##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632879.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	92255
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.23132621538128	33.0	27.0	33.0	14.0	33.0
2	29.480049861796108	33.0	27.0	33.0	14.0	33.0
3	29.957248929597313	33.0	33.0	33.0	14.0	33.0
4	30.093306595848464	33.0	33.0	33.0	14.0	33.0
5	30.20867161671454	33.0	33.0	33.0	14.0	33.0
6	30.729846620779362	37.0	27.0	37.0	14.0	37.0
7	31.206742181995555	37.0	27.0	37.0	14.0	37.0
8	31.391252506639205	37.0	27.0	37.0	14.0	37.0
9	30.92547829385941	37.0	27.0	37.0	14.0	37.0
10	30.909587556230015	37.0	27.0	37.0	14.0	37.0
11	31.135580727331853	37.0	27.0	37.0	14.0	37.0
12	31.29156143298466	37.0	27.0	37.0	14.0	37.0
13	31.36659259660723	37.0	27.0	37.0	14.0	37.0
14	31.242501761422144	37.0	27.0	37.0	14.0	37.0
15	31.455856051162538	37.0	27.0	37.0	14.0	37.0
16	31.47175762831283	37.0	27.0	37.0	14.0	37.0
17	31.62111538669991	37.0	27.0	37.0	14.0	37.0
18	31.612064386754106	37.0	27.0	37.0	14.0	37.0
19	31.61623760229798	37.0	27.0	37.0	14.0	37.0
20	31.507170343070836	37.0	27.0	37.0	14.0	37.0
21	31.612823153216628	37.0	27.0	37.0	14.0	37.0
22	31.56757899300851	37.0	27.0	37.0	14.0	37.0
23	31.54730908893827	37.0	27.0	37.0	14.0	37.0
24	31.532101241125144	37.0	27.0	37.0	14.0	37.0
25	31.555471248170832	37.0	27.0	37.0	14.0	37.0
26	31.432464365075063	37.0	27.0	37.0	14.0	37.0
27	31.369172402579807	37.0	27.0	37.0	14.0	37.0
28	31.328383285458784	37.0	27.0	37.0	14.0	37.0
29	31.39228226112406	37.0	27.0	37.0	14.0	37.0
30	31.317944826838655	37.0	27.0	37.0	14.0	37.0
31	31.259097067909597	37.0	27.0	37.0	14.0	37.0
32	31.283420952793886	37.0	27.0	37.0	14.0	37.0
33	31.262327245135765	37.0	27.0	37.0	14.0	37.0
34	31.16621321337597	37.0	27.0	37.0	14.0	37.0
35	31.150257438621214	37.0	27.0	37.0	14.0	37.0
36	31.14363449135548	37.0	27.0	37.0	14.0	37.0
37	31.146842989539863	37.0	27.0	37.0	14.0	37.0
38	31.05755785594277	37.0	27.0	37.0	14.0	37.0
39	31.106032193377054	37.0	27.0	37.0	14.0	37.0
40	31.09932252994418	37.0	27.0	37.0	14.0	37.0
41	31.19170776651672	37.0	27.0	37.0	14.0	37.0
42	30.949238523657254	37.0	27.0	37.0	14.0	37.0
43	30.665991003197657	37.0	27.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	4.0
16	10.0
17	23.0
18	50.0
19	129.0
20	314.0
21	647.0
22	1138.0
23	1774.0
24	2725.0
25	3503.0
26	4319.0
27	5228.0
28	6213.0
29	6980.0
30	7770.0
31	8369.0
32	8906.0
33	9196.0
34	9150.0
35	8650.0
36	7157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.711560349032574	25.254999729011978	10.309468321500189	22.723971600455258
2	18.57351905045797	26.400737087420733	32.77437537260853	22.251368489512764
3	19.32144599208715	29.710042816107528	29.046664137445127	21.921847054360196
4	14.865318952902282	24.322800932198795	35.987209365346054	24.82467074955287
5	16.146550322475747	38.264592705002435	34.80786949216845	10.78098748035337
6	27.804455043087096	41.757086336783914	17.43428540458512	13.004173215543874
7	26.92103409029321	32.97382255704298	24.225245244160206	15.879898108503603
8	23.654002493089806	36.766570917565446	20.484526583924993	19.09490000541976
9	25.81323505501057	15.48425559590266	21.28773508210937	37.4147742669774
10	16.368760500785864	28.248875399707334	34.0989648257547	21.2833992737521
11	33.56024063736383	24.439867757845104	24.417104763969434	17.582786840821633
12	22.89957183892472	27.809874803533685	28.671616714541216	20.618936643000378
13	27.01967373042112	23.173811717522085	26.125413256734053	23.681101295322744
14	21.799360468267302	21.771177713945043	27.52154354777519	28.907918270012466
15	24.727115061514283	28.95236030567449	25.38290607555146	20.937618557259768
16	22.85187794699474	28.494932523982442	25.854425234404637	22.79876429461818
17	22.01723483822015	28.468917673838817	26.2695788846133	23.244268603327733
18	21.66603436128123	28.699799468863475	28.553465936805594	21.080700233049697
19	24.955828952360307	26.402904991599367	28.21852474120644	20.422741314833885
20	23.363503333152675	26.79095983957509	28.95561216194244	20.889924665329794
21	22.051921305078316	28.75182916915072	28.491680667714487	20.704568858056476
22	21.977128610915397	28.143732047043517	27.50094845807815	22.37819088396293
23	22.315321662782505	27.07278738279768	29.933336946506966	20.67855400791285
24	22.39228226112406	29.135548208769173	25.228984878868353	23.243184651238415
25	23.108774592163027	28.21744078911712	27.97355156902065	20.700233049699204
26	22.51693675139559	28.63693024768305	28.69437970841689	20.15175329250447
27	23.13045363394938	27.365454446913446	26.60235217603382	22.901739743103356
28	21.63459975069102	27.977887377377918	29.729553953715243	20.657958918215815
