FastQCFastQC Report
Fri 10 Feb 2017
ERR1632871.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632871.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415354
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10980.2643528171150392No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7510.18080962263514977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4210.10135932240931832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGTA250.00549235429.5999989
TAACGGC706.5665517E-926.4285736
TTAACGG659.361611E-825.61538535
GTATCAA7500.024.1733321
TAAGACA400.001929122823.1254
GGTATCA2750.022.21
GTATTGG502.6980788E-422.1999991
TAGTACC705.0889885E-621.1428574
AACGGCC806.9381167E-720.812537
TATCGCG806.9381167E-720.81257
TCTATAC656.888576E-519.9230773
TTTAACG851.2413948E-619.58823434
ATAATAC701.21682024E-418.53
CTTGCGG609.223285E-418.534
GTATCGC902.145489E-618.56
ATGCCCG801.6126236E-518.529
ATCGCGC902.145489E-618.58
TACCGCA701.21682024E-418.57
TCTATAG701.21682024E-418.51
TGCGGTA500.00702750618.49999836
Produced by FastQC (version 0.11.5)