FastQCFastQC Report
Fri 10 Feb 2017
ERR1632870.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632870.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562594
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15450.2746207744839085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12210.21703039847563252No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT8380.14895288609547916No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC7430.13206681905601553No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG7330.13028933831501935No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC6710.1192689577208431No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6630.11784697312804616No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA6470.11500300394245229No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA6190.11002605786766301No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC5880.10451586757057488No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC5800.10309388297777794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7550.026.4635731
ATAGCGG358.8637427E-426.4285727
CGCAATC358.8637427E-426.42857212
CGTTTAG851.9717845E-923.94117726
GGTATCA2350.023.6170221
GGTCTAG400.001929967823.1250021
TACGTTA400.001929967823.12500219
GCGTTTA903.8180588E-922.6111125
CGCTAAG450.00382341720.55555535
TTTGCCG450.00382341720.55555527
AATAGCG450.00382341720.5555556
GACTATT450.00382341720.5555557
TGCGTTA1001.2862074E-820.3537
CACGAGG656.894123E-519.92307914
ATGCGTT951.6723061E-719.47368436
GTATTAG1153.0413503E-919.3043481
GCGCAAT500.007030526618.511
CGAGGGT609.2288275E-418.516
TATTAGC801.61428E-518.52
ACTAAGT500.007030526618.58