##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632882.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	326643
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.232890342055395	33.0	33.0	33.0	27.0	33.0
2	30.877722773792794	33.0	33.0	33.0	27.0	33.0
3	31.3101918608389	33.0	33.0	33.0	27.0	33.0
4	31.464017291048638	33.0	33.0	33.0	27.0	33.0
5	31.58859978631105	33.0	33.0	33.0	27.0	33.0
6	34.30808558579244	37.0	33.0	37.0	27.0	37.0
7	34.52819745103982	37.0	37.0	37.0	27.0	37.0
8	34.692713451688846	37.0	37.0	37.0	27.0	37.0
9	34.79001233762854	37.0	37.0	37.0	27.0	37.0
10	34.77393056027528	37.0	37.0	37.0	27.0	37.0
11	34.82234427188092	37.0	37.0	37.0	27.0	37.0
12	34.793695257513555	37.0	37.0	37.0	27.0	37.0
13	34.826281291807874	37.0	37.0	37.0	27.0	37.0
14	34.79741491475403	37.0	37.0	37.0	27.0	37.0
15	34.813380357148326	37.0	37.0	37.0	27.0	37.0
16	34.80857082502916	37.0	37.0	37.0	27.0	37.0
17	34.77094565014404	37.0	37.0	37.0	27.0	37.0
18	34.77877070685734	37.0	37.0	37.0	27.0	37.0
19	34.77179979365852	37.0	37.0	37.0	27.0	37.0
20	34.76986495960421	37.0	37.0	37.0	27.0	37.0
21	34.79673833512428	37.0	37.0	37.0	27.0	37.0
22	34.79666486041336	37.0	37.0	37.0	27.0	37.0
23	34.78179847723661	37.0	37.0	37.0	27.0	37.0
24	34.76680351331576	37.0	37.0	37.0	27.0	37.0
25	34.769549630636504	37.0	37.0	37.0	27.0	37.0
26	34.70239374485294	37.0	37.0	37.0	27.0	37.0
27	34.667578977660625	37.0	37.0	37.0	27.0	37.0
28	34.61999797944545	37.0	37.0	37.0	27.0	37.0
29	34.6438252159085	37.0	37.0	37.0	27.0	37.0
30	34.6075991219772	37.0	37.0	37.0	27.0	37.0
31	34.59604522368457	37.0	37.0	37.0	27.0	37.0
32	34.623019626932155	37.0	37.0	37.0	27.0	37.0
33	34.622508365401984	37.0	37.0	37.0	27.0	37.0
34	34.53875025639613	37.0	37.0	37.0	27.0	37.0
35	34.53531225221419	37.0	37.0	37.0	27.0	37.0
36	34.507829648882726	37.0	37.0	37.0	27.0	37.0
37	34.552309402007694	37.0	37.0	37.0	27.0	37.0
38	34.397712487333266	37.0	37.0	37.0	27.0	37.0
39	34.486650563459186	37.0	37.0	37.0	27.0	37.0
40	34.527771910005725	37.0	37.0	37.0	27.0	37.0
41	34.52032341118591	37.0	37.0	37.0	27.0	37.0
42	34.4305862975787	37.0	37.0	37.0	27.0	37.0
43	34.18547160049351	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	18.0
16	24.0
17	24.0
18	23.0
19	28.0
20	66.0
21	172.0
22	381.0
23	818.0
24	1492.0
25	2555.0
26	4092.0
27	5877.0
28	7779.0
29	10147.0
30	12594.0
31	15638.0
32	19142.0
33	24329.0
34	33714.0
35	57922.0
36	129807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.143826746631646	21.28011315105482	13.26524676787808	25.310813334435455
2	16.2697501553684	23.368631809039226	37.117587090493295	23.244030945099084
3	17.753020882125135	27.040530487412862	30.328829945843015	24.877618684618987
4	12.987573589515158	18.37388218942393	38.93241245028977	29.70613177077115
5	14.037343521826582	37.26514880159685	35.661257091074965	13.036250585501602
6	29.682252489721193	40.1845439822681	17.407995885416188	12.725207642594514
7	26.36456314692187	32.399592215354375	23.911426235982404	17.32441840174135
8	24.060824814859036	36.62163279176349	20.864062600453707	18.453479792923773
9	25.63777579804251	15.471018818710336	20.444950603564134	38.44625477968302
10	14.43869913024311	28.646871354965516	34.68465572505763	22.229773789733745
11	34.0763463475415	23.629773177444488	24.3287013650989	17.965179109915105
12	22.48387383167556	25.900141744963157	30.861521600034287	20.754462823326996
