##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632876.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	356995
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.444244317147298	33.0	33.0	33.0	27.0	33.0
2	31.04162243168672	33.0	33.0	33.0	27.0	33.0
3	31.50756173055645	33.0	33.0	33.0	27.0	33.0
4	31.620403647109903	33.0	33.0	33.0	27.0	33.0
5	31.76898556002185	33.0	33.0	33.0	27.0	33.0
6	34.56984271488396	37.0	37.0	37.0	27.0	37.0
7	34.75979495511142	37.0	37.0	37.0	27.0	37.0
8	34.915679491309405	37.0	37.0	37.0	27.0	37.0
9	34.992621745402595	37.0	37.0	37.0	27.0	37.0
10	35.047098698861326	37.0	37.0	37.0	27.0	37.0
11	35.06271236291825	37.0	37.0	37.0	33.0	37.0
12	35.01797784282693	37.0	37.0	37.0	27.0	37.0
13	35.05585792518102	37.0	37.0	37.0	27.0	37.0
14	35.00915138867491	37.0	37.0	37.0	27.0	37.0
15	35.0400621857449	37.0	37.0	37.0	27.0	37.0
16	35.021173966021934	37.0	37.0	37.0	27.0	37.0
17	35.01507864255802	37.0	37.0	37.0	27.0	37.0
18	34.99580106163952	37.0	37.0	37.0	27.0	37.0
19	35.001448199554616	37.0	37.0	37.0	27.0	37.0
20	34.987943808736816	37.0	37.0	37.0	27.0	37.0
21	35.011890922842056	37.0	37.0	37.0	27.0	37.0
22	35.014666872085044	37.0	37.0	37.0	27.0	37.0
23	35.007523914900766	37.0	37.0	37.0	27.0	37.0
24	34.98366643790529	37.0	37.0	37.0	27.0	37.0
25	34.98217341979579	37.0	37.0	37.0	27.0	37.0
26	34.946506253588986	37.0	37.0	37.0	27.0	37.0
27	34.88107676578103	37.0	37.0	37.0	27.0	37.0
28	34.859953220633344	37.0	37.0	37.0	27.0	37.0
29	34.90123110967941	37.0	37.0	37.0	27.0	37.0
30	34.85199792714184	37.0	37.0	37.0	27.0	37.0
31	34.82660821580134	37.0	37.0	37.0	27.0	37.0
32	34.85974593481701	37.0	37.0	37.0	27.0	37.0
33	34.83756355131024	37.0	37.0	37.0	27.0	37.0
34	34.7584167845488	37.0	37.0	37.0	27.0	37.0
35	34.78169162033082	37.0	37.0	37.0	27.0	37.0
36	34.74118405019678	37.0	37.0	37.0	27.0	37.0
37	34.783271474390396	37.0	37.0	37.0	27.0	37.0
38	34.64787181893304	37.0	37.0	37.0	27.0	37.0
39	34.71358702502836	37.0	37.0	37.0	27.0	37.0
40	34.75913388142691	37.0	37.0	37.0	27.0	37.0
41	34.78336111150016	37.0	37.0	37.0	27.0	37.0
42	34.69098446756957	37.0	37.0	37.0	27.0	37.0
43	34.46007086933991	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	6.0
16	20.0
17	21.0
18	17.0
19	22.0
20	41.0
21	127.0
22	280.0
23	694.0
24	1332.0
25	2420.0
26	3773.0
27	5389.0
28	7298.0
29	9825.0
30	12442.0
31	15356.0
32	19591.0
33	25023.0
34	34935.0
35	62063.0
36	156319.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.927183854115604	21.106458073642486	13.308589756159051	24.65776831608286
2	16.02039244247118	23.44430594266026	38.25403717138896	22.2812644434796
3	17.627417750948894	27.20122130562053	31.0651409683609	24.106219975069678
4	12.917547864816035	18.21902267538761	39.15909186403171	29.704337595764645
5	13.41167243238701	37.7447863415454	36.08117760752952	12.762363618538075
6	29.13486183279878	40.10868499558817	17.546744352161795	13.209708819451253
7	25.783274275550077	32.72538831076066	24.13927365929495	17.35206375439432
8	23.46167313267693	37.07278813428759	20.89216935811426	18.573369374921217
9	25.52472723707615	16.01843163069511	20.34818414823737	38.108656983991374
10	14.415888177705572	28.76538887099259	34.70748890040476	22.11123405089707
11	32.972450594546146	24.284093614756507	24.556926567598985	18.186529223098365
12	22.2734211963753	26.307371251698203	30.434599924368687	20.984607627557807
13	27.055280886286926	22.560540063586323	27.281334472471602	23.102844577655148
14	21.631395397694643	21.71206879648174	27.68105995882295	28.97547584700066
15	23.508172383366713	29.334304402022436	25.07262006470679	22.08490314990406
