##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632869.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	464159
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.728024233075303	33.0	33.0	33.0	27.0	33.0
2	31.252303628713438	33.0	33.0	33.0	27.0	33.0
3	31.701569936164116	33.0	33.0	33.0	27.0	33.0
4	31.823049860069503	33.0	33.0	33.0	27.0	33.0
5	31.941425244366695	33.0	33.0	33.0	33.0	33.0
6	34.81701959888745	37.0	37.0	37.0	27.0	37.0
7	34.97284766642465	37.0	37.0	37.0	27.0	37.0
8	35.17124950717319	37.0	37.0	37.0	33.0	37.0
9	35.25207741312783	37.0	37.0	37.0	33.0	37.0
10	35.29133766661855	37.0	37.0	37.0	33.0	37.0
11	35.32152128904104	37.0	37.0	37.0	33.0	37.0
12	35.276023948689996	37.0	37.0	37.0	33.0	37.0
13	35.29099942045721	37.0	37.0	37.0	33.0	37.0
14	35.28316158902445	37.0	37.0	37.0	33.0	37.0
15	35.2911092965988	37.0	37.0	37.0	33.0	37.0
16	35.28559609961242	37.0	37.0	37.0	33.0	37.0
17	35.25673314532305	37.0	37.0	37.0	33.0	37.0
18	35.266130787079426	37.0	37.0	37.0	33.0	37.0
19	35.26278925971488	37.0	37.0	37.0	33.0	37.0
20	35.26252641874875	37.0	37.0	37.0	33.0	37.0
21	35.27539054505029	37.0	37.0	37.0	33.0	37.0
22	35.2751384762549	37.0	37.0	37.0	33.0	37.0
23	35.26155692338186	37.0	37.0	37.0	33.0	37.0
24	35.26715198886588	37.0	37.0	37.0	33.0	37.0
25	35.25570117136585	37.0	37.0	37.0	33.0	37.0
26	35.21541756165452	37.0	37.0	37.0	33.0	37.0
27	35.16901105009275	37.0	37.0	37.0	33.0	37.0
28	35.135304927837225	37.0	37.0	37.0	33.0	37.0
29	35.18218756934585	37.0	37.0	37.0	33.0	37.0
30	35.13642738802867	37.0	37.0	37.0	33.0	37.0
31	35.130552246105324	37.0	37.0	37.0	33.0	37.0
32	35.16501457474702	37.0	37.0	37.0	33.0	37.0
33	35.147068138288816	37.0	37.0	37.0	33.0	37.0
34	35.07959341518747	37.0	37.0	37.0	33.0	37.0
35	35.07043276118744	37.0	37.0	37.0	33.0	37.0
36	35.064098724790426	37.0	37.0	37.0	27.0	37.0
37	35.10387388804268	37.0	37.0	37.0	33.0	37.0
38	34.94940095958497	37.0	37.0	37.0	27.0	37.0
39	35.01687352825217	37.0	37.0	37.0	27.0	37.0
40	35.052180395080136	37.0	37.0	37.0	27.0	37.0
41	35.061582345704814	37.0	37.0	37.0	33.0	37.0
42	34.98817646539225	37.0	37.0	37.0	27.0	37.0
43	34.77973496151103	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	14.0
16	21.0
17	26.0
18	21.0
19	17.0
20	36.0
21	86.0
22	261.0
23	569.0
24	1200.0
25	2235.0
26	3792.0
27	5631.0
28	7802.0
29	10714.0
30	13997.0
31	17984.0
32	22817.0
33	29972.0
34	42894.0
35	78309.0
36	225759.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.5109908458093	19.877886672454913	13.272607016130248	25.33851546560554
2	16.29140014520886	23.055246154873654	37.534767181073725	23.11858651884376
3	18.202383235055226	26.169049829907426	30.298238319196653	25.330328615840692
4	13.127398154511708	17.708586928186246	38.44156851423758	30.722446403064467
5	14.166481744402242	36.83500696959447	35.16769038195963	13.830820904043659
6	29.99209322667448	39.012493563627984	17.441867980584238	13.5535452291133
7	26.814733744255744	31.784797881760348	23.316579017104054	18.08388935687986
8	24.77642359622457	35.41480397880898	20.73944488849726	19.06932753646918
9	26.338388353990766	15.38955401058689	19.77145762551195	38.50060000991039
10	15.025454639466219	28.002688733817504	33.774417818032184	23.197438808684094
11	34.191947156039205	23.364838342033657	24.001042746127943	18.442171755799198
12	22.925118332295614	25.20278611424103	30.320859877757407	21.551235675705954
13	28.613901701787537	21.94851333271573	26.116481636680533	23.321103328816204
14	22.362380132670054	20.82195971639029	27.525481569893074	29.290178581046582
