FastQCFastQC Report
Fri 10 Feb 2017
ERR1632885.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632885.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332314
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11370.3421462833344367No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8370.2518702191300998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4490.13511317609249085No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG3470.1044193142630163No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3400.10231287276491512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGACG200.001840053437.017
CTCGTAC250.00549079229.59999812
CGTACTG250.00549079229.59999814
AGCTTCG509.0657195E-625.89999821
GGTATCA2350.023.6170221
CGCTCTC551.8973866E-523.54545429
ATAAACC551.8973866E-523.5454543
CGAGTCG400.001928314323.12500221
TGAGCGG400.001928314323.12500230
GTATCAA6000.022.5083331
AGTCGCC502.6965048E-422.19999923
GGGGCGA502.6965048E-422.19999930
GCCGCTC603.716034E-521.58333427
CATAATA603.716034E-521.5833342
TTCGCCG705.0839335E-621.14285724
GTACTGG806.929513E-720.81251
GTATTAT450.003820173220.5555551
CCCTAGA450.003820173220.5555551
CGAGGGT1001.281478E-820.34999816
ACTAGCA555.132526E-420.1818188
Produced by FastQC (version 0.11.5)