##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632871.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	415354
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.714641005022223	33.0	33.0	33.0	27.0	33.0
2	31.237130255155844	33.0	33.0	33.0	27.0	33.0
3	31.691949517760754	33.0	33.0	33.0	27.0	33.0
4	31.82069030272972	33.0	33.0	33.0	27.0	33.0
5	31.93594861250885	33.0	33.0	33.0	33.0	33.0
6	34.84803324393168	37.0	37.0	37.0	27.0	37.0
7	35.00642102880916	37.0	37.0	37.0	33.0	37.0
8	35.18055441864048	37.0	37.0	37.0	33.0	37.0
9	35.26270843665885	37.0	37.0	37.0	33.0	37.0
10	35.30669260438084	37.0	37.0	37.0	33.0	37.0
11	35.314948212849764	37.0	37.0	37.0	33.0	37.0
12	35.27086051897899	37.0	37.0	37.0	33.0	37.0
13	35.30035343345676	37.0	37.0	37.0	33.0	37.0
14	35.28064012866133	37.0	37.0	37.0	33.0	37.0
15	35.305418991992376	37.0	37.0	37.0	33.0	37.0
16	35.28790621975472	37.0	37.0	37.0	33.0	37.0
17	35.25519436432537	37.0	37.0	37.0	33.0	37.0
18	35.274536901053075	37.0	37.0	37.0	33.0	37.0
19	35.27701671345407	37.0	37.0	37.0	33.0	37.0
20	35.288551452495945	37.0	37.0	37.0	33.0	37.0
21	35.26784863032498	37.0	37.0	37.0	33.0	37.0
22	35.27000101118564	37.0	37.0	37.0	33.0	37.0
23	35.26348849415198	37.0	37.0	37.0	33.0	37.0
24	35.24977007564632	37.0	37.0	37.0	33.0	37.0
25	35.266538904163674	37.0	37.0	37.0	33.0	37.0
26	35.21590017190156	37.0	37.0	37.0	33.0	37.0
27	35.17164635467577	37.0	37.0	37.0	33.0	37.0
28	35.15085685945001	37.0	37.0	37.0	33.0	37.0
29	35.177044159921415	37.0	37.0	37.0	33.0	37.0
30	35.14384837993615	37.0	37.0	37.0	33.0	37.0
31	35.15110484069011	37.0	37.0	37.0	33.0	37.0
32	35.19704396731463	37.0	37.0	37.0	33.0	37.0
33	35.17496641419127	37.0	37.0	37.0	33.0	37.0
34	35.1147262335261	37.0	37.0	37.0	33.0	37.0
35	35.102500517630745	37.0	37.0	37.0	33.0	37.0
36	35.08657915898246	37.0	37.0	37.0	33.0	37.0
37	35.113941360863265	37.0	37.0	37.0	33.0	37.0
38	34.95896512372578	37.0	37.0	37.0	27.0	37.0
39	35.03571892891365	37.0	37.0	37.0	27.0	37.0
40	35.09020498177458	37.0	37.0	37.0	33.0	37.0
41	35.096671754696	37.0	37.0	37.0	33.0	37.0
42	35.017955767851035	37.0	37.0	37.0	27.0	37.0
43	34.80878238803527	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	15.0
16	19.0
17	25.0
18	15.0
19	18.0
20	28.0
21	83.0
22	204.0
23	541.0
24	1067.0
25	1939.0
26	3219.0
27	4939.0
28	6936.0
29	9652.0
30	12453.0
31	15877.0
32	20295.0
33	26750.0
34	38567.0
35	70294.0
36	202413.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.77076421558478	20.144744001502332	13.54844301487406	25.536048768038828
2	16.255531426205117	22.5166966009717	37.72541013208011	23.502361840743077
3	18.13416988881773	26.002879471486974	29.79747396196979	26.065476677725506
4	13.057295704387101	17.82792509521998	38.50281928186559	30.611959918527326
5	14.756809853763295	36.0896969813701	34.90131309678009	14.252180068086501
6	30.422964507384066	38.87816176081126	17.285255468829	13.41361826297568
7	27.28901130120331	31.591365437674853	23.072367185581456	18.047256075540382
8	24.75647279188355	35.63899709645266	20.33638775598646	19.268142355677327
9	26.20054218810942	15.391449221627818	19.869557052538315	38.53845153772445
10	15.33535249449867	27.605608709678975	33.507321465545054	23.551717330277306
11	34.63455269480973	23.228137925721192	23.870000048151695	18.26730933131738
12	23.391372178912444	25.098590599825695	30.031009692936628	21.479027528325236
13	28.307660453492684	21.672597350693625	25.652094358065654	24.36764783774804
14	22.30771823552921	20.489028635814268	27.19680080124424	30.00645232741228
