##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632872.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440691
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.782117175072784	33.0	33.0	33.0	33.0	33.0
2	31.295134232375972	33.0	33.0	33.0	27.0	33.0
3	31.73990619277453	33.0	33.0	33.0	27.0	33.0
4	31.859209741065737	33.0	33.0	33.0	33.0	33.0
5	31.975570184097247	33.0	33.0	33.0	33.0	33.0
6	34.90105538801564	37.0	37.0	37.0	27.0	37.0
7	35.07959772266736	37.0	37.0	37.0	33.0	37.0
8	35.25968762693134	37.0	37.0	37.0	33.0	37.0
9	35.32486254541164	37.0	37.0	37.0	33.0	37.0
10	35.358879577754024	37.0	37.0	37.0	33.0	37.0
11	35.37897075274966	37.0	37.0	37.0	33.0	37.0
12	35.3410053756487	37.0	37.0	37.0	33.0	37.0
13	35.377906514995765	37.0	37.0	37.0	33.0	37.0
14	35.34603157314309	37.0	37.0	37.0	33.0	37.0
15	35.36753416793172	37.0	37.0	37.0	33.0	37.0
16	35.36269177269334	37.0	37.0	37.0	33.0	37.0
17	35.34592265328768	37.0	37.0	37.0	33.0	37.0
18	35.34774479170212	37.0	37.0	37.0	33.0	37.0
19	35.35464758753866	37.0	37.0	37.0	33.0	37.0
20	35.35213335420964	37.0	37.0	37.0	33.0	37.0
21	35.34281616824487	37.0	37.0	37.0	33.0	37.0
22	35.35478373735792	37.0	37.0	37.0	33.0	37.0
23	35.34258925187943	37.0	37.0	37.0	33.0	37.0
24	35.33413434810332	37.0	37.0	37.0	33.0	37.0
25	35.324876160393565	37.0	37.0	37.0	33.0	37.0
26	35.305184358201096	37.0	37.0	37.0	33.0	37.0
27	35.24882060219065	37.0	37.0	37.0	33.0	37.0
28	35.21377110038553	37.0	37.0	37.0	33.0	37.0
29	35.256222614031145	37.0	37.0	37.0	33.0	37.0
30	35.22360792482715	37.0	37.0	37.0	33.0	37.0
31	35.21685716295545	37.0	37.0	37.0	33.0	37.0
32	35.24062211390748	37.0	37.0	37.0	33.0	37.0
33	35.23710491024323	37.0	37.0	37.0	33.0	37.0
34	35.17697888089387	37.0	37.0	37.0	33.0	37.0
35	35.164598323995726	37.0	37.0	37.0	33.0	37.0
36	35.15150751887377	37.0	37.0	37.0	33.0	37.0
37	35.18126760020059	37.0	37.0	37.0	33.0	37.0
38	35.03560544690043	37.0	37.0	37.0	27.0	37.0
39	35.10369850984023	37.0	37.0	37.0	33.0	37.0
40	35.1346294796127	37.0	37.0	37.0	33.0	37.0
41	35.153347810597445	37.0	37.0	37.0	33.0	37.0
42	35.077793737562146	37.0	37.0	37.0	33.0	37.0
43	34.87186713592971	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	13.0
16	20.0
17	18.0
18	26.0
19	19.0
20	22.0
21	62.0
22	196.0
23	466.0
24	1046.0
25	1890.0
26	3241.0
27	4938.0
28	6925.0
29	9613.0
30	12566.0
31	16363.0
32	20940.0
33	28232.0
34	39907.0
35	74126.0
36	220060.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.26519488712045	20.609678890651274	13.588886544086446	24.53623967814183
2	15.639302822158838	23.220805507714022	38.468450683131714	22.67144098699542
3	17.925939036649265	26.978086686589926	30.650274228427627	24.445700048333187
4	12.458162295122886	17.888951669083326	40.23635608623729	29.41652994955649
5	13.560068165676176	37.0490888173346	35.9052034191758	13.485639597813433
6	29.024872302815353	40.37409432005646	17.555838444624463	13.045194932503726
7	25.9533323802846	32.290652634158626	24.098971841948213	17.65704314360856
8	24.32339212736362	35.47020474663653	21.42090489708208	18.785498228917767
9	25.79812158632693	15.551940021466287	20.752863117240878	37.89707527496591
10	14.4216696052336	28.690851412894748	34.60224964884692	22.285229333024727
11	33.557980535114176	24.216514519243642	24.49403323417088	17.731471711471304
12	22.882700123215585	25.784279688035383	30.93051593974009	20.402504249008942
13	28.265610144069203	22.3637423954653	26.319575394097	23.0510720663685
14	21.737226310498738	21.318792532636248	28.373849250381788	28.570131906483226
15	23.438645218531807	29.44580216069763	24.627460057046775	22.48809256372379
