##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632873.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34968
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.579758636467627	33.0	27.0	33.0	14.0	33.0
2	29.193205216197665	33.0	27.0	33.0	14.0	33.0
3	29.732498284145503	33.0	27.0	33.0	14.0	33.0
4	29.837079615648594	33.0	27.0	33.0	14.0	33.0
5	29.96556851978952	33.0	33.0	33.0	14.0	33.0
6	29.627001830244794	33.0	27.0	37.0	14.0	37.0
7	30.24691146190803	33.0	27.0	37.0	14.0	37.0
8	30.1570006863418	33.0	27.0	37.0	14.0	37.0
9	29.702013269274765	33.0	27.0	37.0	14.0	37.0
10	29.69657973003889	33.0	27.0	37.0	14.0	37.0
11	29.84248455730954	33.0	27.0	37.0	14.0	37.0
12	30.089739190116678	33.0	27.0	37.0	14.0	37.0
13	30.426847403340197	33.0	27.0	37.0	14.0	37.0
14	30.244337680164723	33.0	27.0	37.0	14.0	37.0
15	30.548558682223746	37.0	27.0	37.0	14.0	37.0
16	30.58224662548616	37.0	27.0	37.0	14.0	37.0
17	30.533544955387782	37.0	27.0	37.0	14.0	37.0
18	30.512096774193548	37.0	27.0	37.0	14.0	37.0
19	30.531714710592542	37.0	27.0	37.0	14.0	37.0
20	30.548787462823153	37.0	27.0	37.0	14.0	37.0
21	30.532086479066574	37.0	27.0	37.0	14.0	37.0
22	30.597517730496453	37.0	27.0	37.0	14.0	37.0
23	30.54381148478609	37.0	27.0	37.0	14.0	37.0
24	30.551904598490047	37.0	27.0	37.0	14.0	37.0
25	30.382635552505146	37.0	27.0	37.0	14.0	37.0
26	30.354352550903684	37.0	27.0	37.0	14.0	37.0
27	30.37242621825669	37.0	27.0	37.0	14.0	37.0
28	30.229524136353238	33.0	27.0	37.0	14.0	37.0
29	30.28297300388927	33.0	27.0	37.0	14.0	37.0
30	30.256320064058567	33.0	27.0	37.0	14.0	37.0
31	30.241763898421414	33.0	27.0	37.0	14.0	37.0
32	30.241620910546786	33.0	27.0	37.0	14.0	37.0
33	30.126000915122397	33.0	27.0	37.0	14.0	37.0
34	30.09654541294898	33.0	27.0	37.0	14.0	37.0
35	30.13395104095173	33.0	27.0	37.0	14.0	37.0
36	30.12256920613132	33.0	27.0	37.0	14.0	37.0
37	30.107240905971175	33.0	27.0	37.0	14.0	37.0
38	30.111673530084648	33.0	27.0	37.0	14.0	37.0
39	30.122311827956988	33.0	27.0	37.0	14.0	37.0
40	30.103408830931137	33.0	27.0	37.0	14.0	37.0
41	30.138641043239534	33.0	27.0	37.0	14.0	37.0
42	30.00511896591169	33.0	27.0	37.0	14.0	37.0
43	29.561198810340883	33.0	27.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	5.0
16	7.0
17	19.0
18	29.0
19	101.0
20	226.0
21	457.0
22	761.0
23	1090.0
24	1442.0
25	1795.0
26	2218.0
27	2504.0
28	2778.0
29	2941.0
30	2918.0
31	2996.0
32	2903.0
33	2680.0
34	2457.0
35	2279.0
36	2361.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.34145504461222	23.34420041180508	8.156028368794328	23.15831617478838
2	16.400709219858157	29.69858156028369	30.78528940745825	23.11541981239991
3	20.23278425989476	29.955959734614506	27.5308853809197	22.280370624571034
4	16.56943491191947	26.121024937085334	33.21894303363075	24.09059711736445
5	18.405399222145963	37.41706703271562	32.27236330359185	11.905170441546558
6	28.6547700754976	41.22340425531915	17.378746282315262	12.743079386867995
7	26.461336078700526	33.53923587279798	23.409974834134065	16.589453214367424
8	23.9247311827957	37.5886524822695	19.963967055593685	18.52264927934111
9	25.626286890871654	16.992679020819036	20.258522077327843	37.12251201098147
10	15.225348890414093	30.09608785175017	32.83287577213452	21.845687485701212
11	33.404827270647445	24.622512010981467	24.239304506977806	17.733356211393275
12	22.815145275680624	26.51853122855182	30.2962708762297	20.370052619537862
13	27.445092656142762	23.123999084877603	26.398421413864103	23.032486845115535
14	21.54255319148936	22.654998856097002	26.898878975062914	28.90356897735072
15	23.384237016700986	29.22958133150309	24.533859528711965	22.85232212308396
16	22.697895218485474	29.326813086250286	25.697780828185767	22.27751086707847
17	21.5396934339968	29.547014413177763	26.312628689087163	22.600663463738275
18	22.020132692747655	29.14378860672615	27.479409746053534	21.35666895447266
19	24.164950812171128	27.74822695035461	27.024708304735757	21.062113932738505
20	23.19549302219172	27.89693433996797	27.50228780599405	21.40528483184626
21	22.3804621368108	28.583276138183482	27.425074353694807	21.611187371310912
22	22.520590253946466	28.160032029283915	27.110501029512697	22.20887668725692
23	22.33756577442233	27.854037977579498	27.845458705101805	21.962937542896363
24	22.203157172271794	28.646190803019906	26.684397163120565	22.466254861587736
25	23.14687714481812	28.31159917638984	27.107641272020132	21.433882406771907
26	22.663578128574695	28.182910089224432	27.362159688858384	21.791352093342482
27	22.697895218485474	27.965568519789525	27.059025394646536	22.27751086707847
