##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632877.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	380643
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.757801929892313	33.0	33.0	33.0	27.0	33.0
2	31.238420252047195	33.0	33.0	33.0	27.0	33.0
3	31.70023880644068	33.0	33.0	33.0	27.0	33.0
4	31.809682563451844	33.0	33.0	33.0	27.0	33.0
5	31.94836894412875	33.0	33.0	33.0	33.0	33.0
6	34.83112260044189	37.0	37.0	37.0	27.0	37.0
7	35.00200713004048	37.0	37.0	37.0	33.0	37.0
8	35.19669086256676	37.0	37.0	37.0	33.0	37.0
9	35.30157916998342	37.0	37.0	37.0	33.0	37.0
10	35.31021455799792	37.0	37.0	37.0	33.0	37.0
11	35.348702064664266	37.0	37.0	37.0	33.0	37.0
12	35.302806041356334	37.0	37.0	37.0	33.0	37.0
13	35.320468260285885	37.0	37.0	37.0	33.0	37.0
14	35.29502184461556	37.0	37.0	37.0	33.0	37.0
15	35.3146439051815	37.0	37.0	37.0	33.0	37.0
16	35.30747445769396	37.0	37.0	37.0	33.0	37.0
17	35.277414795490785	37.0	37.0	37.0	33.0	37.0
18	35.29294378196893	37.0	37.0	37.0	33.0	37.0
19	35.2874845984295	37.0	37.0	37.0	33.0	37.0
20	35.28461839571462	37.0	37.0	37.0	33.0	37.0
21	35.29344031021193	37.0	37.0	37.0	33.0	37.0
22	35.29800364120712	37.0	37.0	37.0	33.0	37.0
23	35.269916956308144	37.0	37.0	37.0	33.0	37.0
24	35.26726617854525	37.0	37.0	37.0	33.0	37.0
25	35.25343432034741	37.0	37.0	37.0	33.0	37.0
26	35.22215304103845	37.0	37.0	37.0	33.0	37.0
27	35.156671737034436	37.0	37.0	37.0	33.0	37.0
28	35.115622775146264	37.0	37.0	37.0	33.0	37.0
29	35.15263908701854	37.0	37.0	37.0	33.0	37.0
30	35.10939909574063	37.0	37.0	37.0	33.0	37.0
31	35.108106546028694	37.0	37.0	37.0	33.0	37.0
32	35.11363928930783	37.0	37.0	37.0	33.0	37.0
33	35.078204511839175	37.0	37.0	37.0	33.0	37.0
34	35.003819852197466	37.0	37.0	37.0	27.0	37.0
35	34.96638845322257	37.0	37.0	37.0	27.0	37.0
36	34.936880489067185	37.0	37.0	37.0	27.0	37.0
37	34.93050443591502	37.0	37.0	37.0	27.0	37.0
38	34.76619036735209	37.0	37.0	37.0	27.0	37.0
39	34.80945400283205	37.0	37.0	37.0	27.0	37.0
40	34.80237125075202	37.0	37.0	37.0	27.0	37.0
41	34.80173023016317	37.0	37.0	37.0	27.0	37.0
42	34.66800125051557	37.0	37.0	37.0	27.0	37.0
43	34.41997357103638	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	7.0
16	21.0
17	25.0
18	16.0
19	10.0
20	35.0
21	89.0
22	223.0
23	492.0
24	1010.0
25	1847.0
26	3040.0
27	4610.0
28	6772.0
29	9104.0
30	11760.0
31	15502.0
32	19276.0
33	25197.0
34	35831.0
35	63971.0
36	181802.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.53685737029185	18.775860845989552	13.891756843026142	23.79552494069246
2	16.457940905257683	23.910593390657386	37.429034554687725	22.202431149397203
3	19.40322034031888	26.729770414798114	30.270621028102447	23.596388216780554
4	12.988022898096116	17.881321868522473	38.18696258699096	30.943692646390446
5	15.14910296524565	36.11625591433443	32.81841515540809	15.916225965011835
6	28.7242902141902	39.04971324837183	17.17514836736785	15.050848170070118
7	26.36039543614358	32.60246477670678	21.5461731858986	19.490966601251042
8	25.306389451533327	33.6217925983139	20.37893774481601	20.69288020533676
9	27.505300241959002	14.200970463137374	18.863344393565626	39.430384901338
10	16.222287024850058	27.17848482699012	31.581035248250988	25.018192899908836
11	35.192555754341996	23.499446988385444	22.70211195266956	18.605885304603
12	23.948161400577444	24.98798086395914	29.354802268792547	21.709055466670872
13	30.57930922150151	21.686987544759788	25.480568406617227	22.253134827121475
14	23.340242694598352	21.13870477061183	28.141854703751285	27.37919783103853
15	25.757205570574005	26.62783763263741	25.00137924511944	22.613577551669152
16	22.160922439135884	27.569665014199657	25.178710760476353	25.090701786188106
17	23.044690169003502	25.71595957366877	25.522339830234632	25.7170104270931
18	24.80749678832922	23.839660784514624	27.712318366553433	23.640524060602715
19	26.409785547087427	24.817217182504343	27.675538496701634	21.097458773706595
20	26.414514387496947	23.337352847681423	27.648741734381034	22.599391030440596
21	24.25448517377175	25.229414438200624	27.53945297824996	22.976647409777666
22	23.639735920534463	25.156905551921355	27.694453858339703	23.508904669204476
23	23.630540953071513	25.329770940224833	28.03676936131756	23.002918745386097
