##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632874.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	335135
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.749954495949392	33.0	33.0	33.0	27.0	33.0
2	31.26100825040655	33.0	33.0	33.0	27.0	33.0
3	31.70974980231847	33.0	33.0	33.0	27.0	33.0
4	31.844444775985796	33.0	33.0	33.0	27.0	33.0
5	31.957703611977262	33.0	33.0	33.0	33.0	33.0
6	34.863267041639936	37.0	37.0	37.0	27.0	37.0
7	35.030539931669324	37.0	37.0	37.0	33.0	37.0
8	35.217216942426184	37.0	37.0	37.0	33.0	37.0
9	35.30480254225909	37.0	37.0	37.0	33.0	37.0
10	35.345830784609184	37.0	37.0	37.0	33.0	37.0
11	35.356480224387184	37.0	37.0	37.0	33.0	37.0
12	35.301606815164035	37.0	37.0	37.0	33.0	37.0
13	35.346469333253765	37.0	37.0	37.0	33.0	37.0
14	35.312647142196425	37.0	37.0	37.0	33.0	37.0
15	35.33448013487102	37.0	37.0	37.0	33.0	37.0
16	35.32333238844048	37.0	37.0	37.0	33.0	37.0
17	35.30188133140376	37.0	37.0	37.0	33.0	37.0
18	35.29757261998896	37.0	37.0	37.0	33.0	37.0
19	35.3168872245513	37.0	37.0	37.0	33.0	37.0
20	35.31269488415116	37.0	37.0	37.0	33.0	37.0
21	35.29841407194116	37.0	37.0	37.0	33.0	37.0
22	35.31623674041804	37.0	37.0	37.0	33.0	37.0
23	35.309869157205306	37.0	37.0	37.0	33.0	37.0
24	35.29030390738061	37.0	37.0	37.0	33.0	37.0
25	35.29657899055604	37.0	37.0	37.0	33.0	37.0
26	35.25670550673609	37.0	37.0	37.0	33.0	37.0
27	35.20211258149701	37.0	37.0	37.0	33.0	37.0
28	35.18329926745938	37.0	37.0	37.0	33.0	37.0
29	35.215381861041074	37.0	37.0	37.0	33.0	37.0
30	35.17848628164769	37.0	37.0	37.0	33.0	37.0
31	35.17292136004894	37.0	37.0	37.0	33.0	37.0
32	35.197222015008876	37.0	37.0	37.0	33.0	37.0
33	35.185546123204084	37.0	37.0	37.0	33.0	37.0
34	35.13482924791502	37.0	37.0	37.0	33.0	37.0
35	35.11634117594402	37.0	37.0	37.0	33.0	37.0
36	35.10226923478598	37.0	37.0	37.0	33.0	37.0
37	35.13309561818372	37.0	37.0	37.0	33.0	37.0
38	34.99195548062721	37.0	37.0	37.0	27.0	37.0
39	35.06687454309457	37.0	37.0	37.0	27.0	37.0
40	35.10587673624062	37.0	37.0	37.0	33.0	37.0
41	35.117272144061346	37.0	37.0	37.0	33.0	37.0
42	35.02897041490743	37.0	37.0	37.0	27.0	37.0
43	34.822032912110046	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	7.0
16	9.0
17	20.0
18	10.0
19	11.0
20	15.0
21	66.0
22	177.0
23	408.0
24	859.0
25	1561.0
26	2558.0
27	4030.0
28	5356.0
29	7478.0
30	9817.0
31	12621.0
32	16437.0
33	21339.0
34	30317.0
35	56610.0
36	165429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.48000059677444	19.835887030599608	13.24481179226282	25.43930058036314
2	16.321482387694513	22.80036403240485	37.37389410237665	23.504259477523984
3	18.408104196816208	25.9960911274561	29.830366867083413	25.765437808644275
4	13.123666582123622	17.508466737284976	38.39169289987617	30.976173780715232
5	14.709594640965582	36.338192071851644	34.69676399063064	14.255449296552136
6	30.64496396974354	38.636668805108386	17.327644083727453	13.39072314142062
7	27.089381890879793	31.872528980858462	22.95403344920704	18.084055679054707
8	24.932937472958656	35.28727229325496	20.590209915407222	19.18958031837916
9	26.431736464409866	15.193280319871096	19.728467632446627	38.64651558327241
10	15.301296492458263	27.522043355662646	33.620779685798254	23.555880466080833
11	34.679755919256415	23.024154445223566	23.931549972399182	18.364539663120834
12	23.406388470317932	24.77240515016337	30.251391230399687	21.569815149119012
13	28.623688961164905	21.620242588807496	25.800647500261086	23.955420949766513
14	22.44677518015128	20.44191146851269	27.441777194265	29.66953615707103
15	24.45044534292151	28.102704880122936	23.73401763468453	23.712832142271026
16	23.60481596968386	27.781938621749447	24.857147119817387	23.75609828874931
