FastQCFastQC Report
Fri 10 Feb 2017
ERR1632878.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632878.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391089
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86692.21663099703648No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67551.72722832910156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33460.8555597319280265No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA20980.5364507823027496No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG20140.5149722953087404No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18830.4814760834490359No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18100.46281025546614707No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14920.3814988404173986No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG14920.3814988404173986No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT13500.34518996954657377No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC11300.28893678932416916No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT11080.2833114713019287No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC11070.2830557750281905No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10990.2810102048382849No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG10750.27487349426856805No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC10120.25876462902306124No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.2516051333583916No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9830.2513494370846534No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9730.24879247434727134No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9700.2480253855260567No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA9530.24367854887250728No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA9440.24137728240886344No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC8980.22961525381690612No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8930.2283367724482151No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA8780.22450132834214206No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC8770.22424563206840387No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8670.22168866933102183No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG8620.2204101879623308No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8530.21810892149868702No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT8200.2096709444653263No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT8150.20839246309663528No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8010.20481271526430045No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7670.19611904195720153No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7520.1922835978511285No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC7400.1892152425662701No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7270.18589119100767346No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG7270.18589119100767346No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6870.17566334005814532No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6820.17438485868945433No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT6780.1733620735945015No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6730.1720835922258105No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6600.16875954066721385No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6590.16850384439347565No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA6420.1641570077399262No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6170.15776460089647115No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6080.1554633344328273No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.15469624561161271No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC5970.15265067542170707No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5900.15086080150553965No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT5810.14855953504189584No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5710.1460025723045138No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5700.1457468760307756No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC5620.14370130584087No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5590.1429342170196554No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5530.14140003937722617No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5440.13909877291358233No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5440.13909877291358233No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5400.13807598781862954No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA5390.1378202915448913No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5330.1362861139024621No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5300.1355190250812475No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5260.1344962399862947No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5180.13245066979638906No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC5130.13117218842769804No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC5100.13040509960648344No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5090.13014940333274524No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG4920.1258025666791958No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4840.12375699648929017No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4820.12324560394181376No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4820.12324560394181376No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4800.12273421139433736No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.12222281884686095No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA4770.12196712257312274No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4720.12068864120443172No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4650.11889876728826432No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4620.1181316784670497No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4610.1178759821933115No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4610.1178759821933115No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4590.1173645896458351No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4580.11710889337209689No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA4570.11685319709835869No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC4490.11480762690845306No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG4480.11455193063471486No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT4330.11071648652864181No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4320.11046079025490363No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4300.10994939770742722No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4290.109693701433689No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4260.10892661261247441No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT4210.10764813124378339No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4180.10688104242256877No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG4160.10636964987509237No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4110.10509116850640136No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4080.10432407968518674No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG4060.10381268713771034No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG3930.1004886355791137No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC3930.1004886355791137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTA200.001840451637.05
AACCCGG200.001840451637.013
TAGGTAG200.001840451637.06
AAACCCG200.001840451637.012
CTCGTAT401.5964961E-632.37536
TCGTATG401.5964961E-632.37537
TAACATT352.3843095E-531.71428530
ACTAGTG601.2660166E-930.8333328
GGAAATT303.595175E-430.83333219
GAGATTG303.595175E-430.8333329
GGGGACA250.00549196529.59999818
TAGACCC250.00549196529.5999984
CTATTTA250.00549196529.5999981
TGATCTA250.00549196529.59999820
CTCCGGT453.99937E-628.77777937
CATTCTC453.99937E-628.77777933
ATTCTCC453.99937E-628.77777934
GAATCAA706.5647328E-926.428571
CATACTA1200.026.2083325
TCATACT1150.025.739134
Produced by FastQC (version 0.11.5)