##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632886.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	352451
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.168763884908824	33.0	33.0	33.0	27.0	33.0
2	30.79308329384794	33.0	33.0	33.0	27.0	33.0
3	31.249716981935077	33.0	33.0	33.0	27.0	33.0
4	31.42722250752587	33.0	33.0	33.0	27.0	33.0
5	31.525925589656435	33.0	33.0	33.0	27.0	33.0
6	34.279048718829	37.0	33.0	37.0	27.0	37.0
7	34.48409849879842	37.0	37.0	37.0	27.0	37.0
8	34.67944763953003	37.0	37.0	37.0	27.0	37.0
9	34.765221264799926	37.0	37.0	37.0	27.0	37.0
10	34.78957926066318	37.0	37.0	37.0	27.0	37.0
11	34.817398730603685	37.0	37.0	37.0	27.0	37.0
12	34.77498999860973	37.0	37.0	37.0	27.0	37.0
13	34.812606575098386	37.0	37.0	37.0	27.0	37.0
14	34.792722960071046	37.0	37.0	37.0	27.0	37.0
15	34.77473180669086	37.0	37.0	37.0	27.0	37.0
16	34.76476730098652	37.0	37.0	37.0	27.0	37.0
17	34.74684140490451	37.0	37.0	37.0	27.0	37.0
18	34.77290460234189	37.0	37.0	37.0	27.0	37.0
19	34.77690799572139	37.0	37.0	37.0	27.0	37.0
20	34.78877063762055	37.0	37.0	37.0	27.0	37.0
21	34.77805425435025	37.0	37.0	37.0	27.0	37.0
22	34.76520707843076	37.0	37.0	37.0	27.0	37.0
23	34.75536173822744	37.0	37.0	37.0	27.0	37.0
24	34.73160808169079	37.0	37.0	37.0	27.0	37.0
25	34.73478299111082	37.0	37.0	37.0	27.0	37.0
26	34.68424263230917	37.0	37.0	37.0	27.0	37.0
27	34.621714791559675	37.0	37.0	37.0	27.0	37.0
28	34.58107368116419	37.0	37.0	37.0	27.0	37.0
29	34.62126933956777	37.0	37.0	37.0	27.0	37.0
30	34.57070911984928	37.0	37.0	37.0	27.0	37.0
31	34.54351101287838	37.0	37.0	37.0	27.0	37.0
32	34.56897270826299	37.0	37.0	37.0	27.0	37.0
33	34.55728597734153	37.0	37.0	37.0	27.0	37.0
34	34.49451696831616	37.0	37.0	37.0	27.0	37.0
35	34.47908503593408	37.0	37.0	37.0	27.0	37.0
36	34.44027680443523	37.0	37.0	37.0	27.0	37.0
37	34.46947235218513	37.0	37.0	37.0	27.0	37.0
38	34.323818062652684	37.0	37.0	37.0	27.0	37.0
39	34.38248153644053	37.0	37.0	37.0	27.0	37.0
40	34.39842701538654	37.0	37.0	37.0	27.0	37.0
41	34.415618057545586	37.0	37.0	37.0	27.0	37.0
42	34.31867692246582	37.0	37.0	37.0	27.0	37.0
43	34.044953766622875	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	17.0
16	22.0
17	18.0
18	24.0
19	25.0
20	54.0
21	191.0
22	426.0
23	912.0
24	1689.0
25	2886.0
26	4459.0
27	6442.0
28	8494.0
29	10982.0
30	14099.0
31	17202.0
32	21413.0
33	26987.0
34	36888.0
35	62414.0
36	136806.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.10386975778193	20.31856910606013	13.290074364947186	25.287486771210748
2	16.859648575262945	23.263091890787653	36.344342901566456	23.532916632382943
3	17.956822366797088	26.750668887306322	29.9936728793506	25.29883586654599
4	12.987337246879708	18.001935020754658	38.61160842216365	30.399119310201982
5	14.63238861572247	36.334412443148125	35.00685201630865	14.026346924820754
6	29.81095244445327	39.66139974067317	16.97767916674942	13.549968648124135
7	27.08773701876289	31.959903646180603	23.18081094960718	17.771548385449325
8	25.140515986619416	35.06813713111894	20.451069794099038	19.340277088162612
9	26.367920647125416	14.718641740270277	19.879075389203038	39.03436222340127
10	15.357028352877421	27.68867161676375	33.700571143222746	23.253728887136084
11	34.6828353444876	23.182229586524084	23.673361687156515	18.4615733818318
12	23.335442373549796	25.5175896791327	29.736332142624082	21.410635804693417
13	28.41586490036913	21.983481391739563	25.65491373268908	23.945739975202226
14	22.41730056092904	20.44851624764861	27.60837676726694	29.525806424155416
15	24.430913800783653	28.06092194375956	24.454463173604275	23.053701081852513
16	23.26167325387075	27.88444351129661	25.006312934280228	23.847570300552416
