FastQCFastQC Report
Fri 10 Feb 2017
ERR1632872.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632872.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440691
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15080.34218987907626885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10000.22691636543519153No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA7910.17949084505923651No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4790.10869293904345677No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA4550.10324694627301216No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGAC200.001840704737.031
GGTATCA3400.025.0294111
TTGCCGA400.00192930823.12510
GCCGTTA502.6984393E-422.215
CCGTTAA502.6984393E-422.216
GCCGCGT603.7195787E-521.58333236
ATGCCGC603.7195787E-521.58333234
TGCCGCG603.7195787E-521.58333235
GTATCAA10000.021.461
AGACTGC555.1361776E-420.181826
GGTACCG555.1361776E-420.181826
TGCCCGC656.889792E-519.92307730
CGTGCTA609.224501E-418.534
ACGTGCT609.224501E-418.533
CGGGATA609.224501E-418.517
CGCAGAA701.2170332E-418.529
TCTCCGA609.224501E-418.511
CCGCGTG701.2170332E-418.537
GTAAGAT752.0636241E-417.2666663
GGCCGTT650.001577844517.07692314