FastQCFastQC Report
Fri 10 Feb 2017
ERR1632883.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632883.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480209
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42530.8856560372671065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32340.6734567656999348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16540.3444333613072641No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA13450.2800863790557861No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC12380.25780441432792806No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG12100.2519736198197035No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11570.2409367587862785No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11440.23822960419317424No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC11380.2369801482271261No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG11030.22969165509184541No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC10830.22552680187168506No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10370.21594763946531614No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG10310.214698183499268No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT10250.2134487275332199No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG9930.2067849623809633No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT9900.20616023439793923No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC9730.2026201091608029No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9420.19616458666955433No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT9100.1895008215172977No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG9090.18929257885628967No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8880.18491948297512126No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA8520.17742274717883255No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8460.17617329121278444No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8230.17138371000959998No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC8190.1705507393655679No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA7770.1618045476032311No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7500.15618199575601457No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC7440.15493253978996643No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT7350.15305835584089428No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA7320.15243362785787024No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT7220.15035120124779003No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7190.14972647326476596No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7150.1488935026207339No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG7100.14785228931569377No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG6950.1447286494005735No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT6940.14452040673956548No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6910.14389567875654144No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6890.1434791934345254No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC6890.1434791934345254No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG6880.14327095077351737No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC6420.13369178836714846No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6360.13244233240110034No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC6360.13244233240110034No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6210.12931869248598007No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG6200.12911044982497205No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT6190.12890220716396403No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG6080.12661153789287583No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA5950.12390438329977155No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA5780.12036425806263523No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG5740.11953128741860315No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5720.11911480209658712No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5710.1189065594355791No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5690.11849007411356305No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5490.11432522089340268No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT5460.11370049291037862No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5440.11328400758836257No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC5430.11307576492735455No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5420.11286752226634654No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG5400.11245103694433049No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA5310.11057685299525831No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5210.10849442638517812No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5200.1082861837241701No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG5180.10786969840215406No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5170.10766145574114604No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC5170.10766145574114604No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5160.10745321308013801No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG5130.10682848509711396No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5130.10682848509711396No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5110.10641199977509794No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT5050.10516254380904981No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC5020.10453781582602575No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT5010.10432957316501773No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA4890.10183066123292149No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4860.10120593324989743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAA200.001840869337.022
ACTAGTG352.3854334E-531.7142878
TAACGCC250.005493195729.619
TAGCCTC250.005493195729.65
CGTAAAT250.005493195729.615
GCGTGAG250.005493195729.69
GGTATCA8650.028.017341
GTATCAA22600.023.1659281
GCCGTCC1900.022.39473727
TTGCGGT502.6989283E-422.235
TTGGATG450.003822613320.5555553
TCGGAAC450.003822613320.55555522
TGCTCGC2250.020.55555510
GCGGTAA1001.285116E-820.3523
ATATAGC555.1371014E-420.1818186
CATAATA555.1371014E-420.1818182
CGCCGCC1850.020.033
TCCGCCG1950.019.92307531
CGCCGTC2050.019.8536626
CAAAAAC1052.2511813E-819.3809521
Produced by FastQC (version 0.11.5)