FastQCFastQC Report
Fri 10 Feb 2017
ERR1632881.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632881.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492145
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19690.4000853407024353No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC19440.39500553698605084No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG17850.3626979853498461No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC17090.3472553820520375No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG16130.3277489357811214No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA15610.31718294405104186No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT15530.31555740686179884No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC15450.3139318696725559No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC14970.3041786465370978No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14780.30031799571264567No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT14420.2930030783610521No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG14280.2901583882798769No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG14160.28772008249601233No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG13320.27065194200896076No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG12030.24444015483241727No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA11700.23773481392678986No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA11580.23529650814292533No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC11430.2322486259130947No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT11360.2308262808725071No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG11260.22879435938595333No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC11100.2255432850074673No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT10790.21924432839915067No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC10780.2190411362504953No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG10440.2121326031962125No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG10340.21010068170965873No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT9380.19059423543874265No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA9280.1885623139521889No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT9150.18592081601966898No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA8890.18063782015462923No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG8710.17698036147883245No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT8670.17616759288421094No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA8620.17515163214093407No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT8620.17515163214093407No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA8590.17454205569496795No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT8530.1733229028030357No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC8290.16844629123530666No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC8130.16519521685682065No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC8060.163772871816233No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC7950.16153775818102387No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT7880.16011541314043626No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC7730.15706753091060563No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG7610.1546292251267411No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7460.15158134289691044No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG7390.15015899785632283No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT7290.14812707636976907No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG7210.14650153918052605No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT7170.14568877058590454No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG7070.1436568490993508No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC7000.14223450405876317No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG6980.1418281197614524No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT6920.14060896686952015No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT6890.13999939042355403No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG6800.13817066108565565No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT6760.13735789249103417No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA6700.1361387395991019No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTAC6630.13471639455851425No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC6590.13390362596389277No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG6580.13370043381523739No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT6540.13288766522061587No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT6540.13288766522061587No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG6530.1326844730719605No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA6410.130246167288096No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGG6390.12983978299078522No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA6380.12963659084212986No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC6370.12943339869347448No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT6350.12902701439616374No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA6300.12801105365288684No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA6130.12455678712574546No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGC5970.12130571274725943No Hit
CCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT5910.12008655985532718No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCC5810.11805463836877343No Hit
ACCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGA5800.11785144622011806No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA5760.11703867762549655No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA5720.11622590903087504No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAG5620.1141939875443213No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATT5470.11114610531449065No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG5460.11094291316583527No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAAT5440.11053652886852453No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGC5280.10728545449003851No Hit
AAACAGTACCTAACAAACCCACAGGTCCTAAACTACCAAACCT5260.10687907019272776No Hit
GATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGAT5220.10606630159810625No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCG5170.10505034085482937No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT5140.10444076440886325No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTAC5110.10383118796289711No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAAT5080.10322161151693099No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCT5050.10261203507096485No Hit
CTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGA4970.10098649788172186No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGGG200.001840913737.03
CGTTTAT200.001840913737.033
GTAGTGT200.001840913737.015
TCGTCCC251.2326002E-436.99999630
AATCGTC251.2326002E-436.99999628
ATCGTCC303.5965143E-430.83333229
TGCTAAA303.5965143E-430.83333218
CGTCCCA303.5965143E-430.83333231
TCCCAAC303.5965143E-430.83333233
CTAATCG303.5965143E-430.83333226
GGTATCA4000.030.5249981
AAACTTT250.00549332829.59999826
TACTTTG250.00549332829.59999828
ATTAGAC250.00549332829.5999983
TTAGACT250.00549332829.5999984
CTGGTCA250.00549332829.59999831
CAAGGCC250.00549332829.5999984
TAGATTA250.00549332829.59999835
AATACTT250.00549332829.59999826
TGGTCAT250.00549332829.59999832
Produced by FastQC (version 0.11.5)