FastQCFastQC Report
Fri 10 Feb 2017
ERR1632887.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632887.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130450
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44153.3844384821770794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37562.87926408585665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20881.600613261786125No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8940.685320045994634No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG5950.45611345343043314No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.41471828286699886No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG4950.3794557301648141No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4030.3089306247604446No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3970.3043311613645075No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3840.294365657339977No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3750.2874664622460713No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3080.23610578765810658No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2970.22767343809888843No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.21387504791107706No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2680.20544269835185894No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2630.201609812188578No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2470.18934457646607897No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2310.17707934074357992No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2260.17324645458029897No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2210.16941356841701802No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2200.16864699118436183No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.16174779609045611No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.16098121885779992No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2090.16021464162514373No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2050.15714833269451897No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2010.1540820237638942No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.15024913760061326No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1940.14871598313530088No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG1930.1479494059026447No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT1840.14105021080873897No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1820.13951705634342662No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA1800.1379839018781142No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1790.13721732464545802No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT1740.1333844384821771No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC1740.1333844384821771No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT1730.1326178612495209No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1720.13185128401686472No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1690.12955155231889612No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG1660.12725182062092755No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1630.124952088922959No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1620.12418551169030279No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG1610.1234189344576466No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1600.12265235722499042No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT1580.12111920275967804No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA1580.12111920275967804No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1580.12111920275967804No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1550.11881947106170948No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG1540.11805289382905329No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1530.11728631659639707No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1530.11728631659639707No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.11498658489842851No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1470.11268685320045994No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1450.11115369873514758No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC1420.10885396703717899No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1390.10655423533921042No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT1380.10578765810655424No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC1380.10578765810655424No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.10118819471061709No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1310.1004216174779609No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCAA251.2276358E-436.9999961
TGAAATT303.5821655E-430.83333214
AACTCGC250.005478720629.59999822
CCACCCG250.005478720629.59999824
GACCGTT250.005478720629.5999987
TGAACTC250.005478720629.59999820
GGTATCA6350.025.3464571
GTACTGG1150.024.1304361
CTGGTTC1200.023.1249984
TACTGGT1200.023.1249982
ACTGGTT1200.023.1249983
TGCGGGT652.6526995E-622.7692321
ACTAACA753.6884376E-722.233
TGGTTCA1250.022.1999995
GTATCAA15700.022.1528661
TTCTGCG705.0449544E-621.14285718
CTGCGGG705.0449544E-621.14285720
TTCTTCT705.0449544E-621.14285715
TCTTCTG705.0449544E-621.14285716
CTTCTGC705.0449544E-621.14285717
Produced by FastQC (version 0.11.5)