##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632880.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346065
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.520474477338073	33.0	33.0	33.0	27.0	33.0
2	31.07807492812044	33.0	33.0	33.0	27.0	33.0
3	31.523404562726654	33.0	33.0	33.0	27.0	33.0
4	31.675292213890454	33.0	33.0	33.0	27.0	33.0
5	31.794581942698628	33.0	33.0	33.0	33.0	33.0
6	34.65063788594628	37.0	37.0	37.0	27.0	37.0
7	34.838102668573825	37.0	37.0	37.0	27.0	37.0
8	35.005935301171746	37.0	37.0	37.0	33.0	37.0
9	35.095435828529325	37.0	37.0	37.0	33.0	37.0
10	35.127308453614205	37.0	37.0	37.0	33.0	37.0
11	35.16209382630431	37.0	37.0	37.0	33.0	37.0
12	35.10168898906275	37.0	37.0	37.0	33.0	37.0
13	35.133521159319784	37.0	37.0	37.0	33.0	37.0
14	35.10259922268938	37.0	37.0	37.0	33.0	37.0
15	35.13393437648997	37.0	37.0	37.0	33.0	37.0
16	35.12353748573245	37.0	37.0	37.0	33.0	37.0
17	35.095672778235304	37.0	37.0	37.0	33.0	37.0
18	35.113189718694464	37.0	37.0	37.0	33.0	37.0
19	35.12941210466242	37.0	37.0	37.0	33.0	37.0
20	35.116457890858655	37.0	37.0	37.0	33.0	37.0
21	35.12751939664514	37.0	37.0	37.0	33.0	37.0
22	35.1322323840897	37.0	37.0	37.0	33.0	37.0
23	35.106659153627206	37.0	37.0	37.0	33.0	37.0
24	35.09625070434745	37.0	37.0	37.0	33.0	37.0
25	35.08501582072732	37.0	37.0	37.0	33.0	37.0
26	35.05754699261699	37.0	37.0	37.0	33.0	37.0
27	34.99551529336974	37.0	37.0	37.0	27.0	37.0
28	34.9770332163033	37.0	37.0	37.0	27.0	37.0
29	35.00308612543886	37.0	37.0	37.0	27.0	37.0
30	34.961212488983286	37.0	37.0	37.0	27.0	37.0
31	34.96090329851329	37.0	37.0	37.0	27.0	37.0
32	34.97888835912329	37.0	37.0	37.0	27.0	37.0
33	34.98557785387138	37.0	37.0	37.0	27.0	37.0
34	34.897744643347345	37.0	37.0	37.0	27.0	37.0
35	34.89188158293962	37.0	37.0	37.0	27.0	37.0
36	34.867620244751706	37.0	37.0	37.0	27.0	37.0
37	34.90790747402944	37.0	37.0	37.0	27.0	37.0
38	34.78662967939549	37.0	37.0	37.0	27.0	37.0
39	34.84445407654631	37.0	37.0	37.0	27.0	37.0
40	34.86680825856414	37.0	37.0	37.0	27.0	37.0
41	34.89137300796093	37.0	37.0	37.0	27.0	37.0
42	34.80595263895511	37.0	37.0	37.0	27.0	37.0
43	34.59388265210293	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	4.0
16	21.0
17	25.0
18	21.0
19	27.0
20	36.0
21	86.0
22	266.0
23	564.0
24	1156.0
25	2095.0
26	3240.0
27	4691.0
28	6835.0
29	8809.0
30	11498.0
31	14333.0
32	18393.0
33	23658.0
34	32909.0
35	59430.0
36	157968.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.130134512302604	20.544695360698135	13.251267825408522	25.07390230159074
2	16.162281652290755	23.147385606750177	37.42042679843382	23.26990594252525
3	18.10122375854247	26.184387326080362	30.238539002788496	25.47584991258868
4	12.971840550185659	17.46983948102235	38.80687154147342	30.751448427318568
5	14.052851342955803	36.94681634952971	35.27169751347291	13.728634794041582
6	30.258766416713623	39.921979974860214	16.852325430193748	12.966928178232411
7	26.97961365639403	32.12402294366665	23.30429254619797	17.592070853741347
8	24.700273070087988	36.1877104012252	20.613468568043576	18.498547960643233
9	25.799777498446826	15.242223281753429	20.28087209050323	38.677127129296515
10	14.370132778524267	28.682761908889947	34.22680710271192	22.720298209873867
11	34.38718159883259	23.30978284426336	24.250068628725817	18.052966928178233
12	23.04306994350772	25.690549463251124	30.334763700460897	20.93161689278026