16	22.38802244656687	28.89121856357402	25.617496159440517	23.103262830418593
17	22.170636568479956	27.80315459131228	26.880739565818228	23.145469274389537
18	22.847755002938566	27.202280055639893	28.398356217848786	21.551608723572752
19	24.409393429863556	26.539003519472825	28.000117996510028	21.05148505415359
20	24.425050659078586	26.652461702190422	27.425338842862683	21.497148795868306
21	23.15341134717977	27.071349312783788	27.58644946232167	22.188789877714772
22	22.3959645193571	27.388124558931317	27.964038294405828	22.251872627305755
23	22.555713640623477	27.052288338087234	27.85716068628585	22.534837335003438
24	22.308147885933682	27.192749568291614	27.723960779775396	22.775141765999305
25	22.86840439219317	27.55536192025705	27.467772203199065	22.108461484350713
26	22.771511104152342	27.40854703182048	27.576918974973395	22.243022889053783
27	22.789664413387158	27.116278753139955	27.517013054498502	22.577043778974385
28	22.588162680880707	27.175277008153103	27.668366270243776	22.56819404072241
29	23.02497668434345	27.399697293568508	27.539250858311153	22.036075163776886
30	23.207417442153346	27.218618033951223	27.40242028995373	22.171544233941695
31	22.79465657342673	27.34251436947884	27.54129310560007	22.321535951494358
32	21.96573109049198	27.195472564676837	27.894828802948098	22.943967541883087
33	22.01792185454207	27.267631968885226	28.144663721292247	22.569782455280457
34	22.90698017431715	26.611616756412086	27.889609726543085	22.59179334272767
35	22.435901799673694	27.22134103033645	27.615494757097377	22.72726241289248
36	22.63422670306405	27.322545729320545	27.726003027064316	22.317224540551088
37	22.884742370504505	26.897304460494997	27.69219248861447	22.525760680386032
38	22.979593411256413	27.16892334992092	27.438273075692493	22.413210163130177
39	22.60450065919204	26.95811804643163	27.837192046127555	22.60018924824877
40	22.362153980907255	27.014166388694115	28.07500039710364	22.548679233294987
41	22.464720178083965	26.50156231917602	28.089976877222362	22.943740625517652
42	22.282733253004942	27.029369785178275	27.964719043502136	22.723177918314647
43	22.60450065919204	26.782257863219357	28.020994302130063	22.592247175458542
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	21.5
2	26.0
3	96.0
4	166.0
5	166.0
6	208.0
7	250.0
8	234.5
9	219.0
10	282.5
11	346.0
12	346.0
13	583.0
14	820.0
15	1381.0
16	1942.0
17	2409.0
18	2876.0
19	2876.0
20	2881.5
21	2887.0
22	3617.0
23	4347.0
24	5911.5
25	7476.0
26	7476.0
27	9542.5
28	11609.0
29	13966.5
30	16324.0
31	18799.5
32	21275.0
33	21275.0
34	23601.0
35	25927.0
36	27717.0
37	29507.0
38	30723.5
39	31940.0
40	31940.0
41	32438.0
42	32936.0
43	32981.5
44	33027.0
45	32458.0
46	31889.0
47	31889.0
48	31190.0
49	30491.0
50	29867.5
51	29244.0
52	28012.5
53	26781.0
54	26781.0
55	25353.0
56	23925.0
57	21756.0
58	19587.0
59	18191.5
60	16796.0
61	16796.0
62	14932.0
63	13068.0
64	11118.0
65	9168.0
66	7593.0
67	6018.0
68	6018.0
69	5110.5
70	4203.0
71	3560.0
72	2917.0
73	2069.5
74	1222.0
75	1222.0
76	919.0
77	616.0
78	480.5
79	345.0
80	261.0
81	177.0
82	177.0
83	153.0
84	129.0
85	111.5
86	94.0
87	78.5
88	63.0
89	63.0
90	49.5
91	36.0
92	20.5
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	440691.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.78294491447753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.20030290453694	68.02809870303591
2	4.588578870993534	6.771177241485028
3	1.055853598848407	2.337119637647546
4	0.5043394217651338	1.4884659109718528
5	0.31144520412145066	1.14896721697856
6	0.20166837380630698	0.8927811909325807
