FastQCFastQC Report
Fri 10 Feb 2017
ERR1632880.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632880.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346065
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10110.2921416496900871No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7370.21296577232600813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3960.1144293702050193No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGCC250.00549110329.59999827
CGCGGCG250.00549110329.59999827
GCCGGCA250.00549110329.59999815
TGGTCGA405.9283222E-527.7510
GGTCGAT358.8568195E-426.42857211
CCTACCG400.001928475823.12524
CGATTGG400.001928475823.12514
TCGATAC400.001928475823.1251
GTATCAA5500.022.8727261
GGTATCA2050.022.5609761
GTCGATT450.00382048820.55555512
CGTGCAA450.00382048820.55555511
AGACGAT450.00382048820.55555529
CTTATGG450.00382048820.5555551
CTACCGA450.00382048820.55555525
GTATCAG450.00382048820.5555551
CGGAGTC555.1331177E-420.18181827
CTCTAGT1202.3646862E-1020.04166627
TGAACTT1303.274181E-1119.92307737
ACACTTA656.884326E-519.9230776
Produced by FastQC (version 0.11.5)