##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632868.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	453333
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.669185786166018	33.0	33.0	33.0	27.0	33.0
2	31.18380307632579	33.0	33.0	33.0	27.0	33.0
3	31.63173869980787	33.0	33.0	33.0	27.0	33.0
4	31.762688796094704	33.0	33.0	33.0	27.0	33.0
5	31.902497722424798	33.0	33.0	33.0	33.0	33.0
6	34.723446120180974	37.0	37.0	37.0	27.0	37.0
7	34.918088910359494	37.0	37.0	37.0	27.0	37.0
8	35.07956844085915	37.0	37.0	37.0	33.0	37.0
9	35.14871407993682	37.0	37.0	37.0	33.0	37.0
10	35.18410528243035	37.0	37.0	37.0	33.0	37.0
11	35.22445090033155	37.0	37.0	37.0	33.0	37.0
12	35.15063761076295	37.0	37.0	37.0	33.0	37.0
13	35.203514855525626	37.0	37.0	37.0	33.0	37.0
14	35.166868505050374	37.0	37.0	37.0	33.0	37.0
15	35.19671632111494	37.0	37.0	37.0	33.0	37.0
16	35.19366117180969	37.0	37.0	37.0	33.0	37.0
17	35.156765556445265	37.0	37.0	37.0	33.0	37.0
18	35.170978066895636	37.0	37.0	37.0	33.0	37.0
19	35.17136409659125	37.0	37.0	37.0	33.0	37.0
20	35.161536883483	37.0	37.0	37.0	33.0	37.0
21	35.149269962698504	37.0	37.0	37.0	33.0	37.0
22	35.13072950788934	37.0	37.0	37.0	33.0	37.0
23	35.14564128355977	37.0	37.0	37.0	33.0	37.0
24	35.14603172502333	37.0	37.0	37.0	33.0	37.0
25	35.1356927468329	37.0	37.0	37.0	33.0	37.0
26	35.07475299614191	37.0	37.0	37.0	33.0	37.0
27	35.00167206005298	37.0	37.0	37.0	27.0	37.0
28	35.00328456123865	37.0	37.0	37.0	27.0	37.0
29	35.02997796321909	37.0	37.0	37.0	27.0	37.0
30	34.97586321754648	37.0	37.0	37.0	27.0	37.0
31	34.967322034795615	37.0	37.0	37.0	27.0	37.0
32	34.985414695157864	37.0	37.0	37.0	27.0	37.0
33	34.95237717086557	37.0	37.0	37.0	27.0	37.0
34	34.88868888874183	37.0	37.0	37.0	27.0	37.0
35	34.86487195946468	37.0	37.0	37.0	27.0	37.0
36	34.82621825457225	37.0	37.0	37.0	27.0	37.0
37	34.805507209931775	37.0	37.0	37.0	27.0	37.0
38	34.65225342077457	37.0	37.0	37.0	27.0	37.0
39	34.70936375688512	37.0	37.0	37.0	27.0	37.0
40	34.72738362307619	37.0	37.0	37.0	27.0	37.0
41	34.72349244374444	37.0	37.0	37.0	27.0	37.0
42	34.58813940304368	37.0	37.0	37.0	27.0	37.0
43	34.32529509212874	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	11.0
16	26.0
17	25.0
18	26.0
19	20.0
20	44.0
21	103.0
22	262.0
23	660.0
24	1395.0
25	2534.0
26	4065.0
27	6196.0
28	8751.0
29	11692.0
30	15020.0
31	19204.0
32	24176.0
33	30969.0
34	43897.0
35	77840.0
36	206417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58767837329292	20.06494122422149	13.716407144417017	23.630973258068572
2	16.718174057480926	23.584870282992856	37.9392190729552	21.75773658657102
3	17.846042533854806	28.499359190705288	30.1460515779791	23.508546697460808
4	11.86500872427112	18.354939966867622	40.845471209905305	28.934580098955955
5	13.651112978759544	37.249659742396865	34.60723132884657	14.491995949997023
6	27.27465240783265	41.88267785491019	16.834203554561437	14.008466182695722
7	26.040239735470394	32.772156450115034	23.10509051844891	18.082513295965658
8	24.557665115930234	34.377157630262964	20.093397127497887	20.971780126308918
9	27.18487293005363	14.004716179938367	20.03383826017519	38.77657262983282
10	16.229791345434812	26.941122750825553	32.873185936166124	23.955899967573504
11	33.8512748906433	24.618768102035368	23.313767142475843	18.21618986484549
12	23.981047044887532	26.972887480064323	28.329506124636854	20.716559350411288
13	29.31134508187138	22.67670785052048	25.107812579273954	22.904134488334183
14	22.930605096033158	21.345456871659465	28.373844392532643	27.350093639774737
15	25.280753882907263	27.39443190767052	25.939210249419304	21.38560396000291
16	22.108251550184963	28.10781478515793	25.61318059792691	24.170753066730196
17	22.312736994659556	26.271636964438944	25.83266605343092	25.582959987470577
18	23.91751758640999	25.28626859284455	28.173108950815408	22.623104869930053
19	26.337813483686386	24.470753287318594	28.619579867338135	20.571853361656885
20	25.58648940183044	24.50009154418496	28.487006240445766	21.426412813538835
21	22.970531595979114	26.782078516234204	28.316491409184856	21.930898478601822
22	22.71001669854169	26.218254572246007	28.584285723739505	22.487443005472798
23	22.641193118524352	26.141489809918976	29.37244806797652	21.84486900358015
