
38	22.015963893184004	27.176229679468427	28.680680346023653	22.127126081323915
39	21.016340005850644	27.33837602908605	29.296669313385433	22.34861465167788
40	21.30970788582891	28.256090935684735	29.788959003719334	20.64524217476702
41	21.837101425049106	26.662209035062055	28.77178319194283	22.72890634794601
42	20.922729742153873	28.56617493418028	28.61047264825108	21.90062267541477
43	21.944084583559697	26.795937983200304	28.572861381587195	22.687116051652804
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	17.5
2	10.0
3	42.0
4	74.0
5	74.0
6	129.0
7	184.0
8	191.0
9	198.0
10	285.5
11	373.0
12	373.0
13	590.0
14	807.0
15	1480.5
16	2154.0
17	2143.0
18	2132.0
19	2132.0
20	1717.0
21	1302.0
22	960.5
23	619.0
24	649.5
25	680.0
26	680.0
27	853.5
28	1027.0
29	1545.0
30	2063.0
31	2906.5
32	3750.0
33	3750.0
34	4895.0
35	6040.0
36	6739.5
37	7439.0
38	8089.0
39	8739.0
40	8739.0
41	9668.0
42	10597.0
43	11145.0
44	11693.0
45	11249.0
46	10805.0
47	10805.0
48	10605.5
49	10406.0
50	10566.0
51	10726.0
52	9688.0
53	8650.0
54	8650.0
55	7829.0
56	7008.0
57	5589.5
58	4171.0
59	3732.0
60	3293.0
61	3293.0
62	2715.5
63	2138.0
64	1560.5
65	983.0
66	875.0
67	767.0
68	767.0
69	563.0
70	359.0
71	269.5
72	180.0
73	188.5
74	197.0
75	197.0
76	118.5
77	40.0
78	25.0
79	10.0
80	5.5
81	1.0
82	1.0
83	1.5
84	2.0
85	1.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	119645.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.35254293952944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.24729847795099	27.71783191942831
2	8.564399687313193	6.226754147686907
3	3.6947624959764567	4.029420368590413
4	2.278475191980503	3.3131346901249534
5	1.5841265461902792	2.8793514146015298
6	1.1564813537499425	2.5224622842575957
7	0.9380604221271899	2.387061724267625
8	0.7311353290108981	2.1262902753980524
9	0.521911068193314	1.7075515065401816
>10	3.7315491791971307	26.747461239500186
>50	0.4092518508299996	10.229428726649672
>100	0.1356508943762358	7.561536211291737
>500	0.0022991677012921324	0.5441096577374733
>1k	0.004598335402584265	2.0076058339253624
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1269	1.0606377199214343	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1133	0.9469681140039283	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	651	0.5441096577374733	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	303	0.25324919553679637	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	281	0.23486146516778802	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	276	0.23068243553846798	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	256	0.21396631702118768	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	230	0.19223536294872332	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	224	0.18722052739353923	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	217	0.18136988591249112	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	213	0.17802666220903507	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	205	0.17134021480212294	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	204	0.17050440887625892	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	198	0.16548957332107483	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	197	0.16465376739521084	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	182	0.1521166785072506	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	182	0.1521166785072506	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	178	0.14877345480379456	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	175	0.14626603702620253	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	173	0.1445944251744745	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	166	0.13874378369342638	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	164	0.13707217184169834	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	162	0.13540055998997033	No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC	159	0.13289314221237827	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	157	0.13122153036065026	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT	153	0.1278783066571942	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	150	0.12537088887960215	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	147	0.12286347110201012	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	147	0.12286347110201012	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	146	0.12202766517614609	No Hit
ACCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGA	145	0.12119185925028209	No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT	144	0.12035605332441808	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	144	0.12035605332441808	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	141	0.11784863554682604	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	137	0.11450541184336996	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	133	0.11116218813991391	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	132	0.1103263822140499	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	130	0.10865477036232186	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	129	0.10781896443645786	No Hit
GATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGAT	126	0.10531154665886582	No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA	125	0.1044757407330018	No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA	124	0.10363993480713779	No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG	124	0.10363993480713779	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	124	0.10363993480713779	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	120	0.10029671110368174	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0025074177775920433	0.0	0.0
2	8.358059258640144E-4	0.0	0.0033432237034560575	0.0	0.0
3	8.358059258640144E-4	0.0	0.005850641481048101	0.0	0.0
4	8.358059258640144E-4	0.0	0.008358059258640143	0.0	0.0
5	8.358059258640144E-4	0.0	0.010029671110368173	0.0	0.0
6	0.0016716118517280287	0.0	0.012537088887960216	0.0	0.0
7	0.0016716118517280287	0.0	0.023402565924192403	8.358059258640144E-4	0.0
8	0.0016716118517280287	0.0	0.029253207405240504	8.358059258640144E-4	0.0
9	0.0016716118517280287	0.0	0.03761126666388065	8.358059258640144E-4	0.0
10	0.004179029629320072	0.0	0.04346190814492875	8.358059258640144E-4	0.0
11	0.004179029629320072	0.0	0.05934222073634502	8.358059258640144E-4	0.0
12	0.004179029629320072	0.0	0.0635212503656651	8.358059258640144E-4	0.0
13	0.004179029629320072	0.0	0.06937189184671319	8.358059258640144E-4	0.0
14	0.004179029629320072	0.0	0.07187930962430523	0.0016716118517280287	0.0
15	0.004179029629320072	0.0	0.08023736888294539	0.0016716118517280287	0.0
16	0.004179029629320072	0.0	0.09110284591917757	0.0016716118517280287	0.0
17	0.004179029629320072	0.0	0.09444606962263362	0.0016716118517280287	0.0
18	0.004179029629320072	0.0	0.09611768147436166	0.0016716118517280287	0.0
19	0.004179029629320072	0.0	0.09946090517781771	0.0016716118517280287	0.0
20	0.004179029629320072	0.0	0.10113251702954575	0.0016716118517280287	0.0
21	0.004179029629320072	0.0	0.10363993480713778	0.0016716118517280287	0.0
22	0.004179029629320072	0.0	0.10614735258472982	0.005014835555184087	0.0
23	0.004179029629320072	0.0	0.10698315851059384	0.007522253332776129	0.0
24	0.004179029629320072	0.0	0.10865477036232186	0.007522253332776129	0.0
25	0.004179029629320072	0.0	0.10865477036232186	0.007522253332776129	0.0
26	0.004179029629320072	0.0	0.10865477036232186	0.007522253332776129	0.0
27	0.004179029629320072	0.0	0.10865477036232186	0.010029671110368173	0.0
28	0.004179029629320072	0.0	0.10865477036232186	0.023402565924192403	0.0
29	0.004179029629320072	0.0	0.10865477036232186	0.05683480295875298	0.0
30	0.004179029629320072	0.0	0.10865477036232186	0.08943123406744954	0.0
31	0.004179029629320072	0.0	0.10865477036232186	0.19975761628149943	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTGA	20	0.0018353512	37.0	15
GGAGAGT	20	0.0018353512	37.0	6
CGTGGTT	25	0.005476918	29.6	31
AACGTGG	25	0.005476918	29.6	29
GAGTGAT	25	0.005476918	29.6	9
CGAGTCG	25	0.005476918	29.6	21
AGAACGT	25	0.005476918	29.6	27
GTTACTA	25	0.005476918	29.6	35
TGGTTAC	25	0.005476918	29.6	33
GAGAACG	25	0.005476918	29.6	26
GAACGTG	25	0.005476918	29.6	28
CCTGGGC	25	0.005476918	29.6	37
CAGAGTT	25	0.005476918	29.6	22
CCTATAG	40	5.89642E-5	27.75	10
TTGTCCG	55	6.1832543E-7	26.90909	13
TGTCCGG	55	6.1832543E-7	26.90909	14
GGTATCA	265	0.0	26.528303	1
CTGCTCG	105	1.8189894E-12	24.666666	9
CTATAGA	45	1.3135537E-4	24.666666	11
TGCTCGC	45	1.3135537E-4	24.666666	10
>>END_MODULE