##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632885.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	332314
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.90031716990557	33.0	33.0	33.0	27.0	33.0
2	30.612745776584795	33.0	33.0	33.0	27.0	33.0
3	31.04018187617735	33.0	33.0	33.0	27.0	33.0
4	31.227619059082674	33.0	33.0	33.0	27.0	33.0
5	31.331629121854633	33.0	33.0	33.0	27.0	33.0
6	33.86413452337247	37.0	33.0	37.0	27.0	37.0
7	34.10835835986447	37.0	33.0	37.0	27.0	37.0
8	34.29157965057145	37.0	37.0	37.0	27.0	37.0
9	34.373448003996224	37.0	37.0	37.0	27.0	37.0
10	34.3872090853831	37.0	37.0	37.0	27.0	37.0
11	34.39935121601859	37.0	37.0	37.0	27.0	37.0
12	34.39838827133374	37.0	37.0	37.0	27.0	37.0
13	34.43068001949963	37.0	37.0	37.0	27.0	37.0
14	34.39592975318524	37.0	37.0	37.0	27.0	37.0
15	34.39280319216163	37.0	37.0	37.0	27.0	37.0
16	34.41341622682162	37.0	37.0	37.0	27.0	37.0
17	34.39884567005904	37.0	37.0	37.0	27.0	37.0
18	34.40410274619787	37.0	37.0	37.0	27.0	37.0
19	34.40758439307402	37.0	37.0	37.0	27.0	37.0
20	34.405399712320275	37.0	37.0	37.0	27.0	37.0
21	34.406705706049095	37.0	37.0	37.0	27.0	37.0
22	34.40208658076398	37.0	37.0	37.0	27.0	37.0
23	34.38091684370806	37.0	37.0	37.0	27.0	37.0
24	34.362121367140716	37.0	37.0	37.0	27.0	37.0
25	34.36666526237234	37.0	37.0	37.0	27.0	37.0
26	34.31474147944414	37.0	37.0	37.0	27.0	37.0
27	34.23096529186252	37.0	37.0	37.0	27.0	37.0
28	34.201396871633456	37.0	37.0	37.0	27.0	37.0
29	34.25585440276365	37.0	37.0	37.0	27.0	37.0
30	34.18672400199811	37.0	37.0	37.0	27.0	37.0
31	34.174076325403085	37.0	37.0	37.0	27.0	37.0
32	34.21503758493473	37.0	37.0	37.0	27.0	37.0
33	34.16720631691713	37.0	37.0	37.0	27.0	37.0
34	34.0875376902568	37.0	37.0	37.0	27.0	37.0
35	34.08652659833772	37.0	37.0	37.0	27.0	37.0
36	34.0615261469574	37.0	33.0	37.0	27.0	37.0
37	34.093754701878346	37.0	37.0	37.0	27.0	37.0
38	33.96231576159897	37.0	33.0	37.0	27.0	37.0
39	34.040886029478145	37.0	33.0	37.0	27.0	37.0
40	34.058640322105	37.0	33.0	37.0	27.0	37.0
41	34.09556624156671	37.0	37.0	37.0	27.0	37.0
42	33.97023297242969	37.0	33.0	37.0	27.0	37.0
43	33.720135173360134	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	12.0
16	30.0
17	32.0
18	20.0
19	31.0
20	88.0
21	259.0
22	555.0
23	1195.0
24	2282.0
25	3742.0
26	5465.0
27	7315.0
28	9658.0
29	12234.0
30	14748.0
31	18074.0
32	21813.0
33	27439.0
34	37494.0
35	61791.0
36	108036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.579993620491464	20.981962842371974	13.06746029357776	25.3705832435588
2	16.578597350698434	23.555733432837616	36.25035358125147	23.61531563521248
3	18.16565055941068	27.267283352491923	30.05561005554987	24.511456032547528
4	13.486040311271868	18.494255433114464	38.50454690443376	29.51515735117991
5	14.647893257581684	37.11309183483091	34.95820218227339	13.280812725314009
6	29.71857941585368	39.95678785726752	17.165391768026623	13.15924095885217
7	26.609170844442303	32.282118719042835	23.573788645678484	17.53492179083638
8	24.77415937938215	35.841703930619836	20.568197548102095	18.815939141895917
9	26.182165060755793	15.016219599535379	20.096655572741444	38.704959766967384
10	15.01290947718122	28.058402595135927	34.192661157820616	22.73602676986224
11	34.10900533832459	23.615014714998463	24.02185884434601	18.254121102330927
12	22.91025957377661	25.759673080279494	30.15070084317844	21.179366502765458
13	27.854378690034125	22.18173173564761	26.014251581335724	23.949637992982538
14	22.05203512340738	20.644330362247754	27.656373189212612	29.647261325132256
15	23.78894659869882	28.65723382102469	24.511456032547528	23.042363547728957
16	22.81577062657607	28.15198878169442	25.36516667970654	23.667073912022964