17	23.013114118191176	26.939591507899802	26.109179882733823	23.938114491175195
18	23.731928924164887	26.6940188282334	27.009414116699244	22.564638130902473
19	25.011711698270844	26.07576051441956	26.553180061766152	22.359347725543437
20	24.676026079042774	25.990123383114266	26.585107493994958	22.748743043848002
21	23.85874349142883	26.585405881212047	26.500067137123846	23.055783490235278
22	23.40967072970594	26.484252614617994	26.475002610888147	23.631074044787923
23	23.398630402673547	26.139018604442988	27.056260909782626	23.40609008310084
24	23.809211213391617	26.51021230250496	26.119921822549124	23.5606546615543
25	23.84979187491608	26.177510555447803	26.763841437032838	23.208856132603277
26	23.809807987825803	26.38369612245811	26.503051009294758	23.303444880421324
27	23.73909021737509	26.365792889432615	26.437107434317515	23.45800945887478
28	23.370582004266936	26.428155817804765	26.634042997597984	23.567219180330316
29	24.182493621973233	26.365494502215526	26.172139585540155	23.279872290271083
30	23.80413863070106	26.306115446014296	26.65701881331404	23.232727109970607
31	24.01927581422412	26.00146209736375	26.41920420129202	23.560057887120113
32	23.193638384531607	26.133349247318243	26.84052695182538	23.832485416324765
33	23.215719038596387	26.088889551971594	27.172035150014175	23.523356259417845
34	23.4430900980202	25.955510465931642	26.835454369134826	23.765945066913332
35	23.504557864741074	26.461575186119024	26.585405881212047	23.448461067927852
36	23.925283840840258	26.341325137631106	26.39921225774688	23.33417876378176
37	23.639727274083576	26.123800856371314	26.397421934444328	23.839049935100782
38	23.61973533053844	26.157220224685574	26.69222850493085	23.530815939845137
39	23.344323929162876	26.115446014292747	26.85365598937742	23.686574067166962
40	23.293896489474392	26.54661554299014	26.829188237575902	23.33029972995957
41	23.55319498112701	25.653542602234918	26.950333447715103	23.842928968922973
42	23.187372252972683	26.510809076939147	26.95928506422785	23.342533605860325
43	23.628388559834097	25.727841019290732	26.888567293777133	23.755203127098035
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	7.5
2	12.0
3	61.0
4	110.0
5	110.0
6	128.5
7	147.0
8	141.5
9	136.0
10	190.5
11	245.0
12	245.0
13	391.0
14	537.0
15	942.0
16	1347.0
17	1552.0
18	1757.0
19	1757.0
20	1681.0
21	1605.0
22	1848.5
23	2092.0
24	2834.5
25	3577.0
26	3577.0
27	4846.5
28	6116.0
29	7444.0
30	8772.0
31	10439.0
32	12106.0
33	12106.0
34	14145.5
35	16185.0
36	17618.5
37	19052.0
38	20654.5
39	22257.0
40	22257.0
41	23244.0
42	24231.0
43	25072.0
44	25913.0
45	26080.5
46	26248.0
47	26248.0
48	26034.0
49	25820.0
50	25459.0
51	25098.0
52	24375.5
53	23653.0
54	23653.0
55	22508.0
56	21363.0
57	19465.5
58	17568.0
59	16131.0
60	14694.0
61	14694.0
62	13030.0
63	11366.0
64	9574.0
65	7782.0
66	6707.5
67	5633.0
68	5633.0
69	4703.5
70	3774.0
71	3145.0
72	2516.0
73	1972.5
74	1429.0
75	1429.0
76	1125.5
77	822.0
78	650.5
79	479.0
80	377.0
81	275.0
82	275.0
83	220.5
84	166.0
85	157.5
86	149.0
87	104.5
88	60.0
89	60.0
90	45.0
91	30.0
92	18.5
93	7.0
94	5.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	335135.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.54968315686618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.60483850434203	66.80012319820861
2	6.291183592550252	9.38011487012593
3	1.6452956545723894	3.6796880924322117
4	0.7455603684081201	2.2232515695656714
5	0.39653494873334627	1.4780777394347568
6	0.24026003995454964	1.074678591232061
7	0.18742237172117143	0.9780594899825209
8	0.12304550347408402	0.7338402638296033
9	0.1059225624053146	0.7106844119831629
>10	0.59917792575326	8.354958681627744
