##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632867.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	414806
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.464231472061638	33.0	33.0	33.0	27.0	33.0
2	31.055941813763543	33.0	33.0	33.0	27.0	33.0
3	31.492736363504868	33.0	33.0	33.0	27.0	33.0
4	31.630603221747034	33.0	33.0	33.0	27.0	33.0
5	31.762255126492867	33.0	33.0	33.0	27.0	33.0
6	34.58337150378731	37.0	37.0	37.0	27.0	37.0
7	34.777066869813844	37.0	37.0	37.0	27.0	37.0
8	34.95231264735804	37.0	37.0	37.0	27.0	37.0
9	35.03160272512934	37.0	37.0	37.0	27.0	37.0
10	35.036535151371965	37.0	37.0	37.0	27.0	37.0
11	35.068950304479685	37.0	37.0	37.0	33.0	37.0
12	35.018977546129996	37.0	37.0	37.0	27.0	37.0
13	35.05733764699643	37.0	37.0	37.0	27.0	37.0
14	35.03249711913521	37.0	37.0	37.0	27.0	37.0
15	35.04218598573791	37.0	37.0	37.0	27.0	37.0
16	35.048878270806114	37.0	37.0	37.0	27.0	37.0
17	35.021369025520364	37.0	37.0	37.0	27.0	37.0
18	35.00896804771387	37.0	37.0	37.0	27.0	37.0
19	35.026964412279476	37.0	37.0	37.0	27.0	37.0
20	35.00952734531323	37.0	37.0	37.0	27.0	37.0
21	35.0212750056653	37.0	37.0	37.0	27.0	37.0
22	35.00660549750968	37.0	37.0	37.0	27.0	37.0
23	35.00184182485307	37.0	37.0	37.0	27.0	37.0
24	35.000313399516884	37.0	37.0	37.0	27.0	37.0
25	34.97465321138074	37.0	37.0	37.0	27.0	37.0
26	34.93751777939567	37.0	37.0	37.0	27.0	37.0
27	34.8795075288207	37.0	37.0	37.0	27.0	37.0
28	34.85028422925416	37.0	37.0	37.0	27.0	37.0
29	34.882089458686714	37.0	37.0	37.0	27.0	37.0
30	34.840007135865925	37.0	37.0	37.0	27.0	37.0
31	34.802353389295234	37.0	37.0	37.0	27.0	37.0
32	34.82833903077583	37.0	37.0	37.0	27.0	37.0
33	34.821444241404414	37.0	37.0	37.0	27.0	37.0
34	34.74794482239891	37.0	37.0	37.0	27.0	37.0
35	34.74472645043707	37.0	37.0	37.0	27.0	37.0
36	34.72055370462336	37.0	37.0	37.0	27.0	37.0
37	34.74530744492606	37.0	37.0	37.0	27.0	37.0
38	34.577306017752875	37.0	37.0	37.0	27.0	37.0
39	34.661010207181185	37.0	37.0	37.0	27.0	37.0
40	34.67981899972517	37.0	37.0	37.0	27.0	37.0
41	34.692902224172265	37.0	37.0	37.0	27.0	37.0
42	34.58900546279465	37.0	37.0	37.0	27.0	37.0
43	34.35259615338254	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	15.0
16	19.0
17	29.0
18	32.0
19	22.0
20	35.0
21	133.0
22	337.0
23	763.0
24	1535.0
25	2660.0
26	4195.0
27	6197.0
28	8574.0
29	11174.0
30	14735.0
31	18306.0
32	23004.0
33	29637.0
34	41615.0
35	73176.0
36	178611.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.66985530585382	21.641200946948693	13.555734487929296	24.133209259268188
2	16.01519746580329	23.5980193150533	38.34250227817341	22.044280940969998
3	16.959976470928577	28.568535652811196	30.7857166964798	23.685771179780428
4	11.676060616288096	19.035404502345674	41.32220845407251	27.966326427293726
5	12.828165455658791	37.8827692945618	36.56166979262595	12.727395457153465
6	27.05288737385669	42.75926577725491	17.327859288438454	12.859987560449945
7	25.47431811497423	33.09860513107332	24.432626336166784	16.994450417785664
8	23.34079063465813	36.34229013080814	21.021393133175508	19.295526101358227
9	25.87715703244408	15.119115924070528	21.139038490282207	37.86468855320319
10	15.169741999874638	27.907262672188928	34.6171463286452	22.305848999291236
11	33.02652324219032	24.627657266288335	24.55220030568507	17.79361918583627
12	22.79981485320849	27.14088031513527	29.658683818459714	20.400621013196528
13	27.030708331123463	23.409015298717957	26.332550638129632	23.227725732028947
14	21.612753913877814	21.717622213757757	28.636278163768125	28.033345708596308
15	23.890204095408457	29.0422510764068	25.893791314494003	21.173753513690738
16	21.957252305897214	29.224022796198707	26.08930439771845	22.72942050018563
17	21.512707145026834	27.858083055693506	26.777336875551462	23.8518729237282
18	22.15059570015863	27.896896380476655	28.619402805166754	21.333105114197963
19	24.571004276698023	26.451642454545016	28.292744077954513	20.684609190802448
20	23.72072727974041	26.472857191072453	28.754164597426268	21.05225093176087
21	21.88348288115408	28.256100442134397	28.42388972194231	21.436526954769217
22	21.860580608766508	27.941736619046008	28.200411758749873	21.997271013437608
23	21.787293337126272	27.73609832066074	29.29224746025853	21.184360881954454
24	22.30512576963689	28.53671354802004	26.520590348259187	22.637570334083883
25	22.369734285424993	28.033345708596308	28.568053499708295	21.0288665062704