17	22.5187623753438	27.384341315743544	26.472252147065728	23.624644161846927
18	22.818779828716217	27.27390359720024	27.611536077324455	22.29578049675909
19	24.552381181653494	26.290496337800995	27.3271664750808	21.829956005464712
20	23.96979964732151	26.43584080116998	27.287144086616877	22.307215464891637
21	23.191319053666113	27.21733059696552	27.067171410172307	22.524178939196062
22	23.12180648422877	26.82824076024483	27.002473564159196	23.047479191367202
23	23.023706494460058	26.78912113242295	27.617554481604746	22.569617891512245
24	23.233447883628138	27.109601160348344	26.35639786467016	23.30055309135336
25	23.28099327744242	26.702456110786787	27.514639768411804	22.501910843358992
26	23.23134144213003	27.173095325505397	27.21522415546742	22.38033907689715
27	23.38691719277551	26.98983491517059	26.53845459414891	23.084793297904994
28	22.61926972682463	26.71359015870532	27.870628381590905	22.796511732879143
29	23.89246315231979	26.546278519713283	26.99043675559862	22.570821572368303
30	23.340274559603266	26.56824569533634	27.39126248066588	22.700217264394517
31	23.01377612739758	26.681993536233804	27.46107597031723	22.843154366051387
32	22.454967289972736	26.595027594383623	27.454455725608913	23.495549390034725
33	22.557581082951668	26.747594142888953	27.703015822384852	22.991808951774527
34	23.21268438886114	26.443664726734355	27.778546796102482	22.565104088302025
35	23.106459553314036	27.01661681421788	27.29436617175322	22.582557460714867
36	23.51330368266158	26.94499780328244	26.681692616019788	22.860005898036196
37	22.855191174611964	26.478270551346018	27.179113729785687	23.487424544256335
38	23.268053708239798	26.725326047051883	26.98893215452855	23.01768809017977
39	22.654778312078335	26.711182796993207	27.491769832146705	23.142269058781757
40	22.689083216475982	27.00728828758343	27.685261529757998	22.618366966182588
41	22.84435804690744	26.25859879511546	27.517949890765962	23.379093267211132
42	22.59760347141559	26.99916344180504	27.40660941158061	22.99662367519876
43	23.18259236745969	25.815042399658157	27.593179944269576	23.409185288612576
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	13.5
2	15.0
3	61.5
4	108.0
5	108.0
6	156.5
7	205.0
8	197.0
9	189.0
10	259.5
11	330.0
12	330.0
13	502.0
14	674.0
15	1135.0
16	1596.0
17	1797.5
18	1999.0
19	1999.0
20	1849.5
21	1700.0
22	1951.0
23	2202.0
24	2984.0
25	3766.0
26	3766.0
27	4942.5
28	6119.0
29	7494.0
30	8869.0
31	10918.5
32	12968.0
33	12968.0
34	15283.0
35	17598.0
36	19446.5
37	21295.0
38	22980.5
39	24666.0
40	24666.0
41	25753.5
42	26841.0
43	27427.5
44	28014.0
45	27654.0
46	27294.0
47	27294.0
48	26007.0
49	24720.0
50	24552.0
51	24384.0
52	23046.5
53	21709.0
54	21709.0
55	20848.5
56	19988.0
57	17377.0
58	14766.0
59	13369.0
60	11972.0
61	11972.0
62	10665.0
63	9358.0
64	7592.0
65	5826.0
66	4966.5
67	4107.0
68	4107.0
69	3461.0
70	2815.0
71	2427.0
72	2039.0
73	1923.0
74	1807.0
75	1807.0
76	1555.0
77	1303.0
78	952.5
79	602.0
80	405.0
81	208.0
82	208.0
83	155.0
84	102.0
85	95.0
86	88.0
87	61.5
88	35.0
89	35.0
90	27.0
91	19.0
92	11.0
93	3.0
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	332314.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.4981417762754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.90056085444553	59.78220241465594
2	5.419424386632798	7.207633024162244
3	1.5181375212063097	3.0285997236318143
4	0.7260074473006614	1.9311258464492467
5	0.45588770270269	1.5157842544191986
6	0.3191697655634491	1.273451779268329
7	0.20959240468104595	0.9756253809197476
8	0.17854514564454863	0.9498336326829547
9	0.15554601351389258	0.9309168773443152