>50	0.04265168196737311	2.2389671913412017
>100	0.017302097401764478	1.8505075835111764
>500	8.047487163611385E-4	0.49704831672524225
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	942	0.28108075850030584	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	715	0.21334686022050817	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	386	0.11517746579736525	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.983872170916198E-4	0.0	0.0	0.0
3	0.0	2.983872170916198E-4	0.0	0.0	0.0
4	0.0	2.983872170916198E-4	0.0	2.983872170916198E-4	0.0
5	0.0	2.983872170916198E-4	5.967744341832396E-4	2.983872170916198E-4	0.0
6	0.0	2.983872170916198E-4	0.0020887105196413388	2.983872170916198E-4	0.0
7	0.0	2.983872170916198E-4	0.004774195473465917	2.983872170916198E-4	0.0
8	0.0	2.983872170916198E-4	0.005967744341832396	2.983872170916198E-4	0.0
9	0.0	2.983872170916198E-4	0.007161293210198875	5.967744341832396E-4	0.0
10	5.967744341832396E-4	2.983872170916198E-4	0.008653229295656975	5.967744341832396E-4	0.0
11	5.967744341832396E-4	2.983872170916198E-4	0.011637101466573173	8.951616512748593E-4	0.0
12	5.967744341832396E-4	2.983872170916198E-4	0.012532263117848032	8.951616512748593E-4	0.0
13	5.967744341832396E-4	2.983872170916198E-4	0.013129037552031271	0.0011935488683664792	0.0
14	5.967744341832396E-4	2.983872170916198E-4	0.01491936085458099	0.0011935488683664792	0.0
15	5.967744341832396E-4	2.983872170916198E-4	0.016112909722947467	0.0011935488683664792	0.0
16	5.967744341832396E-4	2.983872170916198E-4	0.01730645859131395	0.001491936085458099	0.0
17	5.967744341832396E-4	2.983872170916198E-4	0.01790323302549719	0.0017903233025497187	0.0
18	5.967744341832396E-4	2.983872170916198E-4	0.018500007459680428	0.0017903233025497187	0.0
19	5.967744341832396E-4	2.983872170916198E-4	0.019096781893863667	0.0017903233025497187	0.0
20	5.967744341832396E-4	2.983872170916198E-4	0.019693556328046907	0.0017903233025497187	0.0
21	5.967744341832396E-4	2.983872170916198E-4	0.020887105196413385	0.0020887105196413388	0.0
22	5.967744341832396E-4	2.983872170916198E-4	0.020887105196413385	0.0020887105196413388	0.0
23	5.967744341832396E-4	2.983872170916198E-4	0.020887105196413385	0.0020887105196413388	0.0
24	5.967744341832396E-4	2.983872170916198E-4	0.020887105196413385	0.0023870977367329584	0.0
25	5.967744341832396E-4	2.983872170916198E-4	0.020887105196413385	0.0023870977367329584	0.0
26	5.967744341832396E-4	2.983872170916198E-4	0.020887105196413385	0.0023870977367329584	0.0
27	5.967744341832396E-4	2.983872170916198E-4	0.020887105196413385	0.0050725826905575365	0.0
28	5.967744341832396E-4	2.983872170916198E-4	0.020887105196413385	0.01521774807167261	0.0
29	5.967744341832396E-4	2.983872170916198E-4	0.020887105196413385	0.02804839840661226	0.0
30	5.967744341832396E-4	2.983872170916198E-4	0.020887105196413385	0.052814537425216704	0.0
31	5.967744341832396E-4	2.983872170916198E-4	0.020887105196413385	0.12860489056648813	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCTTA	20	0.001840076	37.0	23
GTGTGCG	25	0.0054908576	29.599998	9
CGCAATA	25	0.0054908576	29.599998	34
TCTATCG	25	0.0054908576	29.599998	31
CCGTCCG	40	5.9277692E-5	27.75	28
GGTATCA	160	0.0	25.4375	1
CGAGTCG	45	1.3204811E-4	24.666666	21
GCCGTCC	45	1.3204811E-4	24.666666	27
TTGTCCG	55	1.897449E-5	23.545454	13
TGTCCGG	55	1.897449E-5	23.545454	14
GTATCAA	495	0.0	23.171717	1
AACGGCC	40	0.0019283487	23.125	37
TTAACGG	40	0.0019283487	23.125	35
TAAGGCC	40	0.0019283487	23.125	4
AGTCGCC	50	2.6965706E-4	22.199999	23
GGCGAAC	60	3.7161535E-5	21.583334	29
CGAGCGA	45	0.0038202389	20.555555	13
TCCGGCG	55	5.1326514E-4	20.181818	16
TCGCCGT	55	5.1326514E-4	20.181818	25
GTCCGGC	55	5.1326514E-4	20.181818	15
>>END_MODULE