FastQCFastQC Report
Fri 10 Feb 2017
ERR1632886.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632886.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352451
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14960.42445616553790455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12230.34699858987490456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6200.17591097769619038No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG4170.11831431886985709No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4170.11831431886985709No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3940.11178858905209518No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA3900.11065367951857137No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC3570.10129067586699995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7500.022.6933351
TTCGCCG603.7168575E-521.58333424
GGTATCA3450.021.4492741
TGGGGCG450.003820626520.55555337
ACGGTAC450.003820626520.5555533
ACGGGTC555.1333755E-420.18181816
GCCGCTC656.884789E-519.92307727
GTCGCCC851.2403016E-619.58823637
GAGTTAG1205.136826E-918.526
CGGAGTT1205.136826E-918.524
TCACGGT500.00702544518.4999981
CACGGTA500.00702544518.4999982
CGCGGTA500.00702544518.49999812
TTCCGAT500.00702544518.49999816
CTTGGAC500.00702544518.4999983
AATAGCG1451.5279511E-1017.8620686
CGTATTA1258.518327E-917.762
ATTACCG1258.518327E-917.765
GTTAGCC1258.518327E-917.7628
AGTTAGC1258.518327E-917.7627