28	22.334706016929765	28.208647906657514	27.69389155799588	21.762754518416838
29	23.55582246625486	28.019903912148248	26.55856783344772	21.865705788149164
30	22.6149622512011	27.968428277282086	27.107641272020132	22.308968199496682
31	22.706474490963167	27.985586822237472	27.18199496682681	22.125943719972547
32	21.871425303134295	28.634751773049643	26.81022649279341	22.68359643102265
33	21.38812628689087	28.351635781285744	27.928391672386184	22.3318462594372
34	22.898078242965	27.816861130176164	27.42793411118737	21.85712651567147
35	22.386181651795926	28.137153969343398	27.46797071608328	22.008693662777397
36	23.490048043925878	28.022763669640817	26.761610615419812	21.7255776710135
37	22.85232212308396	27.648135438114846	27.376458476321208	22.12308396247998
38	22.509151223976207	27.836879432624112	27.219171814230155	22.434797529169526
39	22.083047357584075	28.2343857240906	27.956989247311824	21.7255776710135
40	22.334706016929765	28.34877602379318	27.72248913292153	21.594028826355522
41	22.30038892701899	27.55090368336765	27.5795012582933	22.569206131320062
42	22.266071837108214	28.165751544269046	27.167696179363993	22.40048043925875
43	23.206932052161978	27.199153511782203	27.18771448181194	22.40619995424388
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	3.0
4	5.0
5	5.0
6	6.0
7	7.0
8	11.0
9	15.0
10	16.5
11	18.0
12	18.0
13	41.5
14	65.0
15	88.0
16	111.0
17	135.5
18	160.0
19	160.0
20	179.0
21	198.0
22	231.0
23	264.0
24	387.0
25	510.0
26	510.0
27	669.0
28	828.0
29	973.5
30	1119.0
31	1331.0
32	1543.0
33	1543.0
34	1817.5
35	2092.0
36	2269.5
37	2447.0
38	2611.5
39	2776.0
40	2776.0
41	2922.5
42	3069.0
43	3027.5
44	2986.0
45	2920.5
46	2855.0
47	2855.0
48	2735.0
49	2615.0
50	2540.0
51	2465.0
52	2344.0
53	2223.0
54	2223.0
55	2061.0
56	1899.0
57	1626.5
58	1354.0
59	1249.0
60	1144.0
61	1144.0
62	979.0
63	814.0
64	676.0
65	538.0
66	442.5
67	347.0
68	347.0
69	270.5
70	194.0
71	159.0
72	124.0
73	91.5
74	59.0
75	59.0
76	58.0
77	57.0
78	39.0
79	21.0
80	17.5
81	14.0
82	14.0
83	14.5
84	15.0
85	12.0
86	9.0
87	6.5
88	4.0
89	4.0
90	3.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	34968.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.95950583390528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.48500394632991	82.1636925188744
2	2.999210734017364	5.216197666437886
3	0.9635622204682979	2.51372683596431
4	0.5360431465403841	1.864561885152139
5	0.2762430939226519	1.2010981468771447
6	0.1973164956590371	1.029512697323267
7	0.11510128913443832	0.7006405856783345
8	0.0953696395685346	0.6634637382749943
9	0.06248355695869508	0.48901853122855177
>10	0.2663772691397001	4.000800732097918
>50	0.0032886082609839516	0.15728666209105469
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	55	0.15728666209105469	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	50	0.14298787462823154	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	44	0.12582932967284374	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38	0.10867078471745596	No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG	36	0.10295126973232671	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	36	0.10295126973232671	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35	0.10009151223976206	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.008579272477693892	0.0	0.0
8	0.0	0.0	0.008579272477693892	0.0	0.0
9	0.0	0.0	0.008579272477693892	0.0	0.0
10	0.0	0.0	0.008579272477693892	0.0	0.0
11	0.0	0.0	0.011439029970258523	0.0	0.0
12	0.0	0.0	0.011439029970258523	0.0	0.0
13	0.0	0.0	0.011439029970258523	0.0	0.0
14	0.0	0.0	0.011439029970258523	0.0	0.0
15	0.0	0.0	0.011439029970258523	0.0	0.0
16	0.0	0.0	0.011439029970258523	0.0	0.0
17	0.0	0.0	0.014298787462823153	0.0	0.0
18	0.0	0.0	0.014298787462823153	0.0	0.0
19	0.0	0.0	0.014298787462823153	0.0	0.0
20	0.0	0.0	0.014298787462823153	0.0	0.0
21	0.0	0.0	0.014298787462823153	0.0	0.0
22	0.0	0.0	0.014298787462823153	0.0	0.0
23	0.0	0.0	0.014298787462823153	0.0	0.0
24	0.0	0.0	0.014298787462823153	0.0	0.0
25	0.0	0.0	0.014298787462823153	0.0	0.0
26	0.0	0.0	0.014298787462823153	0.0	0.0
27	0.0	0.0	0.014298787462823153	0.0	0.0
28	0.0	0.0	0.014298787462823153	0.0028597574925646307	0.0
29	0.0	0.0	0.014298787462823153	0.0028597574925646307	0.0
30	0.0	0.0	0.014298787462823153	0.017158544955387784	0.0
31	0.0	0.0	0.014298787462823153	0.025737817433081674	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	80	0.006138992	13.875	4
ATCAACG	80	0.006138992	13.875	3
CAACGCA	85	0.00916834	13.058824	5
>>END_MODULE