FastQCFastQC Report
Fri 10 Feb 2017
ERR1632877.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632877.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380643
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42101.106023229114945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31890.8377928925528645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15950.4190278029544744No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10920.2868829848440665No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9870.25929808245521396No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9850.2587726557430453No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8960.23539116705154173No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7640.2007130040484128No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7210.1894163297367875No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG6530.17155182152305443No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5910.15526359344582719No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5830.1531618865971527No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5360.14081435886119015No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5210.1368736585199255No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5110.13424652495908238No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4920.1292549711934805No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4870.12794140441305896No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4830.1268905509887217No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4790.12583969756438448No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4640.12189899722311982No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4640.12189899722311982No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG4620.12137357051095121No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4590.12058543044269826No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4580.12032271708661396No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4510.1184837235940238No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4220.11086503626757882No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4180.10981418284324157No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4080.10718704928239847No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4010.10534805578980831No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3910.1027209222289652No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3850.10114464209245934No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3820.1003565020242064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCT1850.026.028
CGCTCTC1800.025.69444529
AGCTTCG2100.025.54761921
GCCGGCA2100.025.54761915
CTGCTCG2550.024.6666669
ATCTCGT451.3209417E-424.66666637
CACGAGA451.3209417E-424.66666621
CTAGTCC451.3209417E-424.6666669
GGTATCA6600.024.6666661
TGCTCGC2350.024.40425710
TTCGCCG2050.024.36585424
GCTTCGC2150.024.09302322
GCCGCTC2000.024.05000127
GTATCAA17950.023.601671
GTATTAG551.8983272E-523.5454541
CTCCGGC400.001928827523.12527
ACCCGTC652.675024E-622.769237
CGGCAGC2300.022.5217417
CCGGCAG2400.022.35416816
ATCTCCG502.6975025E-422.210
Produced by FastQC (version 0.11.5)