FastQCFastQC Report
Fri 10 Feb 2017
ERR1632879.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632879.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92255
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC3410.36962766245731943No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3310.35878814156414285No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT2860.3100102975448485No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2760.299170776651672No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.27098802232941305No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC2320.25147688472169527No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC2300.24930898054305997No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG2060.22329413039943635No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2010.21787436995284806No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA1940.2102867053276245No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA1890.20486694488103624No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA1860.2016150886130833No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG1750.18969161563058912No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1720.18643975936263615No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG1530.1658446696656008No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC1530.1658446696656008No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT1530.1658446696656008No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC1520.16476071757628313No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT1510.16367676548696547No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC1500.1625928133976478No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG1500.1625928133976478No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA1420.1539211966831066No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC1390.15066934041515365No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG1380.149585388325836No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT1290.13982981952197712No Hit
GATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGAT1280.1387458674326595No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA1280.1387458674326595No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC1260.13657796325402416No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1250.13549401116470652No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT1250.13549401116470652No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG1250.13549401116470652No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT1240.13441005907538886No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT1200.13007425071811826No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG1190.1289902986288006No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC1180.12790634653948296No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT1150.12465449027152999No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG1150.12465449027152999No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC1140.12357053818221235No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA1130.1224865860928947No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG1130.1224865860928947No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT1060.11489892146767113No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATT1030.11164706519971818No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG1020.11056311311040051No Hit
ACCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGA1010.10947916102108286No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT1010.10947916102108286No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGG1000.10839520893176523No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA990.10731125684244756No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGC980.10622730475312991No Hit
GGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCG950.10297544848517695No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT940.1018914963958593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA950.031.1578961
TCCTTTT250.005470529.619
TGCCGAG250.005470529.611
CCTTTTA250.005470529.620
CCCTGTC250.005470529.637
CCGAGTT250.005470529.613
GCCGAGT250.005470529.612
AGTTCCT250.005470529.616
TTTGCCG250.005470529.69
TTGCCGA250.005470529.610
GGTTTAG358.8074506E-426.4285736
GTATACC358.8074506E-426.428571
GTTTAGA358.8074506E-426.4285737
AAGTATA601.3152694E-624.6666685
GGATTTA601.3152694E-624.6666682
ATAAAGT601.3152694E-624.6666682
ACCTGTG451.3103697E-424.6666668
TATACCC400.001917827423.1252
GTTTAGG400.001917827423.1251
TTTAGGA400.001917827423.1252
Produced by FastQC (version 0.11.5)