26	22.02812881202297	28.291779771748722	28.41713957850176	21.26295183772655
27	22.191337637353364	27.890387313587556	27.562282127066627	22.35599292199245
28	21.323703128691484	27.569514423610077	29.42652709941515	21.680255348283296
29	23.570054435085318	27.476941027853986	27.778527793715618	21.174476743345082
30	22.94638939648896	26.76142582315589	28.7623612001755	21.52982358017965
31	22.321760051686812	27.539138778127608	28.30600328828416	21.83309788190142
32	20.786584572065014	27.178006104058284	28.865300887643862	23.17010843623284
33	20.948829091189616	27.35640275212991	29.546824298587776	22.1479438580927
34	22.243651249017613	26.870633500961894	29.20112052381113	21.68459472620936
35	21.87866135012512	28.203545753918704	28.23898400698158	21.678808888974604
36	22.991470711609765	27.857600902590608	27.477423180956883	21.673505204842748
37	21.869982594272987	27.055298139371175	28.165455658789888	22.909263607565947
38	22.057540151299644	27.39979653139058	28.09385592300979	22.448807394299987
39	21.488599489882017	27.495262845764046	28.798281606341273	22.21785605801266
40	21.503546236071802	28.28430639865383	28.932802322049344	21.279345043225025
41	21.861303838420852	26.566394893034335	28.64061754169419	22.931683726850626
42	21.111073610314218	28.187393624971673	28.560339050061955	22.141193714652154
43	22.430003423287033	26.360515518097614	28.446791994329878	22.762689064285475
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	28.5
2	28.0
3	100.0
4	172.0
5	172.0
6	241.0
7	310.0
8	324.0
9	338.0
10	419.5
11	501.0
12	501.0
13	787.5
14	1074.0
15	1890.0
16	2706.0
17	2998.0
18	3290.0
19	3290.0
20	2934.5
21	2579.0
22	2724.0
23	2869.0
24	3851.0
25	4833.0
26	4833.0
27	6353.5
28	7874.0
29	9692.0
30	11510.0
31	14735.0
32	17960.0
33	17960.0
34	21654.0
35	25348.0
36	28049.0
37	30750.0
38	32951.0
39	35152.0
40	35152.0
41	36700.5
42	38249.0
43	38994.5
44	39740.0
45	37539.0
46	35338.0
47	35338.0
48	33438.5
49	31539.0
50	30921.5
51	30304.0
52	27883.5
53	25463.0
54	25463.0
55	24206.0
56	22949.0
57	18418.0
58	13887.0
59	12686.5
60	11486.0
61	11486.0
62	9665.0
63	7844.0
64	5928.0
65	4012.0
66	3334.0
67	2656.0
68	2656.0
69	2107.0
70	1558.0
71	1283.5
72	1009.0
73	850.0
74	691.0
75	691.0
76	485.5
77	280.0
78	219.5
79	159.0
80	148.5
81	138.0
82	138.0
83	119.0
84	100.0
85	68.5
86	37.0
87	37.0
88	37.0
89	37.0
90	21.0
91	5.0
92	3.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	414806.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.579108051745536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.56674953789775	41.25360196517624
2	4.993349147209283	4.651714989359053
3	1.538492487637978	2.149848234554645
4	0.8485221798159569	1.5809362519180044
5	0.5861831260606255	1.3651943583443924
6	0.40476601922132965	1.1312184086991133
7	0.3317574496966914	1.0817076267475605
8	0.2534748897309864	0.9445307421747121
9	0.21355788433490083	0.8952602192763768
>10	1.8330616675132214	17.351739594791898
>50	0.235398799159535	7.737261611396275
>100	0.1821600463562698	15.552807535090096
>500	0.01148286825168463	3.4700372342328043
>1k	0.0010438971137895117	0.8341412282388251
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1968	0.47443865324995305	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1463	0.3526949947686388	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	939	0.22637088180980988	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	826	0.1991292314961693	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	787	0.18972724598969157	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	757	0.18249494944624717	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	737	0.17767341841728423	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	719	0.17333404049121756	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	686	0.16537851429342873	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	684	0.16489636119053244	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	679	0.16369097843329172	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	679	0.16369097843329172	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	669	0.16128021291881026	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	650	0.15669975844129547	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	646	0.15573545223550286	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	601	