>10	0.9727686496417833	12.655247743575096
>50	0.10565156217923688	4.780155602169364
>100	0.037797757158947454	4.371639295084688
>500	4.5539466456563193E-4	0.2534678643658828
>1k	4.5539466456563193E-4	0.3443165612712171
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1137	0.3421462833344367	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	837	0.2518702191300998	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	449	0.13511317609249085	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	347	0.1044193142630163	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	340	0.10231287276491512	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.009202140144562E-4	0.0	0.0
2	6.018404280289124E-4	0.0	3.009202140144562E-4	0.0	0.0
3	6.018404280289124E-4	0.0	3.009202140144562E-4	0.0	0.0
4	6.018404280289124E-4	0.0	6.018404280289124E-4	3.009202140144562E-4	0.0
5	6.018404280289124E-4	0.0	0.001504601070072281	3.009202140144562E-4	0.0
6	6.018404280289124E-4	0.0	0.0018055212840867372	3.009202140144562E-4	0.0
7	6.018404280289124E-4	0.0	0.005717484066274668	3.009202140144562E-4	0.0
8	6.018404280289124E-4	0.0	0.006921164922332493	6.018404280289124E-4	0.0
9	6.018404280289124E-4	0.0	0.008124845778390318	0.001504601070072281	0.0
10	6.018404280289124E-4	0.0	0.009328526634448142	0.0018055212840867372	0.0
11	9.027606420433686E-4	0.0	0.01504601070072281	0.0018055212840867372	0.0
12	9.027606420433686E-4	0.0	0.01594877134276618	0.0021064414981011932	0.0
13	9.027606420433686E-4	0.0	0.018657053268896284	0.0021064414981011932	0.0
14	9.027606420433686E-4	0.0	0.019258893696925197	0.0021064414981011932	0.0
15	9.027606420433686E-4	0.0	0.021967175623055304	0.0021064414981011932	0.0
16	0.0012036808560578248	0.0	0.023471776693127583	0.0024073617121156497	0.0
17	0.0018055212840867372	0.0	0.02377269690714204	0.0024073617121156497	0.0
18	0.0018055212840867372	0.0	0.024073617121156497	0.0024073617121156497	0.0
19	0.0018055212840867372	0.0	0.02467545754918541	0.0024073617121156497	0.0
20	0.0018055212840867372	0.0	0.02467545754918541	0.0024073617121156497	0.0
21	0.0018055212840867372	0.0	0.02618005861925769	0.0027082819261301056	0.0
22	0.0018055212840867372	0.0	0.026480978833272144	0.003009202140144562	0.0
23	0.0018055212840867372	0.0	0.027082819261301058	0.0036110425681734745	0.0
24	0.0018055212840867372	0.0	0.027383739475315513	0.0042128829962023865	0.0
25	0.0018055212840867372	0.0	0.027383739475315513	0.0042128829962023865	0.0
26	0.0018055212840867372	0.0	0.027383739475315513	0.004513803210216843	0.0
27	0.0018055212840867372	0.0	0.027383739475315513	0.005717484066274668	0.0
28	0.0018055212840867372	0.0	0.027383739475315513	0.014444170272693898	0.0
29	0.0018055212840867372	0.0	0.027383739475315513	0.0315966224715179	0.0
30	0.0018055212840867372	0.0	0.027383739475315513	0.059883122588876785	0.0
31	0.0018055212840867372	0.0	0.027383739475315513	0.127289250528115	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGACG	20	0.0018400534	37.0	17
CTCGTAC	25	0.005490792	29.599998	12
CGTACTG	25	0.005490792	29.599998	14
AGCTTCG	50	9.0657195E-6	25.899998	21
GGTATCA	235	0.0	23.617022	1
CGCTCTC	55	1.8973866E-5	23.545454	29
ATAAACC	55	1.8973866E-5	23.545454	3
CGAGTCG	40	0.0019283143	23.125002	21
TGAGCGG	40	0.0019283143	23.125002	30
GTATCAA	600	0.0	22.508333	1
AGTCGCC	50	2.6965048E-4	22.199999	23
GGGGCGA	50	2.6965048E-4	22.199999	30
GCCGCTC	60	3.716034E-5	21.583334	27
CATAATA	60	3.716034E-5	21.583334	2
TTCGCCG	70	5.0839335E-6	21.142857	24
GTACTGG	80	6.929513E-7	20.8125	1
GTATTAT	45	0.0038201732	20.555555	1
CCCTAGA	45	0.0038201732	20.555555	1
CGAGGGT	100	1.281478E-8	20.349998	16
ACTAGCA	55	5.132526E-4	20.181818	8
>>END_MODULE