16	22.793316433003262	29.341867533158727	25.221921875656523	22.642894158181488
17	22.2837854871917	28.263140940349306	26.45863387442401	22.994439698034984
18	21.816271936581742	28.275466042941776	28.05837616773344	21.849885852743036
19	24.261684337315646	26.784128629252514	28.125884116024036	20.828302917407807
20	23.817980643986612	26.611577192957885	28.316643090239356	21.253799072816147
21	22.1145954425132	28.13988991442457	27.3334360425216	22.412078600540625
22	21.73391784198658	28.104315186487206	27.21074524853289	22.95102172299332
23	22.11403521057718	27.741004775977256	28.12028179666382	22.024678216781748
24	22.9636269415538	28.327007381055758	26.435664365047128	22.27370131234331
25	23.123853275255957	27.376573901595258	27.64296418717349	21.856608635975295
26	22.573425398114818	27.85109035140548	27.245759744534237	22.32972450594546
27	22.597515371363745	27.69590610512752	26.796453731844984	22.91012479166375
28	21.835599938374486	27.741845123881287	28.11299878149554	22.309556156248686
29	23.22553537164386	27.784702866986933	27.026989173517833	21.96277258785137
30	22.38854885922772	27.9505315200493	27.410748049692575	22.250171571030407
31	22.834493480300843	27.508508522528324	27.655569405733978	22.001428591436856
32	21.728315522626367	27.611591198756287	27.69198448157537	22.968108797041975
33	22.0807014103839	27.30710514152859	28.267902911805486	22.34429053628202
34	22.461659126878526	27.18133307189176	27.772937996330484	22.58406980489923
35	22.653258448997885	27.924760850992307	27.274331573271333	22.147649126738468
36	22.98827714673875	27.769296488746342	26.970965979915682	22.271460384599223
37	22.912365719407834	26.90121710388101	27.19617921819633	22.990237958514825
38	23.005084104819396	27.59954621213182	27.291138531351976	22.1042311516968
39	22.614322329444388	27.444361965853865	27.62447653328478	22.316839171416966
40	22.479306432863204	27.500665275424023	27.76453451729016	22.25549377442261
41	22.043445986638467	27.325032563481283	27.95809465118559	22.67342679869466
42	22.416280340060784	27.366769842714884	27.65444894186193	22.562500875362403
43	22.61908430090057	26.641549601535036	27.785543214890968	22.95382288267343
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	16.0
2	26.0
3	61.5
4	97.0
5	97.0
6	123.0
7	149.0
8	141.0
9	133.0
10	191.5
11	250.0
12	250.0
13	373.0
14	496.0
15	933.5
16	1371.0
17	1628.5
18	1886.0
19	1886.0
20	2019.0
21	2152.0
22	2678.5
23	3205.0
24	4176.0
25	5147.0
26	5147.0
27	6623.5
28	8100.0
29	10839.5
30	13579.0
31	15101.5
32	16624.0
33	16624.0
34	18350.0
35	20076.0
36	21811.0
37	23546.0
38	25624.0
39	27702.0
40	27702.0
41	29416.0
42	31130.0
43	30069.0
44	29008.0
45	28209.5
46	27411.0
47	27411.0
48	26441.0
49	25471.0
50	25826.0
51	26181.0
52	25634.0
53	25087.0
54	25087.0
55	22277.0
56	19467.0
57	16788.5
58	14110.0
59	12334.0
60	10558.0
61	10558.0
62	9490.0
63	8422.0
64	6877.0
65	5332.0
66	4508.0
67	3684.0
68	3684.0
69	2982.5
70	2281.0
71	2032.5
72	1784.0
73	1436.0
74	1088.0
75	1088.0
76	836.5
77	585.0
78	459.5
79	334.0
80	258.0
81	182.0
82	182.0
83	145.5
84	109.0
85	115.5
86	122.0
87	92.5
88	63.0
89	63.0
90	44.5
91	26.0
92	19.5
93	13.0
94	7.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	356995.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.95211927168111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.98673263534172	51.56969743280523
2	5.511917426663724	6.388545922513476
3	1.6507646928577968	2.869959371099253
4	0.8104227628237012	1.8786286644657784
5	0.5011854909072939	1.4522380673147772
6	0.35621856698096727	1.2386172528281005