24	23.817592862603544	24.810386635246147	28.132134309576163	23.239886192574144
25	23.74666025646078	25.410949367254883	28.59503524299672	22.247355133287623
26	24.11419624162273	25.228889011488455	27.531308864211347	23.12560588267747
27	24.2368833789141	25.04446423551727	28.331533746844155	22.387118638724473
28	23.298471270980944	25.631103159653534	27.401265752949616	23.669159816415906
29	23.781075706107824	25.7070273195619	26.72766870794944	23.784228266380836
30	23.91006796394522	25.583552042202275	27.97976056304726	22.52661943080524
31	24.276815809038915	24.916785544460296	27.484545886828343	23.32185275967245
32	22.40629671371863	25.29377921044128	28.134498729780923	24.16542534605917
33	22.936189552940682	25.275389275515376	28.33179646020024	23.4566247113437
34	22.196126028851182	25.603780970620765	27.781411979203614	24.418681021324442
35	23.005283165590857	25.81237537535171	27.902260123002392	23.280081336055044
36	23.327107026794135	25.401754399791933	28.120312208552374	23.15082636486156
37	24.306765131632527	25.402805253216272	27.230764784850898	23.059664830300306
38	23.67914292394711	25.70939173976666	26.918135891110566	23.693329445175664
39	23.20179275594192	25.019769180045344	27.69970812546139	24.078729938551348
40	23.200479189161495	25.86912146026592	27.96189605483353	22.96850329573905
41	23.61635443184296	25.095430626597626	27.949811240453652	23.33840370110576
42	23.189970654918127	25.7706039517343	27.67501306998946	23.36441232335811
43	23.028927367638445	25.843638264725737	27.600665190217605	23.52676917741821
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	32.5
2	59.0
3	247.5
4	436.0
5	436.0
6	561.5
7	687.0
8	682.5
9	678.0
10	893.0
11	1108.0
12	1108.0
13	1721.5
14	2335.0
15	3991.0
16	5647.0
17	5981.0
18	6315.0
19	6315.0
20	5046.0
21	3777.0
22	3248.5
23	2720.0
24	3062.5
25	3405.0
26	3405.0
27	4123.0
28	4841.0
29	6166.5
30	7492.0
31	8231.0
32	8970.0
33	8970.0
34	10610.0
35	12250.0
36	13082.0
37	13914.0
38	15710.0
39	17506.0
40	17506.0
41	19681.0
42	21856.0
43	22755.0
44	23654.0
45	26942.0
46	30230.0
47	30230.0
48	32800.5
49	35371.0
50	35972.0
51	36573.0
52	36697.5
53	36822.0
54	36822.0
55	32789.5
56	28757.0
57	26099.5
58	23442.0
59	20659.0
60	17876.0
61	17876.0
62	15087.5
63	12299.0
64	9737.0
65	7175.0
66	6342.0
67	5509.0
68	5509.0
69	4563.0
70	3617.0
71	2945.5
72	2274.0
73	1869.5
74	1465.0
75	1465.0
76	1051.0
77	637.0
78	498.0
79	359.0
80	286.5
81	214.0
82	214.0
83	182.0
84	150.0
85	135.0
86	120.0
87	91.5
88	63.0
89	63.0
90	46.5
91	30.0
92	15.5
93	1.0
94	1.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	380643.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.89057400182187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.70312207678319	44.47184198312491
2	7.649591861302582	7.938834251253117
3	2.2447119513907157	3.4943817487924176
4	1.0513636274772318	2.1822344845772466
5	0.6183719144278841	1.6043836793134185
6	0.3931434695301442	1.2240264179392164
7	0.29528062326457943	1.0725596722970319
8	0.22024885910322667	0.9143071777690257
9	0.18148850043447437	0.8475788216047294
>10	1.3532849711616586	14.092608285261138
>50	0.1695376991790016	6.071009230693865
>100	0.11217146864109154	11.346264953082951
>500	0.005634183356401858	2.059284630041853
>1k	0.0020487939477824937	2.6806846642491133
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4210	1.106023229114945	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3189	0.8377928925528645	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1595	0.4190278029544744	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1092	0.2868829848440665	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	987	0.25929808245521396	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	985	0.2587726557430453	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	896	0.23539116705154173	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	764	0.2007130040484128	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	721	0.1894163297367875	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	653	0.17155182152305443	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	591	0.15526359344582719	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	583	0.1531618865971527	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	