24	22.648913712436553	26.76818144719224	27.419137808189564	23.163767032181642
25	22.83288443594444	26.566343063487547	29.36185982489693	21.238912675671084
26	22.81832560171	26.64707841696942	28.581418074572113	21.95317790674846
27	22.765163797914557	26.518254792834405	28.25671195346467	22.459869455786365
28	22.106707431402523	26.056342688487273	29.351492170214833	22.485457709895375
29	23.883767561593796	26.246048710329934	27.626270313434055	22.243913414642215
30	23.56281144324371	25.898180807485886	28.736271129611126	21.802736619659278
31	23.20523765091004	25.939651426214283	28.610094566246	22.245016356629673
32	21.192368523800383	25.989063227252373	29.19840382235575	23.62016442659149
33	21.445824592518083	26.11612214420746	29.789801316030378	22.64825194724408
34	22.560898941837458	25.415092216979573	29.301198015586778	22.722810825596195
35	22.51501655515923	26.726710828463844	28.535976864688873	22.222295751688055
36	23.45538489366536	26.396710585816606	27.80141750104228	22.34648701947575
37	22.862663869605786	25.925533768774827	28.147300108308904	23.064502253310483
38	22.79273734760099	26.370019389720138	27.809138094954484	23.028105167724387
39	22.090383889988153	26.151857464601076	28.66060930927155	23.097149336139218
40	22.037663262987696	26.787152049376505	29.30450684154915	21.87067784608665
41	22.683104914047732	25.35354805407945	28.543035693408598	23.42031133846422
42	21.657810042507382	27.197667057108134	28.49715330673037	22.647369593654112
43	22.677590204110444	25.52516582732781	28.648035770614538	23.149208197947203
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34.0
1	56.5
2	79.0
3	268.0
4	457.0
5	457.0
6	588.0
7	719.0
8	719.0
9	719.0
10	937.5
11	1156.0
12	1156.0
13	1735.0
14	2314.0
15	4072.0
16	5830.0
17	6223.0
18	6616.0
19	6616.0
20	5055.0
21	3494.0
22	2711.0
23	1928.0
24	2346.0
25	2764.0
26	2764.0
27	3708.0
28	4652.0
29	6314.5
30	7977.0
31	10709.5
32	13442.0
33	13442.0
34	16986.5
35	20531.0
36	22888.0
37	25245.0
38	27513.5
39	29782.0
40	29782.0
41	33152.5
42	36523.0
43	38397.0
44	40271.0
45	40189.5
46	40108.0
47	40108.0
48	39912.5
49	39717.0
50	39720.0
51	39723.0
52	37488.0
53	35253.0
54	35253.0
55	32972.0
56	30691.0
57	25847.0
58	21003.0
59	18858.0
60	16713.0
61	16713.0
62	13875.0
63	11037.0
64	8205.0
65	5373.0
66	4698.5
67	4024.0
68	4024.0
69	3128.5
70	2233.0
71	1734.0
72	1235.0
73	1069.5
74	904.0
75	904.0
76	585.0
77	266.0
78	226.5
79	187.0
80	157.0
81	127.0
82	127.0
83	107.0
84	87.0
85	73.0
86	59.0
87	48.5
88	38.0
89	38.0
90	26.5
91	15.0
92	10.0
93	5.0
94	3.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	453333.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.62655032806754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.60652749808966	29.42985609337587
2	6.9239914559179745	4.933558601507427
3	2.4684421507605876	2.638262355479861
4	1.4248266506144236	2.030466335075467
5	0.9242132254383328	1.64632644949722
6	0.6874934674146529	1.4695812370239478
7	0.5128368612450769	1.2789425773065148
8	0.4400864695192487	1.254301020402326
9	0.3556153158463237	1.140241225268762
>10	2.9699205023931716	21.393209144081784
>50	0.4048291836109383	9.98202711441199
>100	0.2663837834142205	17.733935537919677
>500	0.012979256268442298	2.9146999517673993
>1k	0.0018541794669203282	2.154592356881771
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4671	1.0303684046826505	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3394	0.748677021086045	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1720	0.3794120436853263	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	884	0.19500014338245836	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	862	0.19014719863764606	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	818	0.18044130914802145	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	811	0.17889719036558116	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	745	0.16433835613114423	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	687	0.15154422907663903	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	673	0.14845599151175848	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	614	0.1354412760597618	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	614	