7	0.25145537563057135	1.0200660344033774
8	0.2152115882904389	0.9977574106604357
9	0.15600096472931044	0.8136527863041285
>10	1.3003452779831797	15.563344091478607
>50	0.1777204121730141	7.137057708098396
>100	0.08007183405597297	8.35231551242292
>500	0.001952971562340804	0.7181197456055208
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	868	0.2431406602333366	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	587	0.16442807322231404	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	579	0.162187145478228	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	504	0.14117844787742126	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	471	0.1319346209330663	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	462	0.1294135772209695	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	455	0.12745276544489417	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	419	0.11736859059650695	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	400	0.11204638720430259	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	399	0.11176627123629182	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	386	0.108124763652152	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	363	0.1016820963879046	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	359	0.10056163251586157	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	2.8011596801075646E-4	0.0	0.0
6	0.0	0.0	5.602319360215129E-4	0.0	0.0
7	0.0	0.0	0.0014005798400537823	0.0	0.0
8	0.0	0.0	0.0014005798400537823	0.0	0.0
9	0.0	0.0	0.0016806958080645387	0.0	0.0
10	0.0	0.0	0.0022409277440860517	0.0	0.0
11	0.0	0.0	0.003081275648118321	0.0	0.0
12	0.0	0.0	0.00392162355215059	0.0	0.0
13	0.0	0.0	0.004481855488172103	0.0	0.0
14	0.0	0.0	0.004481855488172103	0.0	0.0
15	0.0	0.0	0.0053222033922043725	0.0	0.0
16	0.0	0.0	0.006722783232258155	0.0	0.0
17	0.0	0.0	0.006722783232258155	0.0	0.0
18	0.0	0.0	0.007002899200268912	0.0	0.0
19	0.0	0.0	0.007283015168279667	0.0	0.0
20	0.0	0.0	0.007283015168279667	0.0	0.0
21	0.0	0.0	0.007283015168279667	2.8011596801075646E-4	2.8011596801075646E-4
22	0.0	0.0	0.007283015168279667	2.8011596801075646E-4	2.8011596801075646E-4
23	0.0	0.0	0.007283015168279667	5.602319360215129E-4	2.8011596801075646E-4
24	0.0	0.0	0.007283015168279667	5.602319360215129E-4	2.8011596801075646E-4
25	0.0	0.0	0.007283015168279667	5.602319360215129E-4	2.8011596801075646E-4
26	0.0	0.0	0.007283015168279667	8.403479040322694E-4	2.8011596801075646E-4
27	0.0	0.0	0.007283015168279667	0.0011204638720430258	2.8011596801075646E-4
28	0.0	0.0	0.007283015168279667	0.005602319360215129	2.8011596801075646E-4
29	0.0	0.0	0.007283015168279667	0.015126262272580848	2.8011596801075646E-4
30	0.0	0.0	0.007283015168279667	0.0327735682572585	2.8011596801075646E-4
31	0.0	0.0	0.007283015168279667	0.08543537024328071	2.8011596801075646E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	210	0.0	29.952383	1
CTTCCGG	25	0.0054913303	29.6	22
CCTAGTC	25	0.0054913303	29.6	2
CTTCGGT	40	0.0019285936	23.125002	12
GCTAACT	40	0.0019285936	23.125002	2
GTATCAA	600	0.0	23.125	1
CGAACCG	50	2.6970476E-4	22.2	31
GCGAACC	50	2.6970476E-4	22.2	30
CTAGTAA	50	2.6970476E-4	22.2	30
AACCGGG	55	5.133553E-4	20.181818	33
GAGTAGT	55	5.133553E-4	20.181818	1
CCCTTGA	55	5.133553E-4	20.181818	1
ATTGCCG	55	5.133553E-4	20.181818	11
GGCGAAC	55	5.133553E-4	20.181818	29
TCCTATA	65	6.885106E-5	19.923077	2
TATTGTC	95	1.6680497E-7	19.473684	5
TTAACGG	95	1.6680497E-7	19.473684	35
GCACCGC	105	2.246452E-8	19.380953	10
GTCGCCC	90	2.14377E-6	18.5	37
ACAGTCC	50	0.007025615	18.5	8
>>END_MODULE