15	24.23716872881922	28.217916705266944	23.941364920210532	23.603549645703303
16	23.488071975336037	27.940425586921723	24.81240264650691	23.75909979123533
17	22.819551059012106	27.413235550748773	26.13091634547644	23.636297044762678
18	23.553566773454786	26.796421053992276	27.191328833438543	22.458683339114398
19	25.09527985022374	26.05637292393339	26.935812943409477	21.91253428243339
20	24.93563627980929	25.821755045146165	26.935382056579748	22.307226618464792
21	23.570371359814203	26.702056838281706	26.42973636189323	23.297835440010857
22	23.162752418890943	26.72855637831002	26.517206388328134	23.591484814470903
23	23.061278570489854	26.60596907525223	27.145439386072447	23.187312968185473
24	23.448861273830733	26.739759435882966	26.349806854978574	23.46157243530773
25	23.600533437895205	26.48984507464037	26.948524104886474	22.961097382577954
26	23.581143530557416	26.55275455178075	26.78909597788689	23.077005939774946
27	23.813606975196	26.5264704551673	26.24036160022751	23.41956096940919
28	23.20023957307733	26.55706342007803	27.054953151829437	23.1877438550152
29	23.767286641000172	26.65465928701156	26.36790410182718	23.210149970161087
30	23.56778603883583	26.735666011000543	26.739113105638367	22.95743484452526
31	23.889658500643097	26.356701044254233	26.714337112928977	23.039303342173696
32	22.740914212586635	26.467008072664754	26.893155147266345	23.898922567482263
33	22.981779950404928	26.44761816532697	27.238295497878962	23.332306386389146
34	23.2058411018638	26.18456175577765	27.007340157144426	23.60225698521412
35	23.460495218233408	26.705288489504674	26.873980683343422	22.960235608918495
36	23.672276095045017	26.539612503474025	26.659829928968303	23.12828147251265
37	23.789908199560927	26.089982096652225	26.532071983953777	23.588037719833075
38	23.926930211414625	26.333433155448887	26.578392318149604	23.161244314986888
39	23.42149996014297	26.20804508799786	26.930642301452735	23.439812650406434
40	23.164475966209856	26.573221676192855	27.050428840117284	23.211873517480004
41	23.16512229645445	25.905347090113516	27.32188754284631	23.607643070585727
42	23.436365555768603	26.188655180660074	26.834985425252984	23.539993838318335
43	23.287278712682507	25.875831342277106	27.162890302676452	23.67399964236393
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	17.5
2	24.0
3	89.0
4	154.0
5	154.0
6	185.0
7	216.0
8	198.5
9	181.0
10	250.0
11	319.0
12	319.0
13	480.0
14	641.0
15	1244.0
16	1847.0
17	2132.0
18	2417.0
19	2417.0
20	2336.0
21	2255.0
22	2716.5
23	3178.0
24	4216.0
25	5254.0
26	5254.0
27	6970.5
28	8687.0
29	10952.5
30	13218.0
31	15499.5
32	17781.0
33	17781.0
34	20103.0
35	22425.0
36	24657.0
37	26889.0
38	29371.5
39	31854.0
40	31854.0
41	33421.5
42	34989.0
43	34962.0
44	34935.0
45	35576.5
46	36218.0
47	36218.0
48	35705.0
49	35192.0
50	35476.0
51	35760.0
52	35336.5
53	34913.0
54	34913.0
55	32026.0
56	29139.0
57	26431.0
58	23723.0
59	21188.0
60	18653.0
61	18653.0
62	16648.0
63	14643.0
64	12112.0
65	9581.0
66	8242.5
67	6904.0
68	6904.0
69	5789.0
70	4674.0
71	3880.0
72	3086.0
73	2509.5
74	1933.0
75	1933.0
76	1493.5
77	1054.0
78	834.5
79	615.0
80	474.5
81	334.0
82	334.0
83	254.0
84	174.0
85	164.5
86	155.0
87	118.5
88	82.0
89	82.0
90	61.0
91	40.0
92	25.0
93	10.0
94	5.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	464159.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.8167408642769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.35413243319493	56.62010165346122