0.1354412760597618	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	588	0.12970597772498363	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	585	0.12904421253250922	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	579	0.1277206821475604	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	553	0.1219853838127822	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	543	0.11977949983786754	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	543	0.11977949983786754	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	542	0.11955891144037605	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	532	0.11735302746546138	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	524	0.11558832028552962	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	521	0.1149265550930552	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	516	0.11382361310559787	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	503	0.11095596393820878	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	497	0.10963243355325997	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	490	0.10808831477081969	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	483	0.1065441959883794	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	461	0.1016912512435671	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.001985295577423219	0.0	0.0
2	2.2058839749146875E-4	0.0	0.0022058839749146872	0.0	0.0
3	2.2058839749146875E-4	0.0	0.002647060769897625	0.0	0.0
4	2.2058839749146875E-4	0.0	0.002647060769897625	2.2058839749146875E-4	0.0
5	2.2058839749146875E-4	0.0	0.004191179552337906	4.411767949829375E-4	0.0
6	2.2058839749146875E-4	0.0	0.008823535899658749	4.411767949829375E-4	0.0
7	2.2058839749146875E-4	0.0	0.01742648340182603	4.411767949829375E-4	0.0
8	4.411767949829375E-4	0.0	0.018970602184266314	4.411767949829375E-4	0.0
9	8.82353589965875E-4	0.0	0.024264723724061563	8.82353589965875E-4	0.0
10	0.002867649167389094	0.0	0.026911784493959187	8.82353589965875E-4	0.0
11	0.002867649167389094	0.0	0.04654415187069991	0.0011029419874573436	0.0
12	0.003308825962372031	0.0	0.05095591982052928	0.0011029419874573436	0.0
13	0.003308825962372031	0.0	0.05514709937286719	0.0011029419874573436	0.0
14	0.003308825962372031	0.0	0.05977945572018803	0.00176470717993175	0.0
15	0.003308825962372031	0.0	0.06816181482486385	0.00176470717993175	0.0
16	0.003308825962372031	0.0	0.07566182033957378	0.00176470717993175	0.0
17	0.0035294143598635	0.0	0.07808829271197994	0.00176470717993175	0.0
18	0.0037500027573549688	0.0	0.08095594187936903	0.001985295577423219	0.0
19	0.0037500027573549688	0.0	0.08161770707184343	0.001985295577423219	0.0
20	0.0037500027573549688	0.0	0.08316182585428372	0.0022058839749146872	0.0
21	0.0037500027573549688	0.0	0.08647065181665575	0.0030882375648805622	0.0
22	0.0037500027573549688	0.0	0.08867653579157043	0.0037500027573549688	0.0
23	0.0037500027573549688	0.0	0.08933830098404484	0.004191179552337906	0.0
24	0.0037500027573549688	0.0	0.09022065457401071	0.004852944744812312	0.0
25	0.0037500027573549688	0.0	0.09066183136899365	0.005514709937286718	0.0
26	0.0037500027573549688	0.0	0.09066183136899365	0.006397063527252594	0.0
27	0.0037500027573549688	0.0	0.09066183136899365	0.007941182309692875	0.0
28	0.0037500027573549688	0.0	0.09088241976648512	0.024705900519044498	0.0
29	0.0037500027573549688	0.0	0.09088241976648512	0.06220592809259418	0.0
30	0.0037500027573549688	0.0	0.09088241976648512	0.10963243355325997	0.0
31	0.0037500027573549688	0.0	0.09088241976648512	0.2223531046714005	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTACT	25	0.0054928767	29.599998	4
GATTATC	25	0.0054928767	29.599998	2
TCGCAGC	40	5.932316E-5	27.75	37
AGCGTCA	35	8.861075E-4	26.428572	37
CCCACGC	35	8.861075E-4	26.428572	19
GCCGCTC	155	0.0	26.258064	27
GGTATCA	745	0.0	25.825502	1
CCGCTCT	160	0.0	25.4375	28
CGCTCTC	160	0.0	25.4375	29
CTTAGGA	45	1.3214858E-4	24.666666	2
CGCCGCC	125	0.0	23.68	33
GCTCTCT	180	0.0	23.63889	30
GCAGCTT	180	0.0	23.63889	19
CCGGCAG	165	0.0	23.545454	16
TTATACC	55	1.8993655E-5	23.545454	4
GTATCAA	2100	0.0	23.521427	1
CTTCGCC	190	0.0	23.368422	23
GCCGGCA	175	0.0	23.257141	15
TTAAGCA	40	0.0019293929	23.125	37
CCCTCTC	65	2.6768976E-6	22.76923	32
>>END_MODULE