FastQCFastQC Report
Fri 10 Feb 2017
ERR1632884.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632884.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433451
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35030.8081651674583747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27170.6268297916027417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13790.318144380795061No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC13020.3003799737455906No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12390.28584545888693297No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG11590.2673889320822884No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA11380.26254409379606924No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG11320.2611598542857209No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG10910.25170088429834053No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC10710.24708675259717938No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG10500.24224191431096018No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10200.23532071675921845No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT10140.2339364772488701No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT9600.22147832165573503No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG9490.21894054922009637No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA9480.21870984263503832No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG8980.2071745133821355No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA8610.19863836973498736No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG8420.19425494461888426No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT8360.1928707051085359No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC8320.1919478787683037No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC8150.1880258668223167No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT7910.18248890878092333No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7810.18018184293034276No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG7500.173029938793543No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA7390.17049216635790435No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC7270.16772368733720766No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA7100.1638016753912207No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT6960.16057178320040788No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6940.16011037003029177No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA6890.1589568371050015No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG6870.15849542393488536No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG6840.1578033041797112No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT6750.1557269449141887No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT6750.1557269449141887No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT6690.15434270540384035No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC6680.1541119988187823No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT6610.15249705272337588No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG6320.1458065617566922No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC6280.14488373541646No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC6200.14303808273599553No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG6090.1405003103003569No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5920.13657829835436994No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG5880.1356554720141377No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT5730.13219487323826684No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC5700.13150275348309265No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA5580.128734274462396No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC5570.1285035678773379No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5520.12735003495204764No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT5520.12735003495204764No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT5350.12342802300606065No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT5320.12273590325088649No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5320.12273590325088649No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG5240.12089025057042202No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC5210.12019813081524786No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG5200.11996742423018981No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT5150.11881389130489951No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA5110.11789106496466728No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA5110.11789106496466728No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC5080.11719894520949312No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA5010.11558399911408672No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC4990.1151225859439706No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA4830.11143128058304168No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG4790.11050845424280945No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT4790.11050845424280945No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG4750.10958562790257723No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG4740.10935492131751916No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT4730.10912421473246112No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT4710.108662801562345No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG4710.108662801562345No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG4690.10820138839222888No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4630.10681714888188054No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA4600.10612502912670635No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGG4550.10497149620141608No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGC4530.10451008303129995No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTAC4440.10243372376577745No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT4420.10197231059566132No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG4420.10197231059566132No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA4370.10081877767037105No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCC4360.10058807108531298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATCT303.5958135E-430.83333234
TTGTACT250.00549261429.5999984
GGAACCC250.00549261429.59999816
GGACCCA250.00549261429.59999834
TGTACTA250.00549261429.5999985
GGTATCA6100.027.9016381
ATCTCGT358.860446E-426.42857237
CGAGTCT358.860446E-426.42857234
CATCTCG358.860446E-426.42857236
ATAGATC358.860446E-426.4285726
CCCACGA358.860446E-426.42857219
GAGACGG358.860446E-426.42857224
GCAGGGC358.860446E-426.42857229
CGAGCCC358.860446E-426.42857215
TAGATCT358.860446E-426.4285727
GACATCT358.860446E-426.42857218
GATCTTG358.860446E-426.4285729
CCGCCGC951.0913936E-1125.3157932
GCCGTCC1100.025.22727227
GGTGTGT451.3213552E-424.66666636
Produced by FastQC (version 0.11.5)