17	22.956098861969465	26.935375413887318	26.03652706333646	24.071998660806752
18	23.231881878615752	26.630368476752796	27.557305838258365	22.580443806373086
19	25.005178024746705	25.640159908753272	27.121784304768603	22.232877761731416
20	24.494468734660988	25.729817761901653	27.34621266502294	22.42950083841442
21	23.31529772932975	26.586390732328752	27.246908080839603	22.851403457501892
22	23.15470803033613	26.307486714465274	27.092560384280368	23.445244870918227
23	23.137400659949893	26.460415774107602	27.620293317368937	22.78189024857356
24	23.307353362595084	26.89905830881456	26.29500270959651	23.498585618993843
25	23.702018152877987	26.34238518262113	27.530068009453796	22.425528655047085
26	23.463970878221367	26.604833012248513	27.18023214574508	22.750963963785036
27	23.461417331770942	26.5171612507838	26.78329753639513	23.238123881050132
28	22.850552275351752	26.333305906352937	27.67136424637751	23.1447775719178
29	24.19768989164451	26.188604940828654	26.81847973193437	22.795225435592464
30	23.766707996288847	25.976660585443085	27.34904993885675	22.907581479411323
31	23.42822122791537	26.144343469021226	27.115542302334227	23.311893000729178
32	22.621868004346705	26.24705278180513	27.2812390942287	23.849840119619465
33	22.73110304694837	26.241945688904273	27.86855477782727	23.158396486320086
34	23.389918031158942	25.74740885967127	27.705127804999847	23.15754530416994
35	23.24663570255156	26.53191507471961	27.108165390366317	23.113283832362512
36	23.80302510136161	26.446513132321943	26.728821878785986	23.021639887530466
37	23.244082156101133	25.845578534321085	27.102774570082083	23.807564739495703
38	23.377434026290178	26.249038873488793	26.940198779404795	23.433328320816226
39	22.85594309563599	26.196833034946703	27.462824619592514	23.484399249824797
40	22.762880513887037	26.82358682483523	27.71846299201875	22.695069669258988
41	23.13569829564961	25.65973709820656	27.568371206210223	23.636193399933607
42	22.74245214228361	26.881467211044942	27.211442157916988	23.164638488754466
43	23.478440974773797	25.588521524977942	27.305923376582847	23.627114123665418
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	22.0
2	33.0
3	89.0
4	145.0
5	145.0
6	211.5
7	278.0
8	268.0
9	258.0
10	352.5
11	447.0
12	447.0
13	685.5
14	924.0
15	1554.0
16	2184.0
17	2353.5
18	2523.0
19	2523.0
20	2212.0
21	1901.0
22	2002.0
23	2103.0
24	2681.5
25	3260.0
26	3260.0
27	4205.5
28	5151.0
29	6544.0
30	7937.0
31	9802.5
32	11668.0
33	11668.0
34	13908.5
35	16149.0
36	18215.0
37	20281.0
38	22518.0
39	24755.0
40	24755.0
41	26326.0
42	27897.0
43	28952.0
44	30007.0
45	29581.0
46	29155.0
47	29155.0
48	28674.0
49	28193.0
50	28038.5
51	27884.0
52	26345.0
53	24806.0
54	24806.0
55	23552.0
56	22298.0
57	19582.0
58	16866.0
59	15480.5
60	14095.0
61	14095.0
62	12439.5
63	10784.0
64	8870.0
65	6956.0
66	5995.0
67	5034.0
68	5034.0
69	4164.5
70	3295.0
71	2723.5
72	2152.0
73	1729.5
74	1307.0
75	1307.0
76	1018.0
77	729.0
78	580.5
79	432.0
80	338.5
81	245.0
82	245.0
83	194.5
84	144.0
85	117.0
86	90.0
87	70.5
88	51.0
89	51.0
90	35.5
91	20.0
92	11.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	352451.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.37969856368041
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.5426342856181	53.11960805358073
2	6.956423502362438	8.539663553126168
3	2.2033463244775437	4.057221996834745
4	1.1333423907422246	2.782568572527946
5	0.6943784392679179	2.131036964569183
6	0.4588342051259751	1.6897863120802954
7	0.32528019825391724	1.397592036229175
8	0.23713060074382752	1.1644003835104466
9	0.18273128682515502	1.0094392173133298
>10	1.1004204955949195	12.633268477168787