13	27.612714798725214	22.3846829719296	26.43069038675251	23.571911842592677
14	21.550745002954297	21.02723768762839	27.697210716286584	29.724806593130726
15	23.621201127836812	29.263446637460472	24.223693757404874	22.891658477297845
16	22.942784630315053	28.81433246694403	25.32612056587773	22.91676233686318
17	22.092008706753244	28.014682696399433	26.825616957963284	23.067691638884043
18	22.51356986067358	27.898347737438122	27.92161472923038	21.666467672657916
19	24.0599063809725	27.07298181807049	27.207379310133696	21.659732490823313
20	23.687328367667455	27.149824119910726	27.379126446916054	21.783721065505766
21	22.82644967135374	27.466989955394727	27.47586814963125	22.23069222362028
22	22.55275637316581	27.35402258735072	27.275343417737407	22.817877621746067
23	22.665417596580976	27.175234124104907	27.8202808570825	22.339067422231608
24	22.812979307684536	28.07131945273586	26.17965179109915	22.93604944848045
25	23.002176688311092	27.274118839222027	27.543526112606116	22.180178359860765
26	22.85216582017677	27.607510340034842	27.319428244291167	22.220895595497225
27	22.80655027047878	27.56342551348108	26.75887742887495	22.87114678716519
28	22.343659591664295	27.309325471539264	27.8068104934133	22.540204443383143
29	23.627936309671416	27.26217919869705	26.860517445651677	22.249367045979863
30	23.159535027537707	27.07604326435895	27.302590289704664	22.46183141839868
31	23.04228163468986	27.05614386348399	27.39045379818334	22.511120703642813
32	22.24477487654718	26.964912764088012	27.607510340034842	23.18280201932997
33	22.200077760735727	27.489032368671605	27.814464109134434	22.496425761458227
34	22.998809097393792	26.938278181378443	27.67976047244239	22.383152248785372
35	22.84451220445563	27.401168860192932	27.430864889190953	22.32345404616049
36	23.610792210456065	27.19360280183564	26.78030755289414	22.415297434814153
37	22.702767241300133	26.806635990974858	27.410047054429455	23.080549713295557
38	22.932375712934306	27.259730041666288	27.13176158680884	22.67613265859057
39	22.60388252618302	27.170335810043383	27.47066369094087	22.755117972832725
40	22.520917331765872	27.584549492871425	27.635981790517476	22.25855138484523
41	22.586738426967667	26.672238498911653	27.654044323619363	23.08697875050131
42	22.432441534029508	27.39106608744103	27.509237914175415	22.667254464354052
43	22.94217234105736	26.37680893207569	27.73700951803651	22.944009208830433
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	10.0
2	13.0
3	37.5
4	62.0
5	62.0
6	96.0
7	130.0
8	128.0
9	126.0
10	161.0
11	196.0
12	196.0
13	296.0
14	396.0
15	730.5
16	1065.0
17	1265.5
18	1466.0
19	1466.0
20	1581.0
21	1696.0
22	2151.0
23	2606.0
24	3509.0
25	4412.0
26	4412.0
27	5697.0
28	6982.0
29	8663.0
30	10344.0
31	12314.0
32	14284.0
33	14284.0
34	16360.0
35	18436.0
36	20166.0
37	21896.0
38	23636.5
39	25377.0
40	25377.0
41	26112.5
42	26848.0
43	27520.0
44	28192.0
45	27444.0
46	26696.0
47	26696.0
48	25261.0
49	23826.0
50	23543.0
51	23260.0
52	21944.5
53	20629.0
54	20629.0
55	19367.5
56	18106.0
57	15811.5
58	13517.0
59	12435.5
60	11354.0
61	11354.0
62	10064.0
63	8774.0
64	7224.0
65	5674.0
66	4748.5
67	3823.0
68	3823.0
69	3201.0
70	2579.0
71	2079.5
72	1580.0
73	1264.0
74	948.0
75	948.0
76	741.0
77	534.0
78	424.0
79	314.0
80	253.5
81	193.0
82	193.0
83	162.0
84	131.0
85	122.5
86	114.0
87	72.5
88	31.0
89	31.0
90	25.5
91	20.0
92	12.0
93	4.0