2	6.955385178051622	9.01650797394009
3	2.0102527347841006	3.9089409174661505
4	0.9303871153966143	2.412186422484978
5	0.5612300487458887	1.8188551317403878
6	0.3768650825051898	1.4656299836119933
7	0.27192375354189535	1.233764802771657
8	0.21074851802567615	1.0928025664320513
9	0.1583209549435735	0.9235663478966217
>10	1.045660766551919	13.367194282129732
>50	0.08562543874097828	3.8658953144207926
>100	0.03846455241124125	3.6755489552257714
>500	6.689487375868043E-4	0.31933960192565536
>1k	3.3447436879340214E-4	0.27966604649293647
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1290	0.2779220051749508	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	950	0.2046712441210878	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	523	0.11267690597403043	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	4.308868297286059E-4	0.0	0.0
4	0.0	0.0	4.308868297286059E-4	0.0	0.0
5	0.0	0.0	4.308868297286059E-4	0.0	0.0
6	0.0	0.0	8.617736594572119E-4	2.1544341486430296E-4	0.0
7	0.0	0.0	0.0025853209783716358	2.1544341486430296E-4	0.0
8	0.0	0.0	0.0038779814675574532	2.1544341486430296E-4	0.0
9	0.0	0.0	0.004739755127014665	2.1544341486430296E-4	0.0
10	4.308868297286059E-4	0.0	0.005386085371607574	2.1544341486430296E-4	0.0
11	4.308868297286059E-4	0.0	0.00947951025402933	4.308868297286059E-4	0.0
12	4.308868297286059E-4	0.0	0.010772170743215148	6.463302445929089E-4	0.0
13	4.308868297286059E-4	0.0	0.011418500987808057	6.463302445929089E-4	0.0
14	4.308868297286059E-4	0.0	0.011849387817536663	6.463302445929089E-4	0.0
15	4.308868297286059E-4	0.0	0.012495718062129572	8.617736594572119E-4	0.0
16	4.308868297286059E-4	0.0	0.014434708795908299	8.617736594572119E-4	0.0
17	4.308868297286059E-4	0.0	0.014650152210772602	8.617736594572119E-4	0.0
18	4.308868297286059E-4	0.0	0.015081039040501207	8.617736594572119E-4	0.0
19	4.308868297286059E-4	0.0	0.015511925870229813	8.617736594572119E-4	0.0
20	4.308868297286059E-4	0.0	0.01594281269995842	8.617736594572119E-4	0.0
21	4.308868297286059E-4	0.0	0.017235473189144236	0.0012926604891858179	0.0
22	4.308868297286059E-4	0.0	0.01745091660400854	0.0015081039040501208	0.0
23	4.308868297286059E-4	0.0	0.01745091660400854	0.0019389907337787266	0.0
24	4.308868297286059E-4	0.0	0.01745091660400854	0.0021544341486430295	0.0
25	4.308868297286059E-4	0.0	0.01745091660400854	0.0021544341486430295	0.0
26	4.308868297286059E-4	0.0	0.017666360018872843	0.0025853209783716358	0.0
27	4.308868297286059E-4	0.0	0.017666360018872843	0.0030162078081002416	0.0
28	4.308868297286059E-4	0.0	0.017666360018872843	0.010987614158079452	0.0
29	4.308868297286059E-4	0.0	0.017666360018872843	0.0290848610066809	0.0
30	4.308868297286059E-4	0.0	0.017666360018872843	0.05213730639716132	0.0
31	4.308868297286059E-4	0.0	0.017666360018872843	0.12624984111048154	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCAG	30	3.5962008E-4	30.833334	29
TATCCCG	30	3.5962008E-4	30.833334	10
ACCGATC	25	0.005493009	29.6	27
GTGCGCA	25	0.005493009	29.6	11
GGTATCA	210	0.0	28.190475	1
GTATCAA	715	0.0	25.356644	1
ATTTCGG	55	1.8994919E-5	23.545454	29
CGCAATC	40	0.0019294624	23.125	12
ACTAATG	65	2.6771286E-6	22.76923	13
TAATACT	120	1.0913936E-11	21.583334	4
TCGACTT	45	0.0038224242	20.555555	13
AGTACTC	45	0.0038224242	20.555555	5
TTGTACT	105	2.2508175E-8	19.38095	4
AATTTCG	50	0.007028718	18.5	28
GTATTAG	120	5.149559E-9	18.5	1
CCCTAGA	50	0.007028718	18.5	1
CGGTACT	50	0.007028718	18.5	1
CTAGGTG	50	0.007028718	18.5	24
CTAGGAC	80	1.6132892E-5	18.5	3
GTAGTAC	60	9.2255074E-4	18.5	3
>>END_MODULE