FastQCFastQC Report
Fri 10 Feb 2017
ERR1632869.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632869.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464159
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12900.2779220051749508No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9500.2046712441210878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5230.11267690597403043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAG303.5962008E-430.83333429
TATCCCG303.5962008E-430.83333410
ACCGATC250.00549300929.627
GTGCGCA250.00549300929.611
GGTATCA2100.028.1904751
GTATCAA7150.025.3566441
ATTTCGG551.8994919E-523.54545429
CGCAATC400.001929462423.12512
ACTAATG652.6771286E-622.7692313
TAATACT1201.0913936E-1121.5833344
TCGACTT450.003822424220.55555513
AGTACTC450.003822424220.5555555
TTGTACT1052.2508175E-819.380954
AATTTCG500.00702871818.528
GTATTAG1205.149559E-918.51
CCCTAGA500.00702871818.51
CGGTACT500.00702871818.51
CTAGGTG500.00702871818.524
CTAGGAC801.6132892E-518.53
GTAGTAC609.2255074E-418.53
Produced by FastQC (version 0.11.5)