FastQCFastQC Report
Fri 10 Feb 2017
ERR1632875.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632875.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491605
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25620.5211501103528239No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18930.3850652454714659No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG12040.2449120737177205No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC10970.22314663195044804No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG10810.2198919864525381No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC10730.21826466370358316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9890.20117777483955615No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA9660.19649922193631067No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG9480.19283774575116203No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8700.1769713489488512No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8440.1716825500147476No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT8350.1698518119221733No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8310.1690381505476958No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT8220.1672074124551215No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA7990.16252885955187601No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG7930.16130836749015978No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA7830.15927421405396608No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG7820.1590707987103467No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT7790.1584605526794886No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7550.15357858443262376No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC7460.15174784634004942No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7390.15032393893471385No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG7370.14991710824747512No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT7240.1472727087804233No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7140.1452385553442296No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC7060.14361123259527467No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG7010.14259415587717783No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT6780.13791560297393232No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC6540.13303363472706745No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA6520.1326268040398287No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA6490.1320165580089706No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT6150.12510043632591206No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT6080.12367652892057647No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG6080.12367652892057647No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG6050.12306628288971837No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT6030.12265945220247963No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG5940.12082871410990531No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5940.12082871410990531No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC5860.11920139136095036No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG5700.11594674586304045No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC5650.11492966914494361No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5530.11248868502151117No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA5490.11167502364703369No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA5460.1110647776161756No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT5430.11045453158531747No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG5410.11004770089807873No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA5380.10943745486722063No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.10719988608740758No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG5250.10679305540016884No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTAC5230.1063862247129301No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC5210.10597939402569136No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC5210.10597939402569136No Hit
ACCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGA5200.10577597868207197No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT5160.1049623173075945No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGC5070.10313157921502018No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG5010.10191108715330396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATAGC200.001840911237.09
GCCGGCA900.034.94444715
GCCGCTC850.034.8235327
GGCAGCT950.033.10526318
CCGGCAG950.033.10526316
CCGCTCT900.032.8888928
AGCTTCG1000.031.4521
CGGCAGC1000.031.4517
CAGCTTC1000.031.4520
CGCTCTC900.030.83333429
ATACCAT303.5965093E-430.8333326
GCAGCTT1050.029.9523819
GGGCGTT250.005493321529.632
CCGCCTA250.005493321529.615
GTGCGGG250.005493321529.69
GGTATCA4550.028.4615381
GCTCTCT1050.028.19047530
TTCGCCG1100.026.9090924
CTTAACG358.8621484E-426.4285717
TACTTTG358.8621484E-426.428577
Produced by FastQC (version 0.11.5)