13	28.055712077210927	22.157398176643117	26.40371028563998	23.383179460505975
14	22.08457948651265	20.763151431089536	27.531533093493998	29.620735988903817
15	23.519570022972562	29.12487538468207	24.097496135119126	23.258058457226245
16	23.00232615260139	28.608209440423042	25.055697629058127	23.333766777917443
17	22.369497059800906	27.71415774493231	26.572175747330707	23.344169447936082
18	23.166457168451014	27.34948636816783	27.567653475503157	21.916402987878
19	24.373166890613035	26.573042636498922	27.102711918281248	21.95107855460679
20	23.899845404765	26.774738849638073	27.134497854449307	22.190917891147617
21	23.064453209657145	27.133342002225014	27.396587346307776	22.40561744181006
22	22.864201811798363	26.94262638521665	27.368846892924743	22.82432491006025
23	23.106352852787772	26.787453224105295	27.612442749194514	22.493751173912415
24	22.90263389825611	27.18593327843035	26.647017178853684	23.264415644459856
25	23.40485168971147	26.97383439527257	27.22263158655166	22.398682328464307
26	23.364396861861213	27.06659153627209	26.964587577478223	22.604424024388482
27	22.932108129975582	27.09606576799156	26.942048459104505	23.02977764292835
28	22.557034083192466	26.827041162787335	27.695953072399693	22.919971681620506
29	23.463511190094348	26.740641209021426	26.92326586045974	22.872581740424486
30	23.12715819282505	27.067169462384232	27.238813517691764	22.566858827098958
31	23.446751332842098	26.938869865487696	27.226388106280613	22.387990695389597
32	22.821435279499518	26.658864664152688	27.584991258867554	22.93470879748024
33	22.646034704463034	26.812593009983676	27.98289338708046	22.55847889847283
34	23.037579645442328	26.318755147154437	27.58441333275541	23.059251874647828
35	22.835305506191034	27.226677069336684	27.4676722581018	22.47034516637048
36	23.55106699608455	26.851025096441422	27.027870486758264	22.57003742071576
37	22.931241240807363	26.541834626443013	27.32232384089694	23.204600291852685
38	23.072255212171125	26.939158828543768	27.169462384234176	22.819123575050927
39	22.81738979671449	26.85275887477786	27.563030066605986	22.766821261901665
40	22.7697108924624	27.25412855966365	27.633537052287867	22.34262349558609
41	22.953491396125006	26.371346423359775	27.6159103058674	23.059251874647828
42	22.785892823602502	26.97961365639403	27.453513068354212	22.780980451649256
43	23.06127461604034	26.114169303454553	27.75027812694147	23.074277953563637
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	8.5
2	13.0
3	38.0
4	63.0
5	63.0
6	93.5
7	124.0
8	118.5
9	113.0
10	153.0
11	193.0
12	193.0
13	359.5
14	526.0
15	954.5
16	1383.0
17	1637.0
18	1891.0
19	1891.0
20	1990.5
21	2090.0
22	2569.5
23	3049.0
24	3995.0
25	4941.0
26	4941.0
27	6340.5
28	7740.0
29	9313.0
30	10886.0
31	12582.0
32	14278.0
33	14278.0
34	16417.0
35	18556.0
36	20182.0
37	21808.0
38	23284.5
39	24761.0
40	24761.0
41	25453.0
42	26145.0
43	26730.0
44	27315.0
45	27279.5
46	27244.0
47	27244.0
48	26196.0
49	25148.0
50	25012.0
51	24876.0
52	23870.0
53	22864.0
54	22864.0
55	21505.0
56	20146.0
57	17848.0
58	15550.0
59	14603.0
60	13656.0
61	13656.0
62	12252.0
63	10848.0
64	8983.0
65	7118.0
66	6015.5
67	4913.0
68	4913.0
69	4067.5
70	3222.0
71	2586.0
72	1950.0
73	1560.0
74	1170.0
75	1170.0
76	886.0
77	602.0
78	465.0
79	328.0
80	261.5
81	195.0
82	195.0
83	163.5
84	132.0
85	127.5
86	123.0
87	96.5
88	70.0
89	70.0
90	48.0
91	26.0
92	15.5
