FastQCFastQC Report
Fri 10 Feb 2017
ERR1632873.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632873.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34968
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC550.15728666209105469No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT500.14298787462823154No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC440.12582932967284374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT380.10867078471745596No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG360.10295126973232671No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT360.10295126973232671No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT350.10009151223976206No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC800.00613899213.8754
ATCAACG800.00613899213.8753
CAACGCA850.0091683413.0588245
Produced by FastQC (version 0.11.5)