29	24.419272668148068	27.66462522356512	26.725922714216033	21.190179394070782
30	23.212833992737522	26.52322367351363	28.858056473903854	21.405885859844993
31	22.756490163134792	27.800119234729827	27.790363665925966	21.653026936209418
32	21.42648094954203	27.588748577312884	27.598504146116742	23.386266327028345
33	21.10346322692537	28.133976478239664	28.47000162592813	22.292558668906835
34	22.57655411630806	26.95355265297274	28.843965096742725	21.625928133976476
35	22.824779144761802	28.209853124491897	27.808790851444364	21.156576879301937
36	24.152620454175924	28.21852474120644	25.88477589290553	21.7440789117121
37	22.342420465015447	27.194190016801258	27.991978754539048	22.471410763644247
38	22.3640995068018	27.636442469242862	27.814210611890957	22.18524741206439
39	21.605333044279444	27.7925315701046	28.51335970950084	22.088775676115116
40	21.958701425397	28.645601864397598	28.90466641374451	20.491030296460895
41	22.13213375968782	27.043520676386102	27.80662294726573	23.017722616660343
42	21.412389572380903	28.35510270446046	28.256463064332554	21.97604465882608
43	23.293046447347027	26.050620562571137	27.74266977399599	22.91366321608585
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	6.0
2	3.0
3	13.5
4	24.0
5	24.0
6	32.5
7	41.0
8	54.5
9	68.0
10	84.5
11	101.0
12	101.0
13	161.5
14	222.0
15	393.0
16	564.0
17	524.0
18	484.0
19	484.0
20	444.0
21	404.0
22	309.0
23	214.0
24	308.0
25	402.0
26	402.0
27	626.0
28	850.0
29	1260.0
30	1670.0
31	2414.0
32	3158.0
33	3158.0
34	4303.0
35	5448.0
36	6299.0
37	7150.0
38	7720.5
39	8291.0
40	8291.0
41	9130.0
42	9969.0
43	10005.5
44	10042.0
45	9586.0
46	9130.0
47	9130.0
48	8723.5
49	8317.0
50	7910.5
51	7504.0
52	6557.0
53	5610.0
54	5610.0
55	5404.5
56	5199.0
57	3911.5
58	2624.0
59	2335.0
60	2046.0
61	2046.0
62	1794.0
63	1542.0
64	1077.0
65	612.0
66	454.5
67	297.0
68	297.0
69	205.0
70	113.0
71	93.0
72	73.0
73	57.5
74	42.0
75	42.0
76	27.0
77	12.0
78	9.5
79	7.0
80	5.5
81	4.0
82	4.0
83	4.0
84	4.0
85	3.5
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	92255.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.986504796488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.59704729335223	30.642241612920706
2	7.670920566074457	5.981247628854804
3	3.186254066227375	3.7266272830740883
4	2.024077626713376	3.156468484093003
5	1.351238635415798	2.634003577041895
6	1.0787666472043818	2.5234404639314945
7	0.7923930269413629	2.162484418188716
8	0.6088914838602052	1.8990840604845265
9	0.5338226707815498	1.8730692103409028
>10	3.672811188033475	28.420139829819526
>50	0.35866210693135375	9.176738388163242
>100	0.12511468846442572	7.804455043087096
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	341	0.36962766245731943	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	331	0.35878814156414285	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	286	0.3100102975448485	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	276	0.299170776651672	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	250	0.27098802232941305	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	232	0.25147688472169527	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	230	0.24930898054305997	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	206	0.22329413039943635	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	201	0.21787436995284806	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	194	0.2102867053276245	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	189	0.20486694488103624	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	186	0.2016150886130833	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	175	0.18969161563058912	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	172	0.18643975936263615	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	153	0.1658446696656008	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	153	0.1658446696656008	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	153	0.1658446696656008	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	152	0.16476071757628313	No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT	151	0.16367676548696547	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	150	0.1625928133976478	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	150	0.1625928133976478	No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA	142	0.1539211966831066	No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC	139	0.15066934041515365	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	138	0.149585388325836	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	129	0.13982981952197712	No Hit
GATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGAT	128	0.1387458674326595	No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA	128	0.1387458674326595	No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC	126	0.13657796325402416	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	125	0.13549401116470652	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	125	0.13549401116470652	No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG	125	0.13549401116470652	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	124	0.13441005907538886	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	120	0.13007425071811826	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	119	0.1289902986288006	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	118	0.12790634653948296	No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT	115	0.12465449027152999	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	115	0.12465449027152999	No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC	114	0.12357053818221235	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	113	0.1224865860928947	No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG	113	0.1224865860928947	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT	106	0.11489892146767113	No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATT	103	0.11164706519971818	No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG	102	0.11056311311040051	No Hit
ACCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGA	101	0.10947916102108286	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	101	0.10947916102108286	No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGG	100	0.10839520893176523	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	99	0.10731125684244756	No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGC	98	0.10622730475312991	No Hit
GGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCG	95	0.10297544848517695	No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT	94	0.1018914963958593	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0010839520893176522	0.0	0.0
5	0.0	0.0	0.0021679041786353045	0.0	0.0
6	0.0	0.0	0.0021679041786353045	0.0	0.0
7	0.0	0.0	0.007587664625223565	0.0010839520893176522	0.0
8	0.0	0.0	0.00975556880385887	0.0010839520893176522	0.0
9	0.0	0.0	0.010839520893176522	0.0010839520893176522	0.0
10	0.0010839520893176522	0.0	0.013007425071811825	0.0010839520893176522	0.0
11	0.0010839520893176522	0.0	0.016259281339764783	0.0010839520893176522	0.0
12	0.0010839520893176522	0.0	0.016259281339764783	0.0010839520893176522	0.0
13	0.0010839520893176522	0.0	0.018427185518400085	0.0010839520893176522	0.0
14	0.0010839520893176522	0.0	0.018427185518400085	0.0010839520893176522	0.0
15	0.0010839520893176522	0.0	0.01951113760771774	0.0010839520893176522	0.0
16	0.0010839520893176522	0.0	0.01951113760771774	0.0010839520893176522	0.0
17	0.0010839520893176522	0.0	0.01951113760771774	0.0010839520893176522	0.0
18	0.0010839520893176522	0.0	0.02059508969703539	0.0010839520893176522	0.0
19	0.0010839520893176522	0.0	0.02059508969703539	0.0010839520893176522	0.0
20	0.0010839520893176522	0.0	0.02059508969703539	0.0010839520893176522	0.0
21	0.0010839520893176522	0.0	0.02059508969703539	0.0010839520893176522	0.0
22	0.0010839520893176522	0.0	0.02059508969703539	0.0021679041786353045	0.0
23	0.0010839520893176522	0.0	0.02059508969703539	0.0021679041786353045	0.0
24	0.0010839520893176522	0.0	0.02059508969703539	0.0032518562679529563	0.0
25	0.0010839520893176522	0.0	0.02059508969703539	0.004335808357270609	0.0
26	0.0010839520893176522	0.0	0.02059508969703539	0.004335808357270609	0.0
27	0.0010839520893176522	0.0	0.02059508969703539	0.005419760446588261	0.0
28	0.0010839520893176522	0.0	0.02059508969703539	0.011923472982494174	0.0
29	0.0010839520893176522	0.0	0.02059508969703539	0.022762993875670694	0.0
30	0.0010839520893176522	0.0	0.02059508969703539	0.03902227521543548	0.0
31	0.0010839520893176522	0.0	0.02059508969703539	0.09430383177063574	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	95	0.0	31.157896	1
TCCTTTT	25	0.0054705	29.6	19
TGCCGAG	25	0.0054705	29.6	11
CCTTTTA	25	0.0054705	29.6	20
CCCTGTC	25	0.0054705	29.6	37
CCGAGTT	25	0.0054705	29.6	13
GCCGAGT	25	0.0054705	29.6	12
AGTTCCT	25	0.0054705	29.6	16
TTTGCCG	25	0.0054705	29.6	9
TTGCCGA	25	0.0054705	29.6	10
GGTTTAG	35	8.8074506E-4	26.42857	36
GTATACC	35	8.8074506E-4	26.42857	1
GTTTAGA	35	8.8074506E-4	26.42857	37
AAGTATA	60	1.3152694E-6	24.666668	5
GGATTTA	60	1.3152694E-6	24.666668	2
ATAAAGT	60	1.3152694E-6	24.666668	2
ACCTGTG	45	1.3103697E-4	24.666666	8
TATACCC	40	0.0019178274	23.125	2
GTTTAGG	40	0.0019178274	23.125	1
TTTAGGA	40	0.0019178274	23.125	2
>>END_MODULE