94	3.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	326643.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.86876692131082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.89068798128443	62.80551526960638
2	5.683993759478268	7.9426727032634465
3	1.5621009636678447	3.2742620441419104
4	0.7321466593404048	2.046167371746844
5	0.465016293457582	1.6245057511099836
6	0.28256322914314225	1.1845406638521085
7	0.20406165038719348	0.9980275121936586
8	0.14724876944469095	0.8230471961424757
9	0.11384335798155004	0.7158685539917042
>10	0.8086368018885485	10.874887178455927
>50	0.07709876882375012	3.6057672729405166
>100	0.031720636316046616	3.7387441776612675
>500	8.811287865568506E-4	0.36599430489373025
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	682	0.20879063687267138	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	507	0.15521532682469852	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	374	0.11449809118823916	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	347	0.10623218620940905	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.0614462884555923E-4	0.0
5	0.0	0.0	0.0	3.0614462884555923E-4	0.0
6	0.0	0.0	3.0614462884555923E-4	3.0614462884555923E-4	0.0
7	0.0	0.0	6.122892576911185E-4	3.0614462884555923E-4	0.0
8	0.0	0.0	9.184338865366777E-4	3.0614462884555923E-4	0.0
9	0.0	0.0	9.184338865366777E-4	3.0614462884555923E-4	0.0
10	0.0	0.0	0.001530723144227796	3.0614462884555923E-4	0.0
11	0.0	0.0	0.0021430124019189145	6.122892576911185E-4	0.0
12	0.0	0.0	0.0021430124019189145	6.122892576911185E-4	0.0
13	0.0	0.0	0.002449157030764474	6.122892576911185E-4	0.0
14	0.0	0.0	0.002449157030764474	6.122892576911185E-4	0.0
15	0.0	0.0	0.0027553016596100328	6.122892576911185E-4	0.0
16	0.0	0.0	0.0033675909173011514	6.122892576911185E-4	0.0
17	0.0	0.0	0.0036737355461467108	6.122892576911185E-4	0.0
18	0.0	0.0	0.0036737355461467108	6.122892576911185E-4	0.0
19	0.0	0.0	0.0036737355461467108	6.122892576911185E-4	0.0
20	0.0	0.0	0.0036737355461467108	6.122892576911185E-4	0.0
21	0.0	0.0	0.00397988017499227	6.122892576911185E-4	0.0
22	0.0	0.0	0.00397988017499227	9.184338865366777E-4	0.0
23	0.0	0.0	0.00397988017499227	9.184338865366777E-4	0.0
24	0.0	0.0	0.00397988017499227	9.184338865366777E-4	0.0
25	0.0	0.0	0.00397988017499227	9.184338865366777E-4	0.0
26	0.0	0.0	0.00397988017499227	0.001224578515382237	0.0
27	0.0	0.0	0.00397988017499227	0.003061446288455592	0.0
28	0.0	0.0	0.00397988017499227	0.008878194236521217	0.0
29	0.0	0.0	0.00397988017499227	0.01959325624611579	0.0
30	0.0	0.0	0.00397988017499227	0.03490048768839375	0.0
31	0.0	0.0	0.00397988017499227	0.08725121922098437	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGGCA	35	8.855749E-4	26.428572	15
CGCCGGC	50	9.065094E-6	25.900002	14
GTATCAA	415	0.0	24.072289	1
CGCTCTC	40	0.0019282445	23.125	29
GGTATCA	170	0.0	22.852942	1
TTAACGG	115	5.456968E-12	22.52174	35
CTGCTCG	165	0.0	22.424242	9
TAACGGC	125	1.8189894E-11	20.72	36
AACGGCC	125	1.8189894E-11	20.72	37
CTGCGTT	45	0.0038200335	20.555557	35
TAAGGGT	45	0.0038200335	20.555557	4
GAATTAG	45	0.0038200335	20.555557	1
TTTATAC	55	5.132268E-4	20.181818	3
AATAGTC	55	5.132268E-4	20.181818	25
AGGTCGC	110	1.7407729E-9	20.181818	35
ACTGCTC	195	0.0	19.923077	8
TGCTCGC	65	6.882812E-5	19.923077	10
ATTAGAG	160	0.0	19.65625	3
GCTCGGA	115	3.0267984E-9	19.304348	11
GTATTAG	210	0.0	18.5	1
>>END_MODULE