15	24.22415578037048	28.202689753800374	23.9015394097565	23.67161505607265
16	23.574589386402923	27.783288472002194	24.789456704401548	23.852665437193334
17	23.01458514905358	27.077866109391024	26.06668047015317	23.840868271402226
18	23.686542082175684	26.717209898062855	27.021528623776348	22.574719395985113
19	24.888408441955537	26.134815121558958	26.487767061350077	22.489009375135428
20	24.687134348050098	25.88225947023503	26.63270366964084	22.79790251207404
21	23.665836852419865	26.63487049601063	26.563365225807384	23.13592742576212
22	23.38824231860052	26.521232490839143	26.563365225807384	23.527159964752958
23	23.42580064234364	26.243878715505325	26.970728583328917	23.359592058822113
24	23.618407430769896	26.67315109521035	26.022862425786197	23.685579048233553
25	23.80090236280378	26.35534989430702	26.676762472493344	23.166985270395855
26	23.758769627835534	26.598275206209642	26.480544306784093	23.16241085917073
27	23.658132580882814	26.641370975120015	26.22509955363377	23.475396890363402
28	23.532215892949147	26.34138590214612	26.7461009163268	23.380297288577935
29	24.17961546054691	26.392667459564613	26.225581070604832	23.20213600928365
30	23.773455895453036	26.287696759872304	26.542659996051558	23.3961873486231
31	23.78814216307054	25.969895558968975	26.58431121404874	23.657651063911747
32	23.004232534175667	26.210894802987333	26.89922331312567	23.88564934971133
33	23.15470658763368	26.19067109020257	27.15057517202194	23.50404715014181
34	23.726507990774135	25.817495437626697	26.798586266172954	23.657410305426215
35	23.469859445196146	26.52291780023787	26.53832634331197	23.468896411254015
36	24.08499737573251	26.16394689830843	26.407353727182116	23.343701998776947
37	23.741194258391637	25.95280170649615	26.338496800319728	23.96750723479249
38	23.70724731193151	26.32766266847075	26.336089215464398	23.62900080413334
39	23.3646479870183	26.121573404854654	26.75308291240725	23.760695695719797
40	23.247157846078284	26.593941553470053	26.78052937975799	23.378371220693673
41	23.501158048315414	25.908502145158106	26.813513292275985	23.776826514250494
42	23.387760801629454	26.552290335472872	26.641130216634483	23.418818646263187
43	23.61335150257371	25.70169060608541	26.796419439803152	23.888538451537723
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	9.5
2	15.0
3	61.0
4	107.0
5	107.0
6	138.5
7	170.0
8	160.5
9	151.0
10	201.5
11	252.0
12	252.0
13	406.0
14	560.0
15	995.5
16	1431.0
17	1663.5
18	1896.0
19	1896.0
20	1851.0
21	1806.0
22	2090.0
23	2374.0
24	3306.0
25	4238.0
26	4238.0
27	5777.0
28	7316.0
29	8962.5
30	10609.0
31	12971.5
32	15334.0
33	15334.0
34	17790.0
35	20246.0
36	22402.0
37	24558.0
38	26499.5
39	28441.0
40	28441.0
41	29860.5
42	31280.0
43	32281.5
44	33283.0
45	32832.5
46	32382.0
47	32382.0
48	32026.0
49	31670.0
50	31335.5
51	31001.0
52	29869.5
53	28738.0
54	28738.0
55	27364.0
56	25990.0
57	23600.5
58	21211.0
59	19605.0
60	17999.0
61	17999.0
62	15988.0
63	13977.0
64	11799.0
65	9621.0
66	8290.5
67	6960.0
68	6960.0
69	5776.5
70	4593.0
71	3803.5
72	3014.0
73	2433.5
74	1853.0
75	1853.0
76	1403.5
77	954.0
78	747.0
79	540.0
80	429.5
81	319.0
82	319.0
83	252.0
84	185.0
85	168.5
86	152.0
87	114.5
88	77.0
89	77.0
90	59.0
91	41.0
92	23.0
93	5.0
94	3.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	415354.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.2050607334744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.89587794359728	63.2983638792937
2	6.538868093047337	9.312009993872271
3	1.7962208846603558	3.837000515489273