>50	0.11094036195814864	4.71199262735765
>100	0.05313950110599848	5.808090979133696
>500	4.661359746140218E-4	0.17738997079127544
>1k	9.322719492280436E-4	0.7779408557765773
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1496	0.42445616553790455	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1223	0.34699858987490456	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	620	0.17591097769619038	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	417	0.11831431886985709	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	417	0.11831431886985709	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	394	0.11178858905209518	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	390	0.11065367951857137	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	357	0.10129067586699995	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.674547667619045E-4	0.0	0.0
2	0.0	0.0	8.511821501428567E-4	0.0	0.0
3	0.0	0.0	0.0014186369169047611	0.0	0.0
4	0.0	0.0	0.00255354645042857	0.0	0.0
5	0.0	0.0	0.003404728600571427	0.0	0.0
6	0.0	0.0	0.004823365517476188	0.0	0.0
7	0.0	0.0	0.009646731034952376	0.0	0.0
8	0.0	0.0	0.011349095335238089	0.0	0.0
9	0.0	0.0	0.013335187018904756	5.674547667619045E-4	0.0
10	0.001134909533523809	0.0	0.015321278702571422	0.001134909533523809	0.0
11	0.0014186369169047611	0.0	0.021847008520333323	0.001134909533523809	0.0
12	0.0014186369169047611	0.0	0.022698190670476178	0.001134909533523809	0.0
13	0.0014186369169047611	0.0	0.02411682758738094	0.001134909533523809	0.0
14	0.0014186369169047611	0.0	0.0255354645042857	0.001134909533523809	0.0
15	0.0014186369169047611	0.0	0.02638664665442856	0.001134909533523809	0.0
16	0.0014186369169047611	0.0	0.028656465721476176	0.001134909533523809	0.0
17	0.0014186369169047611	0.0	0.029223920488238082	0.001134909533523809	0.0
18	0.0014186369169047611	0.0	0.029791375254999985	0.0014186369169047611	0.0
19	0.0014186369169047611	0.0	0.03035883002176189	0.0014186369169047611	0.0
20	0.0014186369169047611	0.0	0.03177746693866665	0.0017023643002857134	0.0
21	0.0014186369169047611	0.0	0.03262864908880951	0.0019860916836666655	0.0
22	0.0014186369169047611	0.0	0.033479831238952366	0.0019860916836666655	0.0
23	0.0014186369169047611	0.0	0.033763558622333316	0.00255354645042857	0.0
24	0.0014186369169047611	0.0	0.033763558622333316	0.003688455983952379	0.0
25	0.0014186369169047611	0.0	0.033763558622333316	0.003972183367333331	0.0
26	0.0014186369169047611	0.0	0.033763558622333316	0.004539638134095236	0.0
27	0.0014186369169047611	0.0	0.03404728600571427	0.004823365517476188	0.0
28	0.0014186369169047611	2.8372738338095226E-4	0.03404728600571427	0.011349095335238089	0.0
29	0.0014186369169047611	2.8372738338095226E-4	0.03404728600571427	0.02894019310485713	0.0
30	0.0014186369169047611	2.8372738338095226E-4	0.03404728600571427	0.06468984341085711	0.0
31	0.0014186369169047611	2.8372738338095226E-4	0.03404728600571427	0.1378915083231428	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	750	0.0	22.693335	1
TTCGCCG	60	3.7168575E-5	21.583334	24
GGTATCA	345	0.0	21.449274	1
TGGGGCG	45	0.0038206265	20.555553	37
ACGGTAC	45	0.0038206265	20.555553	3
ACGGGTC	55	5.1333755E-4	20.181818	16
GCCGCTC	65	6.884789E-5	19.923077	27
GTCGCCC	85	1.2403016E-6	19.588236	37
GAGTTAG	120	5.136826E-9	18.5	26
CGGAGTT	120	5.136826E-9	18.5	24
TCACGGT	50	0.007025445	18.499998	1
CACGGTA	50	0.007025445	18.499998	2
CGCGGTA	50	0.007025445	18.499998	12
TTCCGAT	50	0.007025445	18.499998	16
CTTGGAC	50	0.007025445	18.499998	3
AATAGCG	145	1.5279511E-10	17.862068	6
CGTATTA	125	8.518327E-9	17.76	2
ATTACCG	125	8.518327E-9	17.76	5
GTTAGCC	125	8.518327E-9	17.76	28
AGTTAGC	125	8.518327E-9	17.76	27
>>END_MODULE