7	0.17186013183985374	0.8876242648374342
8	0.11760452560111959	0.6941766587296535
9	0.07970166522249447	0.5292561217233094
>10	0.6750011993324215	9.6056433032418
>50	0.06397468498538045	3.3085010439313693
>100	0.028742249604873968	3.5559146079990915
>500	6.181128947284724E-4	0.40840342842929156
>1k	3.090564473642362E-4	0.3438706700566006
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1508	0.34218987907626885	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1000	0.22691636543519153	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	791	0.17949084505923651	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	479	0.10869293904345677	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	455	0.10324694627301216	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.2691636543519154E-4	0.0	0.0
2	0.0	0.0	4.538327308703831E-4	0.0	0.0
3	0.0	0.0	4.538327308703831E-4	2.2691636543519154E-4	0.0
4	0.0	0.0	6.807490963055747E-4	2.2691636543519154E-4	0.0
5	0.0	0.0	9.076654617407662E-4	2.2691636543519154E-4	0.0
6	0.0	0.0	0.001588414558046341	2.2691636543519154E-4	0.0
7	0.0	0.0	0.0036306618469630647	2.2691636543519154E-4	0.0
8	0.0	0.0	0.005219076405009406	2.2691636543519154E-4	0.0
9	0.0	0.0	0.005672909135879789	0.0013614981926111493	0.0
10	2.2691636543519154E-4	0.0	0.00589982550131498	0.0018153309234815323	0.0
11	2.2691636543519154E-4	0.0	0.010438152810018812	0.0018153309234815323	0.0
12	2.2691636543519154E-4	0.0	0.011118901906324386	0.0018153309234815323	0.0
13	2.2691636543519154E-4	0.0	0.012253483733500345	0.0018153309234815323	0.0
14	2.2691636543519154E-4	0.0	0.013388065560676302	0.002042247288916724	0.0
15	2.2691636543519154E-4	0.0	0.015203396484157835	0.002042247288916724	0.0
16	4.538327308703831E-4	0.0	0.016564894676768985	0.002042247288916724	0.0
17	4.538327308703831E-4	0.0	0.016791811042204174	0.0022691636543519154	0.0
18	4.538327308703831E-4	0.0	0.01747256013850975	0.0022691636543519154	0.0
19	4.538327308703831E-4	0.0	0.01747256013850975	0.0022691636543519154	0.0
20	4.538327308703831E-4	0.0	0.017926392869380133	0.0022691636543519154	0.0
21	4.538327308703831E-4	0.0	0.0188340583311209	0.0022691636543519154	0.0
22	4.538327308703831E-4	0.0	0.0188340583311209	0.002496080019787107	0.0
23	4.538327308703831E-4	0.0	0.0188340583311209	0.00294991275065749	0.0
24	4.538327308703831E-4	0.0	0.0188340583311209	0.00294991275065749	0.0
25	4.538327308703831E-4	0.0	0.0188340583311209	0.00294991275065749	0.0
26	4.538327308703831E-4	0.0	0.0188340583311209	0.004084494577833448	0.0
27	4.538327308703831E-4	0.0	0.0188340583311209	0.004765243674139023	0.0
28	4.538327308703831E-4	0.0	0.0188340583311209	0.011118901906324386	0.0
29	4.538327308703831E-4	0.0	0.0188340583311209	0.024053134736130303	0.0
30	4.538327308703831E-4	0.0	0.0188340583311209	0.0428871930672512	0.0
31	4.538327308703831E-4	0.0	0.0188340583311209	0.11345818271759578	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGCGAC	20	0.0018407047	37.0	31
GGTATCA	340	0.0	25.029411	1
TTGCCGA	40	0.001929308	23.125	10
GCCGTTA	50	2.6984393E-4	22.2	15
CCGTTAA	50	2.6984393E-4	22.2	16
GCCGCGT	60	3.7195787E-5	21.583332	36
ATGCCGC	60	3.7195787E-5	21.583332	34
TGCCGCG	60	3.7195787E-5	21.583332	35
GTATCAA	1000	0.0	21.46	1
AGACTGC	55	5.1361776E-4	20.18182	6
GGTACCG	55	5.1361776E-4	20.18182	6
TGCCCGC	65	6.889792E-5	19.923077	30
CGTGCTA	60	9.224501E-4	18.5	34
ACGTGCT	60	9.224501E-4	18.5	33
CGGGATA	60	9.224501E-4	18.5	17
CGCAGAA	70	1.2170332E-4	18.5	29
TCTCCGA	60	9.224501E-4	18.5	11
CCGCGTG	70	1.2170332E-4	18.5	37
GTAAGAT	75	2.0636241E-4	17.266666	3
GGCCGTT	65	0.0015778445	17.076923	14
>>END_MODULE