FastQCFastQC Report
Fri 10 Feb 2017
ERR1632867.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632867.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414806
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19680.47443865324995305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14630.3526949947686388No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9390.22637088180980988No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG8260.1991292314961693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7870.18972724598969157No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7570.18249494944624717No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA7370.17767341841728423No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7190.17333404049121756No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6860.16537851429342873No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG6840.16489636119053244No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT6790.16369097843329172No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT6790.16369097843329172No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6690.16128021291881026No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG6500.15669975844129547No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6460.15573545223550286No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA6010.14488700742033625No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC5530.1333153329508252No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT5360.1292170315762067No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG5310.12801164881896596No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC5310.12801164881896596No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA5250.1265651895102771No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG5230.1260830364073808No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT5100.12294904123855488No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG5050.12174365848131416No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG4860.11716320400379937No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC4810.11595782124655862No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA4740.11427028538642162No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG4740.11427028538642162No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC4630.11161844332049199No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC4340.10462722332849574No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4270.1029396874683587No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG4250.10245753436546241No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA4240.10221645781401427No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT4180.10076999850532538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCTC658.731149E-1131.30769229
GCCGGCA751.4551915E-1129.615
CGATCAA250.00549234529.59999835
GCCGCTC702.055458E-1029.07142827
CCGCTCT754.6020432E-1027.13333328
GCTTCGC856.91216E-1126.11764722
AGCTTCG901.4188117E-1024.66666621
GGTATCA4500.024.2555561
CCGGCAG952.8012437E-1023.36842216
CGGCAGC952.8012437E-1023.36842217
GTATCAA12450.022.8835331
GTACTAT1005.311449E-1022.1999991
GTCCGGC502.69807E-422.19999915
CGCCGTC502.69807E-422.19999926
CGCCGGC1005.311449E-1022.19999914
TACACAG603.718899E-521.5833345
TTCGCCG1059.767973E-1021.14285724
GGCAGCT1151.3460522E-1020.91304218
CGGTTAT450.003821748320.55555524
GTCAACC1800.020.55555515
Produced by FastQC (version 0.11.5)