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	346065.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.50172344834564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.3358235228708	61.39491977068431
2	6.316818641364229	8.78059564570902
3	1.852515576745937	3.8625907589624595
4	0.9532999394336779	2.650239550153765
5	0.5555722384126428	1.9306614034866925
6	0.373376201734235	1.557017370907591
7	0.27928545479253036	1.3587574309495118
8	0.20727733892184483	1.1524905829484056
9	0.14986719978720495	0.9374425806229455
>10	0.9121220110932511	11.90157275313831
>50	0.046880326682993356	2.274675206136698
>100	0.016324399469887788	1.690515107150527
>500	4.1857434538173814E-4	0.21440537497331588
>1k	4.1857434538173814E-4	0.29411646417642107
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1011	0.2921416496900871	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	737	0.21296577232600813	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	396	0.1144293702050193	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.8896305607328105E-4	0.0	0.0
2	0.0	0.0	2.8896305607328105E-4	0.0	0.0
3	0.0	0.0	2.8896305607328105E-4	0.0	0.0
4	0.0	0.0	2.8896305607328105E-4	0.0	0.0
5	0.0	0.0	5.779261121465621E-4	0.0	0.0
6	0.0	0.0	8.668891682198431E-4	0.0	0.0
7	0.0	0.0	0.0023117044485862484	0.0	0.0
8	0.0	0.0	0.0028896305607328102	0.0	0.0
9	0.0	0.0	0.0031785936168060914	8.668891682198431E-4	0.0
10	0.0	0.0	0.0037565197289526533	8.668891682198431E-4	0.0
11	0.0	0.0	0.006646150289685464	8.668891682198431E-4	0.0
12	0.0	0.0	0.006935113345758745	8.668891682198431E-4	0.0
13	0.0	0.0	0.007224076401832025	8.668891682198431E-4	0.0
14	0.0	0.0	0.00837992862612515	0.0011558522242931242	0.0
15	0.0	0.0	0.008957854738271712	0.0011558522242931242	0.0
16	0.0	0.0	0.008957854738271712	0.0011558522242931242	0.0
17	0.0	0.0	0.009535780850418275	0.0011558522242931242	0.0
18	0.0	0.0	0.009824743906491556	0.0011558522242931242	0.0
19	0.0	0.0	0.009824743906491556	0.0011558522242931242	0.0
20	0.0	0.0	0.009824743906491556	0.0014448152803664051	0.0
21	0.0	0.0	0.010113706962564837	0.0014448152803664051	0.0
22	0.0	0.0	0.010402670018638116	0.0017337783364396863	0.0
23	0.0	0.0	0.010691633074711398	0.0017337783364396863	0.0
24	0.0	0.0	0.010980596130784679	0.002022741392512967	0.0
25	0.0	0.0	0.010980596130784679	0.002600667504659529	0.0
26	0.0	0.0	0.010980596130784679	0.002600667504659529	0.0
27	0.0	0.0	0.010980596130784679	0.004912371953245778	0.0
28	0.0	0.0	0.010980596130784679	0.010980596130784679	0.0
29	0.0	0.0	0.010980596130784679	0.021961192261569357	0.0
30	0.0	0.0	0.010980596130784679	0.03929897562596622	0.0
31	0.0	0.0	0.010980596130784679	0.09073439960701024	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGCC	25	0.005491103	29.599998	27
CGCGGCG	25	0.005491103	29.599998	27
GCCGGCA	25	0.005491103	29.599998	15
TGGTCGA	40	5.9283222E-5	27.75	10
GGTCGAT	35	8.8568195E-4	26.428572	11
CCTACCG	40	0.0019284758	23.125	24
CGATTGG	40	0.0019284758	23.125	14
TCGATAC	40	0.0019284758	23.125	1
GTATCAA	550	0.0	22.872726	1
GGTATCA	205	0.0	22.560976	1
GTCGATT	45	0.003820488	20.555555	12
CGTGCAA	45	0.003820488	20.555555	11
AGACGAT	45	0.003820488	20.555555	29
CTTATGG	45	0.003820488	20.555555	1
CTACCGA	45	0.003820488	20.555555	25
GTATCAG	45	0.003820488	20.555555	1
CGGAGTC	55	5.1331177E-4	20.181818	27
CTCTAGT	120	2.3646862E-10	20.041666	27
TGAACTT	130	3.274181E-11	19.923077	37
ACACTTA	65	6.884326E-5	19.923077	6
>>END_MODULE