FastQCFastQC Report
Fri 10 Feb 2017
ERR1632876.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632876.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356995
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8680.2431406602333366No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC5870.16442807322231404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5790.162187145478228No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT5040.14117844787742126No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC4710.1319346209330663No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA4620.1294135772209695No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG4550.12745276544489417No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4190.11736859059650695No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA4000.11204638720430259No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT3990.11176627123629182No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC3860.108124763652152No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3630.1016820963879046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3590.10056163251586157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2100.029.9523831
CTTCCGG250.005491330329.622
CCTAGTC250.005491330329.62
CTTCGGT400.001928593623.12500212
GCTAACT400.001928593623.1250022
GTATCAA6000.023.1251
CGAACCG502.6970476E-422.231
GCGAACC502.6970476E-422.230
CTAGTAA502.6970476E-422.230
AACCGGG555.133553E-420.18181833
GAGTAGT555.133553E-420.1818181
CCCTTGA555.133553E-420.1818181
ATTGCCG555.133553E-420.18181811
GGCGAAC555.133553E-420.18181829
TCCTATA656.885106E-519.9230772
TATTGTC951.6680497E-719.4736845
TTAACGG951.6680497E-719.47368435
GCACCGC1052.246452E-819.38095310
GTCGCCC902.14377E-618.537
ACAGTCC500.00702561518.58