FastQCFastQC Report
Fri 10 Feb 2017
ERR1632874.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632874.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335135
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9420.28108075850030584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7150.21334686022050817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3860.11517746579736525No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTTA200.00184007637.023
GTGTGCG250.005490857629.5999989
CGCAATA250.005490857629.59999834
TCTATCG250.005490857629.59999831
CCGTCCG405.9277692E-527.7528
GGTATCA1600.025.43751
CGAGTCG451.3204811E-424.66666621
GCCGTCC451.3204811E-424.66666627
TTGTCCG551.897449E-523.54545413
TGTCCGG551.897449E-523.54545414
GTATCAA4950.023.1717171
AACGGCC400.001928348723.12537
TTAACGG400.001928348723.12535
TAAGGCC400.001928348723.1254
AGTCGCC502.6965706E-422.19999923
GGCGAAC603.7161535E-521.58333429
CGAGCGA450.003820238920.55555513
TCCGGCG555.1326514E-420.18181816
TCGCCGT555.1326514E-420.18181825
GTCCGGC555.1326514E-420.18181815
Produced by FastQC (version 0.11.5)