536	0.14081435886119015	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	521	0.1368736585199255	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	511	0.13424652495908238	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	492	0.1292549711934805	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	487	0.12794140441305896	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	483	0.1268905509887217	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	479	0.12583969756438448	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	464	0.12189899722311982	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	464	0.12189899722311982	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	462	0.12137357051095121	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	459	0.12058543044269826	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	458	0.12032271708661396	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	451	0.1184837235940238	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	422	0.11086503626757882	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	418	0.10981418284324157	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	408	0.10718704928239847	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	401	0.10534805578980831	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	391	0.1027209222289652	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	385	0.10114464209245934	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	382	0.1003565020242064	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	7.881400682529299E-4	0.0	0.0
2	0.0	0.0	7.881400682529299E-4	0.0	0.0
3	0.0	0.0	0.0015762801365058598	0.0	0.0
4	0.0	0.0	0.0018389934925901697	0.0	0.0
5	0.0	0.0	0.0036779869851803395	0.0	0.0
6	0.0	0.0	0.00866954075078223	0.0	0.0
7	0.0	0.0	0.019178074994154626	0.0	0.0
8	0.0	0.0	0.022067921911082037	0.0	0.0
9	0.0	0.0	0.027322189032768236	7.881400682529299E-4	0.0
10	0.0015762801365058598	0.0	0.031525602730117196	7.881400682529299E-4	0.0
11	0.0015762801365058598	0.0	0.051491817792524756	7.881400682529299E-4	0.0
12	0.0015762801365058598	0.0	0.056220658202042334	7.881400682529299E-4	0.0
13	0.0015762801365058598	0.0	0.059110505118969744	7.881400682529299E-4	0.0
14	0.0015762801365058598	0.0	0.06252577874806577	7.881400682529299E-4	0.0
15	0.0015762801365058598	0.0	0.06804275922583629	7.881400682529299E-4	0.0
16	0.0021017068486744797	0.0	0.07487330648402835	7.881400682529299E-4	0.0
17	0.0021017068486744797	0.0	0.0761868732644499	7.881400682529299E-4	0.0
18	0.0021017068486744797	0.0	0.07802586675704005	7.881400682529299E-4	0.0
19	0.0021017068486744797	0.0	0.07933943353746162	0.0010508534243372399	0.0
20	0.0021017068486744797	0.0	0.08091571367396747	0.0010508534243372399	0.0
21	0.0021017068486744797	0.0	0.08380556059089488	0.0013135667804215498	0.0
22	0.0021017068486744797	0.0	0.08538184072740074	0.0018389934925901697	0.0
23	0.0021017068486744797	0.0	0.08722083421999091	0.0021017068486744797	0.0
24	0.0021017068486744797	0.0	0.08853440100041246	0.0031525602730117196	0.0
25	0.0023644202047587897	0.0	0.08879711435649677	0.0031525602730117196	0.0
26	0.0023644202047587897	0.0	0.08879711435649677	0.0039407003412646495	0.0
27	0.0026271335608430996	0.0	0.08879711435649677	0.005779693833854819	0.0
28	0.0026271335608430996	0.0	0.08879711435649677	0.01655094143331153	0.0
29	0.0026271335608430996	0.0	0.08905982771258109	0.055695231489873716	0.0
30	0.0026271335608430996	0.0	0.08905982771258109	0.10167006880462796	0.0
31	0.0026271335608430996	0.0	0.08905982771258109	0.2193656523303988	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCTCT	185	0.0	26.0	28
CGCTCTC	180	0.0	25.694445	29
AGCTTCG	210	0.0	25.547619	21
GCCGGCA	210	0.0	25.547619	15
CTGCTCG	255	0.0	24.666666	9
ATCTCGT	45	1.3209417E-4	24.666666	37
CACGAGA	45	1.3209417E-4	24.666666	21
CTAGTCC	45	1.3209417E-4	24.666666	9
GGTATCA	660	0.0	24.666666	1
TGCTCGC	235	0.0	24.404257	10
TTCGCCG	205	0.0	24.365854	24
GCTTCGC	215	0.0	24.093023	22
GCCGCTC	200	0.0	24.050001	27
GTATCAA	1795	0.0	23.60167	1
GTATTAG	55	1.8983272E-5	23.545454	1
CTCCGGC	40	0.0019288275	23.125	27
ACCCGTC	65	2.675024E-6	22.76923	7
CGGCAGC	230	0.0	22.52174	17
CCGGCAG	240	0.0	22.354168	16
ATCTCCG	50	2.6975025E-4	22.2	10
>>END_MODULE