FastQCFastQC Report
Fri 10 Feb 2017
ERR1632882.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632882.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326643
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6820.20879063687267138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5070.15521532682469852No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3740.11449809118823916No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3470.10623218620940905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCA358.855749E-426.42857215
CGCCGGC509.065094E-625.90000214
GTATCAA4150.024.0722891
CGCTCTC400.001928244523.12529
GGTATCA1700.022.8529421
TTAACGG1155.456968E-1222.5217435
CTGCTCG1650.022.4242429
TAACGGC1251.8189894E-1120.7236
AACGGCC1251.8189894E-1120.7237
CTGCGTT450.003820033520.55555735
TAAGGGT450.003820033520.5555574
GAATTAG450.003820033520.5555571
TTTATAC555.132268E-420.1818183
AATAGTC555.132268E-420.18181825
AGGTCGC1101.7407729E-920.18181835
ACTGCTC1950.019.9230778
TGCTCGC656.882812E-519.92307710
ATTAGAG1600.019.656253
GCTCGGA1153.0267984E-919.30434811
GTATTAG2100.018.51
Produced by FastQC (version 0.11.5)