FastQCFastQC Report
Fri 10 Feb 2017
ERR1632868.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632868.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453333
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46711.0303684046826505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33940.748677021086045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17200.3794120436853263No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.19500014338245836No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8620.19014719863764606No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8180.18044130914802145No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8110.17889719036558116No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG7450.16433835613114423No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6870.15154422907663903No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6730.14845599151175848No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC6140.1354412760597618No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6140.1354412760597618No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5880.12970597772498363No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5850.12904421253250922No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5790.1277206821475604No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA5530.1219853838127822No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC5430.11977949983786754No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5430.11977949983786754No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA5420.11955891144037605No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5320.11735302746546138No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG5240.11558832028552962No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5210.1149265550930552No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5160.11382361310559787No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5030.11095596393820878No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4970.10963243355325997No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT4900.10808831477081969No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4830.1065441959883794No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG4610.1016912512435671No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT250.005492876729.5999984
GATTATC250.005492876729.5999982
TCGCAGC405.932316E-527.7537
AGCGTCA358.861075E-426.42857237
CCCACGC358.861075E-426.42857219
GCCGCTC1550.026.25806427
GGTATCA7450.025.8255021
CCGCTCT1600.025.437528
CGCTCTC1600.025.437529
CTTAGGA451.3214858E-424.6666662
CGCCGCC1250.023.6833
GCTCTCT1800.023.6388930
GCAGCTT1800.023.6388919
CCGGCAG1650.023.54545416
TTATACC551.8993655E-523.5454544
GTATCAA21000.023.5214271
CTTCGCC1900.023.36842223
GCCGGCA1750.023.25714115
TTAAGCA400.001929392923.12537
CCCTCTC652.6768976E-622.7692332
Produced by FastQC (version 0.11.5)