0.14488700742033625	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	553	0.1333153329508252	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	536	0.1292170315762067	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	531	0.12801164881896596	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	531	0.12801164881896596	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	525	0.1265651895102771	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	523	0.1260830364073808	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	510	0.12294904123855488	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	505	0.12174365848131416	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	486	0.11716320400379937	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	481	0.11595782124655862	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	474	0.11427028538642162	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	474	0.11427028538642162	No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC	463	0.11161844332049199	No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC	434	0.10462722332849574	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	427	0.1029396874683587	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG	425	0.10245753436546241	No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA	424	0.10221645781401427	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	418	0.10076999850532538	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0012053827572407342	0.0	0.0
2	0.0	0.0	0.0012053827572407342	0.0	0.0
3	0.0	0.0	0.001446459308688881	0.0	0.0
4	0.0	0.0	0.0019286124115851747	0.0	0.0
5	0.0	0.0	0.0024107655144814683	2.4107655144814684E-4	0.0
6	0.0	0.0	0.0053036841318592305	2.4107655144814684E-4	0.0
7	0.0	0.0	0.01446459308688881	4.821531028962937E-4	0.0
8	2.4107655144814684E-4	0.0	0.016152128947025838	4.821531028962937E-4	0.0
9	2.4107655144814684E-4	0.0	0.019286124115851747	7.232296543444405E-4	0.0
10	0.0012053827572407342	0.0	0.020973659975988775	9.643062057925874E-4	0.0
11	0.0012053827572407342	0.0	0.029170262725225767	9.643062057925874E-4	0.0
12	0.0012053827572407342	0.0	0.031339951688259086	9.643062057925874E-4	0.0
13	0.0012053827572407342	0.0	0.034232870305636855	9.643062057925874E-4	0.0
14	0.0012053827572407342	0.0	0.03640255926867017	9.643062057925874E-4	0.0
15	0.0012053827572407342	0.0	0.0380900951288072	9.643062057925874E-4	0.0
16	0.0012053827572407342	0.0	0.041706243400529405	0.001446459308688881	0.0
17	0.0012053827572407342	0.0	0.04315270270921828	0.001446459308688881	0.0
18	0.0012053827572407342	0.0	0.04411700891501087	0.001687535860137028	0.0
19	0.0012053827572407342	0.0	0.045322391672251605	0.001687535860137028	0.0
20	0.0012053827572407342	0.0	0.04604562132659605	0.001687535860137028	0.0
21	0.0012053827572407342	0.0	0.04749208063528493	0.001687535860137028	0.0
22	0.0012053827572407342	0.0	0.04773315718673308	0.002892918617377762	0.0
23	0.0012053827572407342	0.0	0.04797423373818122	0.003375071720274056	0.0
24	0.0012053827572407342	0.0	0.04797423373818122	0.0036161482717222025	0.0
25	0.001446459308688881	0.0	0.04797423373818122	0.0036161482717222025	0.0
26	0.001446459308688881	0.0	0.048215310289629366	0.00458045447751479	0.0
27	0.001446459308688881	0.0	0.048215310289629366	0.006026913786203671	0.0
28	0.001446459308688881	0.0	0.048215310289629366	0.0190450475644036	0.0
29	0.001446459308688881	0.0	0.04845638684107752	0.04845638684107752	0.0
30	0.001446459308688881	0.0	0.04845638684107752	0.08196602749236993	0.0
31	0.001446459308688881	0.0	0.04845638684107752	0.18538786806362492	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTCTC	65	8.731149E-11	31.307692	29
GCCGGCA	75	1.4551915E-11	29.6	15
CGATCAA	25	0.005492345	29.599998	35
GCCGCTC	70	2.055458E-10	29.071428	27
CCGCTCT	75	4.6020432E-10	27.133333	28
GCTTCGC	85	6.91216E-11	26.117647	22
AGCTTCG	90	1.4188117E-10	24.666666	21
GGTATCA	450	0.0	24.255556	1
CCGGCAG	95	2.8012437E-10	23.368422	16
CGGCAGC	95	2.8012437E-10	23.368422	17
GTATCAA	1245	0.0	22.883533	1
GTACTAT	100	5.311449E-10	22.199999	1
GTCCGGC	50	2.69807E-4	22.199999	15
CGCCGTC	50	2.69807E-4	22.199999	26
CGCCGGC	100	5.311449E-10	22.199999	14
TACACAG	60	3.718899E-5	21.583334	5
TTCGCCG	105	9.767973E-10	21.142857	24
GGCAGCT	115	1.3460522E-10	20.913042	18
CGGTTAT	45	0.0038217483	20.555555	24
GTCAACC	180	0.0	20.555555	15
>>END_MODULE