4	0.7848733733307377	2.235478248644084
5	0.44274200264061375	1.5762735593642492
6	0.28174965512629796	1.2037200782942106
7	0.20251003153941002	1.0093817366431053
8	0.1488003884042456	0.8476272554791109
9	0.11229134197115814	0.7196140642409692
>10	0.7084928320727926	9.461412486163404
>50	0.059061166419390736	2.816929061822492
>100	0.027833423209709537	3.235299216908344
>500	3.394319903623114E-4	0.1815112589196299
>1k	3.394319903623114E-4	0.2653786448651846
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1098	0.2643528171150392	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	751	0.18080962263514977	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	421	0.10135932240931832	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	2.407584855328226E-4	2.407584855328226E-4	0.0
4	0.0	0.0	2.407584855328226E-4	2.407584855328226E-4	0.0
5	0.0	0.0	7.222754565984678E-4	2.407584855328226E-4	0.0
6	0.0	0.0	9.630339421312904E-4	2.407584855328226E-4	0.0
7	0.0	0.0	0.0033706187974595165	2.407584855328226E-4	0.0
8	0.0	0.0	0.0038521357685251615	2.407584855328226E-4	0.0
9	0.0	0.0	0.004574411225123629	4.815169710656452E-4	0.0
10	4.815169710656452E-4	0.0	0.004815169710656452	4.815169710656452E-4	0.0
11	4.815169710656452E-4	0.0	0.007704271537050323	4.815169710656452E-4	0.0
12	4.815169710656452E-4	0.0	0.008908063964714436	4.815169710656452E-4	0.0
13	4.815169710656452E-4	0.0	0.009871097906845728	4.815169710656452E-4	0.0
14	4.815169710656452E-4	0.0	0.011315648820042662	4.815169710656452E-4	0.0
15	4.815169710656452E-4	0.0	0.012760199733239597	4.815169710656452E-4	0.0
16	4.815169710656452E-4	0.0	0.013723233675370889	4.815169710656452E-4	0.0
17	4.815169710656452E-4	0.0	0.013723233675370889	4.815169710656452E-4	0.0
18	4.815169710656452E-4	0.0	0.01396399216090371	4.815169710656452E-4	0.0
19	4.815169710656452E-4	0.0	0.01396399216090371	4.815169710656452E-4	0.0
20	4.815169710656452E-4	0.0	0.014445509131969356	4.815169710656452E-4	0.0
21	4.815169710656452E-4	0.0	0.014686267617502179	4.815169710656452E-4	0.0
22	4.815169710656452E-4	0.0	0.014927026103035002	7.222754565984678E-4	0.0
23	4.815169710656452E-4	0.0	0.015167784588567825	0.001203792427664113	0.0
24	4.815169710656452E-4	0.0	0.015890060045166292	0.001203792427664113	0.0
25	4.815169710656452E-4	0.0	0.015890060045166292	0.0016853093987297583	0.0
26	4.815169710656452E-4	0.0	0.015890060045166292	0.0021668263697954032	0.0
27	4.815169710656452E-4	0.0	0.015890060045166292	0.0026483433408610486	0.0
28	4.815169710656452E-4	0.0	0.015890060045166292	0.007222754565984678	0.0
29	4.815169710656452E-4	0.0	0.016130818530699115	0.019742195813691455	0.0
30	4.815169710656452E-4	0.0	0.016130818530699115	0.03972515011291573	0.0
31	4.815169710656452E-4	0.0	0.016130818530699115	0.10039628846718703	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGGTA	25	0.005492354	29.599998	9
TAACGGC	70	6.5665517E-9	26.42857	36
TTAACGG	65	9.361611E-8	25.615385	35
GTATCAA	750	0.0	24.173332	1
TAAGACA	40	0.0019291228	23.125	4
GGTATCA	275	0.0	22.2	1
GTATTGG	50	2.6980788E-4	22.199999	1
TAGTACC	70	5.0889885E-6	21.142857	4
AACGGCC	80	6.9381167E-7	20.8125	37
TATCGCG	80	6.9381167E-7	20.8125	7
TCTATAC	65	6.888576E-5	19.923077	3
TTTAACG	85	1.2413948E-6	19.588234	34
ATAATAC	70	1.21682024E-4	18.5	3
CTTGCGG	60	9.223285E-4	18.5	34
GTATCGC	90	2.145489E-6	18.5	6
ATGCCCG	80	1.6126236E-5	18.5	29
ATCGCGC	90	2.145489E-6	18.5	8
TACCGCA	70	1.21682024E-4	18.5	7
TCTATAG	70	1.21682024E-4	18.5	1
TGCGGTA	50	0.007027506	18.499998	36
>>END_MODULE